公开(公告)号：US20200255851A1

公开(公告)日：2020-08-13

申请号：US16749576

申请日：2020-01-22

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Michelle Leslie

IPC: C12N15/82 ,  C12N9/64 ,  C12N9/24 ,  C07K14/32 ,  C07K14/415

Abstract: Isolated bioactive priming peptides and bioactive priming compostions comprising bioactive priming polypeptides and/or inducer compounds are provided that are useful when applied to plants in agricultural formulations. Methods of using the isolated bioactive priming peptides and/or compositions are also provided which are applied exogenously to the surface of a plant or a plant cell membrane or endogenously to the interior of a plant or to a plant cell. The isolated bioactive priming peptides and/or bioactive priming compositions when applied to a plant, a plant part, or a plant growth medium or a rhizosphere in an area surrounding the plant or the plant part increase growth, yield, health, longevity, productivity, and/or vigor of a plant or a plant part and/or protect the plant or the plant part from disease, and/or increase the innate immune response of the plant or the plant part and/or improve the quality and/or quantity of juice obtained from a plant or plant part.

公开(公告)号：US10717767B2

公开(公告)日：2020-07-21

申请号：US16041059

申请日：2018-07-20

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Michelle Leslie

IPC: C07K14/32 ,  C07K14/195 ,  C07K14/27 ,  C07K14/245 ,  C12P21/02 ,  A01N37/46 ,  A01N63/10 ,  C07K14/21

Abstract: Bioactive priming polypeptides are provided that are useful when applied to plants in agricultural formulations. Methods of using the formulations containing the bioactive priming polypeptides are also provided which are applied exogenously to the surface of a plant or a plant cell membrane or endogenously to the interior of a plant or to a plant cell. The bioactive priming polypeptides when applied to a plant, a plant part, or a plant growth medium or a rhizosphere in an area surrounding the plant or the plant part increase growth, yield, health, longevity, productivity, and/or vigor of a plant or a plant part and/or decrease abiotic stress in the plant or the plant part and/or protect the plant or the plant part from disease, insects and/or nematodes, and/or increase the innate immune response of the plant or the plant part and/or change plant architecture.

公开(公告)号：US20200170258A1

公开(公告)日：2020-06-04

申请号：US16631581

申请日：2018-07-20

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Michelle Leslie

IPC: A01N63/22 ,  C07K14/32 ,  C12N1/20 ,  A01N63/25 ,  A01N63/50 ,  C12P21/02

Abstract: Bioactive priming polypeptides are provided that are useful when applied to plants in agricultural formulations. Methods of using the formulations containing the bioactive priming polypeptides are also provided which are applied exogenously to the surface of a plant or a plant cell membrane or endogenously to the interior of a plant or to a plant cell. The bioactive priming polypeptides when applied to a plant, a plant part, or a plant growth medium or a rhizosphere in an area surrounding the plant or the plant part increase growth, yield, health, longevity, productivity, and/or vigor of a plant or a plant part and/or decrease abiotic stress in the plant or the plant part and/or protect the plant or the plant part from disease, insects and/or nematodes, and/or increase the innate immune response of the plant or the plant part and/or change plant architecture.

公开(公告)号：US10407472B2

公开(公告)日：2019-09-10

申请号：US15842062

申请日：2017-12-14

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Ashley Siegel

IPC: C07K14/32 ,  A01N37/44 ,  A61L2/18 ,  C09K8/62 ,  E21B43/16 ,  A23K20/147 ,  C12P21/02 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  A01N63/02 ,  C02F3/34 ,  A23K10/18 ,  A61K39/00 ,  C02F103/00 ,  C02F103/06 ,  C02F103/10 ,  C02F103/14 ,  C02F103/16 ,  C02F103/26 ,  C02F103/28 ,  C02F101/10 ,  C02F101/30

Abstract: Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

公开(公告)号：US20180364155A1

公开(公告)日：2018-12-20

申请号：US15982752

申请日：2018-05-17

Applicant: Spogen Biotech Inc. ,  Nufarm Limited

Inventor： Brian M. Thompson

IPC: G01N21/31 ,  G01J3/02 ,  G01N21/01 ,  G01N21/25 ,  G01N21/80

Abstract: Systems, devices, and methods for detecting agrochemicals in environments associated with agricultural equipment are described. Certain agrochemicals that are formulated for being detected using the systems, devices, and methods disclosed herein are also described. The devices, systems, and methods disclosed herein are generally configured to use spectral characteristics to detect agrochemicals in an environment associated with agricultural equipment. The spectral characteristics can be analyzed in various ways to provide different types of information about the agrochemicals and/or the environment.

公开(公告)号：US10092009B2

公开(公告)日：2018-10-09

申请号：US15414050

申请日：2017-01-24

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Katie Thompson

IPC: C12N15/62 ,  A01N63/00 ,  C12N9/02 ,  C12N9/42 ,  C12N9/48 ,  C12N9/20

Abstract: The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins, formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth and for protecting plants from pathogens by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plant roots.

公开(公告)号：US20160262402A1

公开(公告)日：2016-09-15

申请号：US14775858

申请日：2014-03-17

Applicant: SPOGEN BIOTECH INC.

Inventor： Brian Thompson ,  Katie Thompson ,  Brittany Angle

IPC: A01N63/02 ,  C12R1/05 ,  C12R1/125 ,  C12R1/07 ,  C12R1/01 ,  C12R1/085

CPC classification number: A01N63/00 ,  A01H3/00 ,  A01N63/02 ,  C12R1/01 ,  C12R1/05 ,  C12R1/07 ,  C12R1/075 ,  C12R1/085 ,  C12R1/125 ,  A01N25/00

Abstract: The present invention generally relates to bacteriologically pure bacterial cultures of novel strains of plant growth-promoting bacteria, and inoculums comprising the same. The invention is also directed to plant seeds coated with the inoculums, kits comprising the inoculums and methods for stimulating plant growth by applying the biologically pure bacterial culture or the inoculum to a plant, plant seed, or plant growth medium.

公开(公告)号：US20160108096A1

公开(公告)日：2016-04-21

申请号：US14857606

申请日：2015-09-17

Applicant: SPOGEN BIOTECH INC.

Inventor： Brian Thompson ,  Ashley Siegel

IPC: C07K14/32 ,  A23K1/16 ,  C12N9/18 ,  A01N63/00 ,  E21B43/16 ,  A61L2/18 ,  A23K1/00 ,  C09K8/62 ,  C02F3/34 ,  A61K39/07 ,  A01N37/44

CPC classification number: C07K14/32 ,  A01N37/44 ,  A01N63/02 ,  A23K10/18 ,  A23K20/147 ,  A61K39/00 ,  A61L2/18 ,  C02F3/342 ,  C02F3/348 ,  C02F2101/10 ,  C02F2101/30 ,  C02F2103/002 ,  C02F2103/003 ,  C02F2103/007 ,  C02F2103/06 ,  C02F2103/10 ,  C02F2103/14 ,  C02F2103/16 ,  C02F2103/26 ,  C02F2103/28 ,  C07K2319/01 ,  C07K2319/035 ,  C07K2319/40 ,  C09K8/62 ,  C09K2208/24 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C12P21/02 ,  E21B43/16

Abstract: Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

Abstract translation: 提供了含有将融合蛋白靶向蜡状芽孢杆菌家族成员的外源的靶向序列，外切蛋白或外切蛋白片段的融合蛋白。 还提供了表达这种融合蛋白的重组蜡状芽孢杆菌家族成员。 还提供遗传灭活的蜡样芽孢杆菌家族成员和过表达外胚蛋白的重组蜡状芽孢杆菌家族成员。 还提供了用重组蜡状芽孢杆菌家族成员包被的种子和使用重组蜡状芽孢杆菌家族成员的方法（例如刺激植物生长）。 还提供了表达融合蛋白的重组蜡状芽孢杆菌家族成员的各种修饰。 还提供了包含孢子外壳蛋白和感兴趣的蛋白质或肽的融合蛋白，表达这种融合蛋白的重组细菌，用这种重组细菌包被的种子，以及使用这种重组细菌（例如刺激植物生长）的方法。

公开(公告)号：US20240324599A1

公开(公告)日：2024-10-03

申请号：US18615771

申请日：2024-03-25

Applicant: Spogen Biotech Inc.

Inventor： Brian M. Thompson ,  Andrew M. Mutka ,  Alejandro Tovar-Mendez ,  Erica D. Mueller

IPC: A01N63/22 ,  A01N63/50 ,  A01P5/00 ,  A01P7/04 ,  A01P9/00 ,  A01P21/00 ,  C12N1/00 ,  C12N9/20 ,  C12N9/42 ,  C12N9/50

CPC classification number: A01N63/22 ,  A01N63/50 ,  A01P5/00 ,  A01P7/04 ,  A01P9/00 ,  A01P21/00 ,  C12N1/00 ,  C12N9/20 ,  C12N9/2442 ,  C12N9/50

Abstract: Isolated enzymes exhibiting insecticidal, nematicidal, or pesticidal activity, and recombinant microorganisms expressing said enzymes are provided as well as methods of using the same to protect a plant from a pathogen or pest, improve plant health, or improve plant growth are provided.

公开(公告)号：US20240316173A1

公开(公告)日：2024-09-26

申请号：US18654255

申请日：2024-05-03

Applicant: Spogen Biotech Inc. ,  Bayer CropScience LP

Inventor： Damian Curtis ,  Brian Thompson

IPC: A61K39/07 ,  A61K35/00 ,  A61K35/742

CPC classification number: A61K39/07 ,  A61K35/742 ,  A61K2035/115

Abstract: The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one particular fungicide disclosed herein in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and/or reducing overall damage of plants and plant parts.