公开(公告)号：US20060110725A1

公开(公告)日：2006-05-25

申请号：US11190169

申请日：2005-07-26

Applicant: Jeong-gun Lee ,  Young-nam Kwon ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Young-a Kim

Inventor： Jeong-gun Lee ,  Young-nam Kwon ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Young-a Kim

IPC: C12Q1/70 ,  C12Q1/68 ,  C12N7/02 ,  C12N1/08 ,  C12M1/34

CPC classification number: B01L3/502753 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487

Abstract: An apparatus for and method of purifying nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples, magnetic beads, and a solid support are introduced; a vibrator attached to the capillary and mixing the samples, magnetic beads, and solid support in the capillary; a laser generator attached to the capillary and irradiating a laser beam onto the capillary; a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall; a waste chamber attached to the capillary and discharging a lysate; an elution buffer chamber attached to the capillary and eluting nucleic acids from the solid support having nucleic acids bound thereto; and a neutralization buffer chamber attached to the capillary and supplying a neutralization buffer for neutralizing the eluted nucleic acid solution. According to the apparatus and method, PCR inhibitors can be removed to increase PCR yield and nucleic acids can be purified using a silicon substrate or silica beads. Thus, the apparatus and method can be applied to LOC fabrication.

Abstract translation: 提供了一种纯化细胞或病毒核酸的装置和方法。 核酸纯化装置包括：细胞裂解毛细管，其具有样品入口，通过所述样品入口引入样品，磁珠和固体支持物; 连接到毛细管并将样品，磁珠和固体支持物混合在毛细管中的振动器; 激光发生器附接到毛细管并将激光束照射到毛细管上; 附着到毛细管并将磁珠固定到毛细管壁的磁力发生器; 连接到毛细管并排出裂解物的废物室; 洗脱缓冲室连接到毛细管并从具有与其结合的核酸的固体支持物洗脱核酸; 以及中和缓冲室，其连接到毛细管并提供用于中和洗脱的核酸溶液的中和缓冲液。 根据该装置和方法，可以除去PCR抑制剂以增加PCR产量，并且可以使用硅底物或二氧化硅珠纯化核酸。 因此，该装置和方法可以应用于LOC制造。

公开(公告)号：US20060089961A1

公开(公告)日：2006-04-27

申请号：US11134615

申请日：2005-05-19

Applicant: Jeong-A Lee ,  Ki-Seon Kim ,  Jeong-Gun Lee ,  Suk-Jin Kim

Inventor： Jeong-A Lee ,  Ki-Seon Kim ,  Jeong-Gun Lee ,  Suk-Jin Kim

IPC: G06F7/50

CPC classification number: G06F7/506 ,  G06F7/507 ,  G06F7/508

Abstract: Disclosed is a mixed-type adder with optimized design costs. The mixed-type adder includes I sub adders, (where, I is a positive number larger than 1). An overall bit width of the mixed-type adder is divided into I bit groups which are respectively allocated to the I sub adders. The I sub adders have different carry propagation schemes and are connected in series through a carry signal.

公开(公告)号：US20060007026A1

公开(公告)日：2006-01-12

申请号：US10967647

申请日：2004-10-14

Applicant: Eun-Gu Jung ,  Jeong-Gun Lee ,  Dong-Soo Har

Inventor： Eun-Gu Jung ,  Jeong-Gun Lee ,  Dong-Soo Har

IPC: H03M7/00

CPC classification number: H03M7/3044 ,  H03M7/18

Abstract: Disclosed is a data transmitting circuit and a method based on a differential value data encoding to reduce a data transmitting time by transmitting an encoded differential value. The circuit comprises an encoder for encoding and outputting a differential value between a currently transmitted data value and a previously transmitted data value, wherein the encoder inverts a phase of one output signal among 2n(namely, N)-output signals in response to n-bit input value and outputs an encoded data value; and a decoder for decoding the output value of the encoder and restoring the original data value, wherein the decoder restores the original data value by adding an output value from the encoder and the previous original data value.

Abstract translation: 公开了一种数据发送电路和基于差分值数据编码的方法，以通过发送编码的差分值来减少数据发送时间。 该电路包括一个编码器，用于对当前传输的数据值和先前发送的数据值之间的差分值进行编码和输出，其中编码器将两个输出信号的相位反相（即N） - 响应于n位输入值输出信号并输出​​编码数据值; 以及解码器，用于对编码器的输出值进行解码并恢复原始数据值，其中解码器通过将来自编码器的输出值和先前的原始数据值相加来恢复原始数据值。

公开(公告)号：US20050186602A1

公开(公告)日：2005-08-25

申请号：US11042376

申请日：2005-01-25

Applicant: Joon-ho Kim ,  Yoon-kyoung Cho ,  Jung-joo Hwang ,  Geun-bae Lim ,  Jeong-gun Lee

Inventor： Joon-ho Kim ,  Yoon-kyoung Cho ,  Jung-joo Hwang ,  Geun-bae Lim ,  Jeong-gun Lee

IPC: C12N15/10 ,  C12P19/34 ,  C12Q1/68

CPC classification number: C12N15/1006

Abstract: A method for amplifying a nucleic acid using a solid phase material coated with a carboxyl group or an amino group is provided. The method includes contacting a mixture of a nucleic acid containing sample and a salt solution with the solid phase material coated with a carboxyl group or an amino group to form a nucleic acid-solid phase material complex, washing the nucleic acid-solid phase material complex with a wash buffer, and adding a reaction solution for amplifying a nucleic acid to the nucleic acid-solid phase material complex to perform an amplification reaction.

Abstract translation: 提供了使用涂覆有羧基或氨基的固相材料来扩增核酸的方法。 该方法包括将含有核酸的样品和盐溶液的混合物与涂覆有羧基或氨基的固相材料接触以形成核 - 固相物质络合物，洗涤核酸 - 固相物质络合物 用洗涤缓冲液加入用于将核酸扩增至核酸 - 固相物质络合物的反应溶液进行扩增反应。

公开(公告)号：US09726685B2

公开(公告)日：2017-08-08

申请号：US13269249

申请日：2011-10-07

Applicant: Beom-seok Lee ,  Yoon-kyoung Cho ,  Jong-myeon Park ,  Jeong-gun Lee

Inventor： Beom-seok Lee ,  Yoon-kyoung Cho ,  Jong-myeon Park ,  Jeong-gun Lee

IPC: B01L3/00 ,  G01N35/00 ,  F16K99/00

CPC classification number: B01L3/502738 ,  B01L3/502723 ,  B01L3/502746 ,  B01L3/502753 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0803 ,  B01L2300/0806 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0409 ,  B01L2400/0677 ,  B01L2400/084 ,  B01L2400/086 ,  F16K99/0001 ,  F16K99/0019 ,  F16K99/0032 ,  F16K99/004 ,  F16K99/0061 ,  F16K2099/0084 ,  G01N35/00069

Abstract: Provided is a rotatable microfluidic device for conducting simultaneously two or more assays. The device includes a platform which can be rotated, a first unit which is disposed at one portion of the platform and detects a target material from a sample using surface on which a capture probe selectively binds to the target material is attached, and a second unit which is disposed at another portion of the platform and detects a target material included in the sample by a different reaction from the reaction conducted in the first unit.

公开(公告)号：US09180418B2

公开(公告)日：2015-11-10

申请号：US11751677

申请日：2007-05-22

Applicant: Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park

Inventor： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park

IPC: B01F11/00 ,  B01F13/00 ,  B01F15/02

CPC classification number: B01F13/0059 ,  B01F11/0002 ,  B01F11/0014 ,  B01F15/0201 ,  B01F15/0233

Abstract: Provided is a method of mixing fluids including introducing at least two kinds of fluids to a chamber of a substrate including a microchannel structure; and alternately rotating the substrate clockwise and counter-clockwise until the at least two kinds of fluids are mixed, wherein the rotation is changed from one direction to the opposite direction before a vortex created in the mixing chamber by the one direction rotation disappears.

Abstract translation: 提供了一种混合流体的方法，包括将至少两种流体引入到包括微通道结构的基板的室中; 并且顺时针和逆时针地交替地旋转衬底，直到混合至少两种流体，其中在通过单向旋转在混合室中产生的涡流消失之前，旋转从一个方向改变到相反的方向。

公开(公告)号：US08658359B2

公开(公告)日：2014-02-25

申请号：US13079926

申请日：2011-04-05

Applicant: In-ho Lee ,  Jun-hong Min ,  Young-rok Kim ,  Jae-ho You ,  Chang-eun Yoo ,  Jeong-gun Lee ,  Ki-woong Han

Inventor： In-ho Lee ,  Jun-hong Min ,  Young-rok Kim ,  Jae-ho You ,  Chang-eun Yoo ,  Jeong-gun Lee ,  Ki-woong Han

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/00

CPC classification number: C12Q1/68 ,  C12M47/06 ,  C12N15/1006

Abstract: Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.

Abstract translation: 本文提供了在单个芯片中破坏细胞并纯化核酸的方法和装置。 该方法包括用激光束照射芯片，其中芯片包括其上沉积有细胞裂解增强金属氧化物层和细胞结合金属氧化物层的固体支持物。

公开(公告)号：US08590710B2

公开(公告)日：2013-11-26

申请号：US13156630

申请日：2011-06-09

Applicant: Tae-seok Sim ,  Jeong-gun Lee ,  Hyo-il Jung ,  Ki-ho Kwon

Inventor： Tae-seok Sim ,  Jeong-gun Lee ,  Hyo-il Jung ,  Ki-ho Kwon

IPC: B07C5/00

CPC classification number: B01L3/502761 ,  B01L3/502753 ,  B01L2200/0652 ,  B01L2300/087 ,  B01L2400/0487

Abstract: A device separating target particles in a fluid sample includes first through third multi-orifice flow fractionation (“MOFF”) channels, each including a multi-orifice segment with an inlet and an outlet at opposite ends, and an alternating series of contraction channels and expansion chambers interconnected in a lengthwise direction; a first separation unit including a first separation channel which is interconnected in fluid communication with a center region of the outlet of the first MOFF channel, and first branch channels which are interconnected in fluid communication with sidewall regions of the outlet of the first MOFF channel, and respectively with inlets of the second and third MOFF channels; and buffer inlets which are connected to the inlets of the second and third MOFF channels and through which a buffer flows into the second and third MOFF channels.

Abstract translation: 分离流体样品中的目标颗粒的装置包括第一至第三多孔流动分馏（“MOFF”）通道，每个通道包括具有相对端的入口和出口的多孔段，以及交替的一系列收缩通道和 膨胀室沿长度方向互相连接; 第一分离单元，其包括与第一MOFF通道的出口的中心区域流体连通的第一分离通道和与第一MOFF通道的出口的侧壁区域流体连通地互连的第一分支通道， 并分别具有第二和第三MOFF通道的入口; 以及连接到第二和第三MOFF通道的入口并且缓冲器通过其流入第二和第三MOFF通道的缓冲器入口。

公开(公告)号：US20130122577A1

公开(公告)日：2013-05-16

申请号：US13577418

申请日：2011-02-07

Applicant: Joo-won Rhee ,  Su-hyeon Kim ,  Jeong-gun Lee ,  Mi-jeong Song

Inventor： Joo-won Rhee ,  Su-hyeon Kim ,  Jeong-gun Lee ,  Mi-jeong Song

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  B82Y15/00 ,  G01N33/5308 ,  Y10S977/774 ,  Y10S977/92 ,  C12Q2522/101 ,  C12Q2563/107

Abstract: Provided are kits for determining a nucleotide sequence of a target nucleic acid, the kit including at least one sequence specific binding protein and a detectable tag. In accordance with a kit for determining a nucleotide sequence of a target nucleic acid according to one exemplary embodiment and a method and device for determining a nucleotide sequence of a target nucleic acid, the nucleotide sequence of the target nucleic acid may be more efficiently determined.

Abstract translation: 提供了用于确定靶核酸的核苷酸序列的试剂盒，该试剂盒包括至少一种序列特异性结合蛋白和可检测标签。 根据一个示例性实施方案的用于确定靶核酸的核苷酸序列的试剂盒以及用于确定靶核酸的核苷酸序列的方法和装置，可以更有效地确定靶核酸的核苷酸序列。

公开(公告)号：US20120325749A1

公开(公告)日：2012-12-27

申请号：US13434252

申请日：2012-03-29

Applicant: Minseok S. KIM ,  Won-ho LEE ,  Sun-soo KIM ,  Jin-hoon KIM ,  Jeong-gun LEE ,  Tae-seok SIM

Inventor： Minseok S. KIM ,  Won-ho LEE ,  Sun-soo KIM ,  Jin-hoon KIM ,  Jeong-gun LEE ,  Tae-seok SIM

IPC: B01D33/00 ,  B01D15/00 ,  B01D33/41 ,  B01D37/00 ,  B82Y99/00

CPC classification number: B01L3/502753 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2400/086

Abstract: A hydrodynamic filter unit includes an inlet channel connected to a fluid chamber, into which a fluid including a target material is introduced, and a plurality of outlet channels connected to the fluid chamber, through which the fluid is discharged. A filtering method includes introducing a fluid including a target material into the hydrodynamic filter unit through the inlet channel, capturing the target material in the hydrodynamic filter unit, and discharging a remaining part of the fluid outside of the hydrodynamic filter unit through an outlet channel.

Abstract translation: 流体动力过滤器单元包括连接到流体室的入口通道，包括目标材料的流体被引入到该流体室中，以及连接到流体室的多个出口通道，流体被排出。 过滤方法包括通过入口通道将包括目标材料的流体引入到流体动力过滤器单元中，捕获流体动力过滤器单元中的目标材料，以及通过出口通道将流体动力过滤器单元外剩余部分排出。