公开(公告)号：US20220356225A1

公开(公告)日：2022-11-10

申请号：US17763122

申请日：2020-09-23

Applicant: The Regents of the University of California ,  Trustees of Boston University

Inventor： Kole T. ROYBAL ,  Iowis ZHU ,  Raymond LIU ,  Ahmad S. KHALIL ,  Divya ISRANI

IPC: C07K14/705 ,  C07K16/28 ,  C07K14/725 ,  A61K35/17 ,  C12N5/0783

Abstract: The present disclosure generally relates to, inter alia, a new class of chimeric Notch receptors containing a synthetic zinc finger transcriptional effector (synZTE) module, engineered to modulate gene expression and cellular activities in a ligand-dependent manner. The new Notch receptors surprisingly retain the ability to transduce signals in response to ligand binding despite that the Notch extracellular subunit, which includes the negative regulatory region previously believed to be essential for the functioning of Notch receptors, is partly or completely deleted. In addition, the synZTE is designed to bind orthogonal DNA target sequences in target organisms which in turn facilitates precise regulation of therapeutic gene expression with minimal off-target activity. Also provided are compositions and methods useful for producing such receptors, nucleic acids encoding same, host cells genetically modified with the nucleic acids, as well as methods for modulating an activity of a cell and/or for treatment of various health conditions.

公开(公告)号：US11473104B2

公开(公告)日：2022-10-18

申请号：US15780668

申请日：2015-12-04

Applicant: Massachusetts Institute of Technology ,  Trustees of Boston University ,  President and Fellows of Harvard College

Inventor： Russell-John Krom ,  James J. Collins ,  Prerna Saluja Bhargava ,  Michael Andrew Lobritz

IPC: C12N15/86 ,  A61K35/76 ,  C07K14/245 ,  C07K14/35 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C07K14/46 ,  C07K14/435 ,  C07K14/61

Abstract: Provided herein, in some embodiments, are engineered phagemids that comprise at least one synthetic genetic circuit, wherein the at least one synthetic genetic circuit comprises gene sequences encoding at least one non-lytic antimicrobial peptides (AMPs) and/or antibacterial toxin proteins, a stable origin of replication, and a bacteriophage-packaging signal, wherein the engineered phagemid does not comprise some or all gene sequences encoding bacteriophage proteins required for assembly of a bacteriophage particle.

公开(公告)号：US11458132B2

公开(公告)日：2022-10-04

申请号：US17464260

申请日：2021-09-01

Applicant: TRUSTEES OF BOSTON UNIVERSITY

Inventor： Scott Edward Schaus ,  Ulla Hansen ,  Emily Ann York ,  Niranjana Pokharel

IPC: A61K31/4741 ,  A61P35/00 ,  C07D491/056 ,  C07D215/227

Abstract: The present invention is directed to compositions, methods and kits for treatment of cancer, e.g., hepatocellular carcinoma (HCC). In some embodiments, the present invention discloses the use of a small-molecule compounds of Formula (I) to inhibit tubulin acetylation, to inhibit cell migration, or to modulate cell compaction.

公开(公告)号：US20220308327A1

公开(公告)日：2022-09-29

申请号：US17704896

申请日：2022-03-25

Applicant: Trustees of Boston University

Inventor： Ji-Xin Cheng ,  Celalettin Yurdakul ,  Haonan Zong ,  M. Salim Unlü

IPC: G02B21/16 ,  G02B21/36 ,  G02B21/02

Abstract: Microscopic analysis of a sample includes a system using dark-field illumination. A mid-IR optical source generates a mid-infrared beam, which is directed onto the sample to induce a temperature change by absorption of the mid-infrared beam. A visible light source generates a light illuminating the sample on a substrate and creating a scattered field and a reflected field along a collection path of the system. A pupil mask is positioned along the collection path to block the reflected field while allowing the scattered field to pass therethrough. A camera is positioned at an end of the collection path to collect the scattered field and generate a dark-field image of the sample.

公开(公告)号：US11445960B2

公开(公告)日：2022-09-20

申请号：US17064691

申请日：2020-10-07

Applicant: Trustees of Boston University

Inventor： Mark Alan Kramer

IPC: A61B5/245 ,  A61B5/291 ,  A61B5/00 ,  A61B5/256 ,  A61B5/296

Abstract: An electrography system includes an array of conductive electrodes configured to be arranged into two or more spatially separated layers and generate respective electrode signals collectively conveying surface-parallel components and surface-orthogonal components of a pattern of physiological electrical activity sensed by the electrodes. The system further includes signal processing circuitry configured and operative to receive the electrode signals and to generate, based on the surface-parallel and surface-orthogonal components, a set of electrography signals representing the pattern of electrical activity; and a recording component configured and operative to record the electrography signals in a manner enabling application-specific use thereof.

公开(公告)号：US20220287758A1

公开(公告)日：2022-09-15

申请号：US17690948

申请日：2022-03-09

Applicant: Trustees of Boston University

Inventor： Chen Yang ,  Ji-Xin Cheng ,  Nan Zheng ,  Yueming Li ,  Ying Jiang ,  Lu Lan ,  Linli Shi

IPC: A61B18/04 ,  B82Y30/00 ,  B82Y40/00 ,  B01J19/10

Abstract: A tapered fiber optoacoustic emitter includes a nanosecond laser configured to emit laser pulses and an optic fiber. The optic fiber includes a tip configured to guide the laser pulses. The tip has a coating including a diffusion layer and a thermal expansion layer, wherein the diffusion layer includes epoxy and zinc oxide nanoparticles configured to diffuse the light while restricting localized heating. The thermal expansion layer includes carbon nanotubes (CNTs) and Polydimethylsiloxane (PDMS) configured to convert the laser pulses to generate ultrasound. The frequency of the ultrasound is tuned with a thickness of the diffusion layer and a CNT concentration of the expansion layer.

公开(公告)号：US11375918B2

公开(公告)日：2022-07-05

申请号：US17104787

申请日：2020-11-25

Applicant: BOSTON MEDICAL CENTER CORPORATION ,  TRUSTEES OF BOSTON UNIVERSITY

Inventor： Hernan Jara ,  Ryan McNaughton

IPC: G01V3/00 ,  A61B5/055 ,  G01R33/56 ,  A61B5/00 ,  G01R33/563 ,  G01R33/48 ,  G01R33/483 ,  G06T7/00 ,  G06T11/00 ,  G01R33/50

Abstract: Methods of characterizing the brain of a subject, comprising: (a) performing a multispectral multislice magnetic resonance scan on the brain of a subject, (b) storing image data indicative of a plurality of magnetic resonance weightings of each of a plurality of slices of the brain of the subject to provide directly acquired images, (c) processing the directly acquired images to generate a plurality of quantitative maps of the brain indicative of a plurality of qMRI parameters of the subject, (d) constructing a plurality of magnetic resonance images indicative of white matter structure from the quantitative maps, and (e) generating a spatial entropy map of the brain of the subject from the plurality of magnetic resonance images; and/or generating a myelin water map of the brain of the subject from the plurality of magnetic resonance images.

公开(公告)号：US20220204579A1

公开(公告)日：2022-06-30

申请号：US17549845

申请日：2021-12-13

Applicant: THE JOHNS HOPKINS UNIVERSITY ,  TRUSTEES OF BOSTON UNIVERSITY

Inventor： William R. Bishai ,  John R. Murphy ,  Laurene Cheung ,  Shashank Gupta ,  Cynthia K. Bullen

IPC: C07K14/55 ,  C07K14/34 ,  C12N15/77

Abstract: The present invention is a DNA expression vector comprising: a toxP: a mutant toxO that blocks Fe-mediated regulation of gene expression; and a DNA sequence encoding a protein, wherein the toxP and the mutant toxO regulate expression of the DNA segment encoding the protein. It is preferred that DNA expression vectors of the present invention include DNA sequences encoding a signal peptide so that a protein expressed is attached to the signal peptide prior to processing. Novel proteins are produced off of the DNA expression vector of the present invention.

公开(公告)号：US11360104B2

公开(公告)日：2022-06-14

申请号：US16209678

申请日：2018-12-04

Applicant: TRUSTEES OF BOSTON UNIVERSITY ,  L'UNIVERSITÉ DE BORDEAUX ,  L'INSTITUT POLYTECHNIQUE DE BORDEAUX ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

Inventor： James Galagan ,  Allison Dennis ,  Catherine Klapperich ,  Mark Grinstaff ,  Thuy Nguyen ,  R. Baer ,  Uros Kuzmanovic ,  Marjon Zamani ,  Mingfu Chen ,  Margaret Chern ,  Chloe Grazon

IPC: G01N33/74 ,  C07K14/245 ,  G01N33/566 ,  G01N33/542 ,  C07K14/195 ,  C12N9/00 ,  C07K14/34 ,  C12N9/22

Abstract: Described herein are methods, compositions and processes related to a microbial-based biosensor system for the detection of small molecules and analytes based on an analyte-responsive transcription factor-DNA binding mechanism with either a ratiometric fluorescent output through Förster resonance energy transfer (FRET) or a redox sensor output for the quantification of the target analyte with high sensitivity.

公开(公告)号：US20220144939A1

公开(公告)日：2022-05-12

申请号：US17501050

申请日：2021-10-14

Applicant: TRUSTEES OF BOSTON UNIVERSITY

Inventor： Nelson RUIZ-OPAZO ,  Victoria HERRERA

IPC: C07K16/24 ,  A61K45/06 ,  A61P31/14

Abstract: Described herein are methods of treating COVID19 from a coronavirus infection, methods of treating a subject having a coronavirus infection, methods of improving a survival rate of a subject having a coronavirus infection, methods of determining prognosis of a subject having a coronavirus infection, methods of preventing or treating organ-dysfunction or multi-organ dysfunction or failure associated with a coronavirus infection, methods of combinational therapy for a subject having a coronavirus infection, methods of reducing microthrombi formation or low flow organ-ischemia associated with a coronavirus infection by administering a DEspR inhibitor.