公开(公告)号：US09493433B2

公开(公告)日：2016-11-15

申请号：US13811925

申请日：2011-08-09

Applicant: Haruyo Soya ,  Tomomi Murakami ,  Haruki Tsunoda ,  Yu Ohsugi ,  Ayako Yoda ,  Masashi Matsushita

Inventor： Haruyo Soya ,  Tomomi Murakami ,  Haruki Tsunoda ,  Yu Ohsugi ,  Ayako Yoda ,  Masashi Matsushita

IPC: C09B21/00 ,  C09B67/00 ,  C09B67/42 ,  C07D279/30 ,  C09B67/28 ,  C09B67/44 ,  C12Q1/28 ,  G01N33/72 ,  C09B11/12

CPC classification number: C07D279/30 ,  C09B11/12 ,  C09B21/00 ,  C09B67/0077 ,  C09B67/0083 ,  C12Q1/28 ,  G01N33/723

Abstract: Described is a method for preserving an aqueous solution comprising a leuco chromogen, comprising adding at least one compound selected from the group consisting of polyoxyethylene alkylamine and polyoxyethylene alkenylamine to the aqueous solution containing a leuco chromogen, and a method for stabilizing a leuco chromogen, comprising allowing the leuco chromogen to coexist in an aqueous solution comprising at least one compound selected from the group consisting of polyoxyethylene alkylamine and polyoxyethylene alkenylamine.

Abstract translation: 描述了一种保存含有无色致发色素的水溶液的方法，包括将至少一种选自聚氧乙烯烷基胺和聚氧乙烯链烯基胺的化合物加入到含有无色致发色素的水溶液中，以及一种稳定无色发色体的方法，包括 允许无色发色体共存于包含至少一种选自聚氧乙烯烷基胺和聚氧乙烯链烯基胺的化合物的水溶液中。

公开(公告)号：US09365888B2

公开(公告)日：2016-06-14

申请号：US13707725

申请日：2012-12-07

Applicant: Stanley L. Hazen ,  Renliang Zhang

Inventor： Stanley L. Hazen ,  Renliang Zhang

IPC: C12Q1/28 ,  G01N33/68

CPC classification number: G01N33/573 ,  C12Q1/28 ,  G01N33/6893 ,  G01N2333/908 ,  G01N2800/32 ,  G01N2800/50 ,  G01N2800/56

Abstract: Methods for characterizing the near term risk of experiencing a major adverse cardiac event in a patient presenting to an Emergency Department with chest pain are provided. In one embodiment the method comprises determining the level of myeloperoxidase (MPO) activity and/or mass in a bodily sample obtained from the patient. Levels of MPO activity or MPO mass in bodily samples from the test subject are then compared to a control value in comparable bodily samples obtained from a control population. Such comparison can also be used to determine the near term treatment of the patient.

Abstract translation: 提供了表现出急性部位患有胸痛的患者出现主要不良心脏事件的近期风险的方法。 在一个实施方案中，该方法包括确定从患者获得的身体样品中髓过氧化物酶（MPO）活性和/或质量的水平。 然后将来自测试对象的身体样本中的MPO活性或MPO质量的水平与从对照群体获得的可比较的身体样品中的对照值进行比较。 这种比较也可用于确定患者的近期治疗。

公开(公告)号：US09315854B2

公开(公告)日：2016-04-19

申请号：US14151716

申请日：2014-01-09

Applicant: Advanced Cell Diagnostics, Inc.

Inventor： Xingyong Wu ,  Huei-Yu Fay Wang ,  Nan Su ,  Li-Chong Wang ,  Yuling Luo

IPC: C12Q1/68 ,  C12Q1/00 ,  G01N33/53 ,  C12Q1/28 ,  C12P19/34 ,  C12M1/34 ,  G01N31/22 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6841 ,  C12Q1/6818 ,  C12Q1/682 ,  C12Q2563/131 ,  C12Q2537/143 ,  C12Q2537/125

Abstract: Disclosed is a method for in situ detection of one or more target nucleic acids based on a combination of an in situ hybridization (ISH) assay method and a general ISH signal amplification method. This new method produces high signal intensity and while keeps low background noise of signal amplification. The result can be consistently reproduced and the method can be easily adopted for routine clinic diagnostic use. Further, the invention relates to a kit, comprising the components of the ISH assay and a general ISH signal amplification assay, for sensitive detection of one or more target nucleic acids.

Abstract translation: 公开了一种基于原位杂交（ISH）测定方法和一般ISH信号扩增方法的组合原位检测一种或多种靶核酸的方法。 这种新方法产生高信号强度，同时保持低信号放大的背景噪声。 结果可以一贯重现，该方法可以方便地用于常规诊所诊断。 此外，本发明涉及一种试剂盒，其包含用于敏感检测一种或多种靶核酸的ISH测定和一般ISH信号扩增测定的组分。

公开(公告)号：US09091691B2

公开(公告)日：2015-07-28

申请号：US13502727

申请日：2010-10-15

Applicant: Jesper Lohse

Inventor： Jesper Lohse

IPC: G01N33/535 ,  C12Q1/28 ,  G01N33/53 ,  G01N33/58 ,  G01N33/543

CPC classification number: G01N33/581 ,  G01N33/54306

Abstract: The present invention relates to immunochemical visualization and quantification of single target entities, such as single molecules, single molecular structures, single particles, etc. in samples wherein said single entities are immobilized. In particular, the invention relates to methods for visualization and quantification of single units of biological or chemical targets, in particular to immunochemical visualization of single molecules of biological targets in histological samples. The methods of the invention comprise a step of forming discrete deposits of detectable molecules at single target sites of sample mediated by an enzyme with oxydoreductase activity, wherein a single target site comprises a single unit of a target. The invention also relates to assays comprising the present visualization and quantification methods and diagnostic applications of said methods.

Abstract translation: 本发明涉及在其中所述单个实体被固定的样品中的单一目标实体（例如单分子，单分子结构，单个颗粒等）的免疫化学可视化和定量。 特别地，本发明涉及用于可视化和定量生物或化学靶单个单位的方法，特别是组织学样品中单分子生物靶标的免疫化学可视化。 本发明的方法包括在具有氧化还原酶活性的酶介导的样品的单个靶位点处形成可检测分子的离散沉积物的步骤，其中单个靶位点包含单个靶标单位。 本发明还涉及包括本发明的可视化和定量方法以及所述方法的诊断应用的测定。

公开(公告)号：US09045794B2

公开(公告)日：2015-06-02

申请号：US13984251

申请日：2012-02-07

Applicant: Ammon B. Peck ,  Cuong Q. Nguyen ,  Nigel G. John Richards

Inventor： Ammon B. Peck ,  Cuong Q. Nguyen ,  Nigel G. John Richards

IPC: C12Q1/26 ,  C12Q1/28 ,  C12Q1/527 ,  G01N33/52

CPC classification number: G01N33/523 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/527 ,  G01N33/84 ,  G01N2800/347

Abstract: The present invention features compositions and methods for the detection or measurement of oxalate in a sample. Such compositions include test devices that provide for the rapid and accurate detection of oxalate in a sample from a biological fluid. Advantageously, the compositions can be used to monitor the oxalate levels of a patient at a point of care (e.g., at the patient's home, clinic, physician's office, or other clinical setting).

Abstract translation: 本发明的特征在于用于检测或测量样品中草酸盐的组合物和方法。 这样的组合物包括提供来自生物流体的样品中草酸盐的快速和准确检测的测试装置。 有利地，组合物可以用于在护理点（例如，在患者的家，诊所，医师办公室或其他临床环境）监测患者的草酸盐水平。

公开(公告)号：US09040252B2

公开(公告)日：2015-05-26

申请号：US13167137

申请日：2011-06-23

Applicant: Leopoldo Della Ciana ,  Federica Rodeghiero ,  Rossana Perciaccante

Inventor： Leopoldo Della Ciana ,  Federica Rodeghiero ,  Rossana Perciaccante

IPC: G01N21/76 ,  G01N33/53 ,  G01N33/58 ,  C12Q1/28

CPC classification number: G01N33/582 ,  C12Q1/28 ,  G01N33/5306

Abstract: Method for increasing and regulating the emission of light from a chemiluminescent reaction including luminol, a peroxidase enzyme, an oxidant and an electron mediator (primary enhancer) through the use of an acylation catalyst (secondary enhancer) belonging to the class of N-azoles, i.e., a class of five-membered nitrogen heteroaromatic ring compounds containing at least one other atom of nitrogen. N-azoles, which are especially useful as secondary enhancers are imidazole, 1-methylimidazole, 1,2,3-triazole and 1,2,4-triazole. The invention also describes the use in diagnostic assays of chemiluminescent substrates containing said N-azoles, as secondary enhancers.

Abstract translation: 通过使用属于N-吡咯类的酰化催化剂（二级增强剂）来增加和调节包括鲁米诺，过氧化物酶，氧化剂和电子介体（初级增强剂）的化学发光反应的光的发射的方法， 即一类含有至少一个其他氮原子的五元氮杂芳族环化合物。 作为二次增效剂特别有用的N-吡咯是咪唑，1-甲基咪唑，1,2,3-三唑和1,2,4-三唑。 本发明还描述了在含有所述N-唑类的化学发光底物的诊断测定中用作次级增强剂的用途。

公开(公告)号：US09012170B2

公开(公告)日：2015-04-21

申请号：US14157281

申请日：2014-01-16

Applicant: Massachusetts Institute of Technology

Inventor： Alice Y. Ting ,  Jeffrey Daniel Martell

IPC: C12Q1/28 ,  G01N33/58 ,  C12N9/08

CPC classification number: C12N9/0065 ,  C12Q1/28 ,  G01N33/581

Abstract: An imaging method utilizing a split peroxidase is described herein. Imaging methods involve contacting a cell with a split peroxidase and a substrate thereof to allow conversion of a substrate into a product via an enzymatic reaction catalyzed by the reconstitute split peroxidase. Also disclosed herein are split peroxidases, related products and kits.

Abstract translation: 本文描述了利用裂解过氧化物酶的成像方法。 成像方法包括使细胞与裂解的过氧化物酶及其底物接触，以通过由重构的裂解过氧化物酶催化的酶反应将底物转化成产物。 本文还公开了分离的过氧化物酶，相关产物和试剂盒。

公开(公告)号：US09012169B2

公开(公告)日：2015-04-21

申请号：US11632977

申请日：2005-07-15

Applicant: Boris Schilling

Inventor： Boris Schilling

IPC: C07K14/705 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/44 ,  C12Q1/48

CPC classification number: C07K14/705 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/32 ,  C12Q1/34 ,  C12Q1/44 ,  C12Q1/48

Abstract: The invention is concerned with the identification of compounds as flavors or fragrances, their precursors, or as modulators of the perception of fragrances or flavors. The method comprises a reaction of a compound with a metabolic enzyme expressed in the nose, mouth or respiratory tract, followed by a method of identification of the compound or its metabolites as a fragrance or flavor, their precursor, or a modulator of their perception or of the perception of their respective leads.

Abstract translation: 本发明涉及将化合物鉴定为香料或香料，其前体，或作为香料或调味剂感觉的调节剂。 该方法包括化合物与在鼻，口或呼吸道中表达的代谢酶的反应，随后将化合物或其代谢物鉴定为香料或调味剂，其前体或其感知或调节剂的方法 对各自领导的看法。

公开(公告)号：US20150079620A1

公开(公告)日：2015-03-19

申请号：US14391885

申请日：2013-04-18

Applicant: KYOWA MEDEX CO., LTD.

Inventor： Hideyuki Kuwata ,  Tomoko Aratake ,  Kenta Kinjo

IPC: C12Q1/28

CPC classification number: C12Q1/28 ,  C09B21/00 ,  G01N33/581 ,  G01N33/92

Abstract: Provided is a measuring method of a component to be measured in a specimen while suppressing an influence of bilirubin. A measuring method of a component to be measured, comprising converting the component to be measured in the specimen to hydrogen peroxide through an enzymatic reaction, reacting the formed hydrogen peroxide with an oxidative-coloring chromogen in the presence of a peroxidase, and measuring an absorbance of the colored reaction solution to thereby determine the component to be measured, wherein a fatty acid or a salt thereof coexists. The measuring method of a component to be measured in a specimen according to the present invention is useful in clinical diagnosis.

Abstract translation: 提供了一种抑制胆红素的影响的试样中待测部件的测定方法。 1.一种被测定成分的测定方法，其特征在于，将通过酶反应将样品中待测量的成分转化为过氧化氢，在过氧化物酶的存在下使形成的过氧化氢与氧化着色色原体反应，并测定吸光度 的着色反应溶液，由此确定待测量的组分，其中脂肪酸或其盐共存。 根据本发明的样品中待测量的组分的测量方法可用于临床诊断。

公开(公告)号：US08927226B2

公开(公告)日：2015-01-06

申请号：US14266143

申请日：2014-04-30

Applicant: SurModics, Inc.

Inventor： Gary Opperman ,  Wendy Nelson

IPC: G01N33/58 ,  C12Q1/28 ,  G01N21/78 ,  G01N33/52 ,  G01N33/535 ,  G01N33/558

CPC classification number: G01N33/581 ,  C12Q1/28 ,  G01N21/78 ,  G01N33/52 ,  G01N33/535 ,  G01N33/558

Abstract: The invention provides compositions, kits, and methods for performing colorimetric analysis. A substrate is reacted to generate a chromogenic reaction product, and a reaction stop reagent that is a sulfonic acid is added to stop and stabilize the reaction product. The absorbance properties of the chromogenic reaction product can be maintained over significantly longer periods of time of that of conventional reagents and methods. The sulfonic acid can be used in assays such as ELISAs in order to provide a more accurate and safer detection of analytes in a biological sample.

Abstract translation: 本发明提供用于进行比色分析的组合物，试剂盒和方法。 使底物反应生成显色反应产物，并加入作为磺酸的反应停止试剂以停止并稳定反应产物。 显色反应产物的吸光度特性可以在常规试剂和方法的显着更长的时间内保持。 磺酸可用于诸如ELISA的测定中，以便提供对生物样品中分析物的更准确和更安全的检测。