公开(公告)号：US12036529B2

公开(公告)日：2024-07-16

申请号：US17477340

申请日：2021-09-16

Applicant: Waters Technologies Corporation

Inventor： Yeliz Tunc Sarisozen ,  Nicole L Lawrence ,  Darryl W. Brousmiche ,  Andrew Wyatt Schmudlach ,  Matthew A. Lauber

IPC: B01J20/28 ,  B01J20/10 ,  B01J20/26 ,  B01J20/289

CPC classification number: B01J20/289 ,  B01J20/103 ,  B01J20/262 ,  B01J20/265 ,  B01J20/28004 ,  B01J20/28011 ,  B01J20/28016 ,  B01J20/28052 ,  B01J20/28083 ,  B01J20/28085 ,  B01J2220/4806 ,  B01J2220/54 ,  B01J2220/58

Abstract: Stationary phase materials for performing size exclusion chromatography are provided. The stationary phase materials include porous inorganic-organic hybrid particles surface-modified with a hydroxy-terminated polyethylene glycol silane.

公开(公告)号：US11874256B2

公开(公告)日：2024-01-16

申请号：US17471449

申请日：2021-09-10

Applicant: Waters Technologies Corporation

Inventor： Amit Patel ,  Moon Chul Jung ,  Thomas H. Walter ,  Matthew A. Lauber ,  Kevin Wyndham ,  Mathew DeLano ,  Jennifer Simeone

IPC: G01N30/16 ,  G01N30/72 ,  G01N30/74 ,  G01N30/86 ,  G01N30/06 ,  G01N30/02

CPC classification number: G01N30/16 ,  G01N30/72 ,  G01N30/74 ,  G01N30/8637 ,  G01N30/06 ,  G01N2030/027

Abstract: The present disclosure is directed to methods for evaluating system inertness, such as the inertness of a LC or other fluidic system. Some methods are directed to tests wherein the column has been removed prior to injecting a sample including a positive (e.g., metal reacting moiety) control into the system. Some methods can include: (1) repeatedly injecting the sample into a system, the system comprising: fluidic paths wherein interior surfaces of the fluidic paths define wetted surfaces, and wherein at least a portion of the wetted surfaces of the fluidic flow path are coated with an inert coating, wherein the inert coating is inert to at least one analyte in the sample; (2) detecting a value associated with the positive control; and (3) analyzing values associated with the detected positive control to determine system inertness.

公开(公告)号：US20230366860A1

公开(公告)日：2023-11-16

申请号：US18196575

申请日：2023-05-12

Applicant: Waters Technologies Corporation

Inventor： Szabolcs Fekete ,  Matthew A. Lauber

IPC: G01N30/52 ,  G01N30/56 ,  B01D15/38 ,  B01D15/16

CPC classification number: G01N30/52 ,  G01N30/56 ,  B01D15/3847 ,  B01D15/166 ,  G01N2030/562 ,  G01N2030/027

Abstract: The present disclosure is directed to liquid chromatography columns and methods utilizing a stationary phase sorbent having two or more gradient factors. Each of the two or more gradient factors varies in a progressive manner along a length of the stationary phase sorbent in a direction from an inlet to an outlet of the column (i.e., along a length of the column). As a result of including these new continuous stationary phase gradients, new selectivities allowing for separation and analysis of complex samples including large biomolecules is achievable.

公开(公告)号：US11709156B2

公开(公告)日：2023-07-25

申请号：US16746031

申请日：2020-01-17

Applicant: Waters Technologies Corporation

Inventor： Xiaoxiao Liu ,  Anna Boardman ,  Mathew H. DeLano ,  Matthew A. Lauber

IPC: G01N30/56 ,  G01N30/52 ,  B05D1/00 ,  G01N30/60 ,  G01N33/74 ,  G01N33/68 ,  G01N33/92

CPC classification number: G01N30/56 ,  B05D1/60 ,  G01N30/52 ,  G01N30/6052 ,  B05D2202/00 ,  B05D2203/35 ,  B05D2518/10 ,  G01N33/6803 ,  G01N33/74 ,  G01N33/92 ,  G01N2030/567

Abstract: A device for processing samples is disclosed. Interior surfaces of the device, which come in contact with fluids, define wetted surfaces. A portion of the wetted surfaces are coated with an alkylsilyl coating having the Formula I:




R1, R2, R3, R4, R5, and R6 are each independently selected from (C1-C6)alkoxy, —NH(C1-C6)alkyl, —N((C1-C6)alkyl)2, OH, ORA, and halo. RA represents a point of attachment to the interior surfaces of the fluidic system. At least one of R1, R2, R3, R4, R5, and R6 is ORA. X is (C1-C20)alkyl, —O[(CH2)2O]1-20—, —(C1-C10)[NH(CO)NH(C1-C10)]1-20—, or —(C1-C10)[alkylphenyl(C1-C10)alkyl]1-20—.

公开(公告)号：US20230125093A1

公开(公告)日：2023-04-27

申请号：US17970874

申请日：2022-10-21

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Kevin Wyndham ,  Szabolcs Fekete ,  Hua Yang ,  Stephan Koza

IPC: C12N15/10

Abstract: The present invention relates to a method for separating a sample comprising a nucleic acid, the method comprising: (a) loading a sample comprising a nucleic acid onto an anion-exchange column; and (b) eluting the sample from the anion-exchange column using a salt gradient, wherein a mobile phase used in the eluting step comprises a mild ion-pairing cation, and wherein a column temperature of the anion-exchange column is greater than 30° C. The present invention also relates to a method for determining one or more calorimetric properties of a nucleic acid using anion-exchange chromatography.

公开(公告)号：US11612885B2

公开(公告)日：2023-03-28

申请号：US16406816

申请日：2019-05-08

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： Qi Wang ,  Matthew A. Lauber

IPC: B01J49/53 ,  B01J39/05 ,  B01D15/36 ,  B01J39/18 ,  B01J39/26 ,  B01J47/02 ,  C07K1/18 ,  C07K16/24 ,  C07K16/28 ,  C07K16/32 ,  G01N30/08 ,  B01J47/022

Abstract: The present disclosure relates to methods, compositions and kits useful for the enhanced pH gradient cation exchange chromatography of a variety of analytes. In various aspects, the present disclosure pertains to chromatographic elution buffer solutions that comprise a first buffer salt, a second buffer salt, a third buffer salt, and fourth buffer salt. The first buffer salt may be, for example, a diprotic acid buffer salt, the second buffer salt may be, for example, a divalent buffer salt with two amine groups, the third buffer salt may be, for example, a monovalent buffer salt comprising a single amine group, and the fourth buffer salt may be, for example, a zwitterionic buffer salt. Moreover, the buffer solution has a pH ranging from 3 to 11.

公开(公告)号：US11597789B2

公开(公告)日：2023-03-07

申请号：US16165134

申请日：2018-10-19

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Stephan M. Koza ,  Pamela C. Iraneta ,  KEvin D. Wyndham

IPC: B01D15/30 ,  B01J20/286 ,  C08F230/08 ,  C08L33/14 ,  B01J20/32 ,  G01N30/06 ,  G01N30/02

Abstract: The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances.

公开(公告)号：US11478727B2

公开(公告)日：2022-10-25

申请号：US16848234

申请日：2020-04-14

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Jennifer M. Nguyen ,  Ximo Zhang ,  Nilini Ranbaduge ,  Robert Birdsall ,  Henry Shion ,  Melvin Blaze Muttikal Thomas ,  Thomas Walter

IPC: B01D15/16 ,  B01D15/30 ,  B01D15/38 ,  B01D3/02 ,  G01N30/72 ,  B01J20/26 ,  B01J20/283 ,  B01J20/22 ,  B01J20/285 ,  B01J20/28 ,  C07C67/54

Abstract: The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. A mobile phase is flowed through a chromatography column. The mobile phase includes about 0.005% (v/v) to about 2.50% (v/v) difluoroacetic acid and less than about 100 ppb of any individual impurity, especially metal impurities. A sample including the analyte is injected into the mobile phase. The analyte is separated from the sample.

公开(公告)号：US11352325B2

公开(公告)日：2022-06-07

申请号：US15005619

申请日：2016-01-25

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Matthew A. Lauber

IPC: G01N33/00 ,  C07D207/46 ,  G01N33/533 ,  C07D401/12 ,  C07D405/12 ,  C07D491/22

Abstract: Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described. In particular embodiments, the MS active, fluorescent molecules are of the compound of the Formula III or of the Formula IV where R1, R2 and R3 are defined herein.

公开(公告)号：US20220091076A1

公开(公告)日：2022-03-24

申请号：US17484157

申请日：2021-09-24

Applicant: Waters Technologies Corporation

Inventor： Mathew DeLano ,  Matthew A. Lauber ,  Kevin Wyndham ,  Jennifer M. Nguyen

IPC: G01N30/60 ,  C23C16/02 ,  C23C16/04 ,  C23C16/26

Abstract: The present disclosure relates to a method of reducing degradation of sample components in a liquid chromatography system. The method utilizes a masked metal frit to prevent or reduce metal surfaces from becoming catalytically active. The masked metal frit is a metal based frit that includes a coating on its exterior surfaces to mask or prevent contact between the organic solvents (and/or any analyte or other related solvent) and the underlying metal. The coating is a vapor deposited inorganic-organic hybrid coating, such as a vapor deposited C2 coating.