公开(公告)号：US20240285745A1

公开(公告)日：2024-08-29

申请号：US18654243

申请日：2024-05-03

Applicant: Spogen Biotech Inc. ,  Bayer CropScience LP

Inventor： Damian Curtis ,  Brian Thompson

IPC: A61K39/07 ,  A61K35/00 ,  A61K35/742

CPC classification number: A61K39/07 ,  A61K35/742 ,  A61K2035/115

Abstract: The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one particular fungicide disclosed herein in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and/or reducing overall damage of plants and plant parts.

公开(公告)号：US20240285743A1

公开(公告)日：2024-08-29

申请号：US18644663

申请日：2024-04-24

Applicant: Spogen Biotech Inc. ,  Bayer CropScience LP

Inventor： Damian Curtis ,  Brian Thompson

IPC: A61K39/07 ,  A61K35/00 ,  A61K35/742

CPC classification number: A61K39/07 ,  A61K35/742 ,  C12Y301/01 ,  C12Y302/01004 ,  C12Y302/01132 ,  A61K2035/115 ,  Y02A50/30

Abstract: The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one particular insecticide disclosed herein in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and/or reducing overall damage of plants and plant parts.

公开(公告)号：US12016912B2

公开(公告)日：2024-06-25

申请号：US17356958

申请日：2021-06-24

Applicant: Bayer CropScience LP ,  Spogen Biotech Inc.

Inventor： Damian Curtis ,  Brian Thompson

IPC: A61K39/07 ,  A61K35/00 ,  A61K35/742

CPC classification number: A61K39/07 ,  A61K35/742 ,  C12Y301/01 ,  C12Y302/01004 ,  C12Y302/01132 ,  A61K2035/115 ,  Y02A50/30 ,  A01N63/50 ,  A01N63/22 ,  A01N63/50 ,  A01N43/22 ,  A01N43/56 ,  A01N43/90 ,  A01N47/22 ,  A01N47/40 ,  A01N53/00

Abstract: The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one particular insecticide disclosed herein in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and/or reducing overall damage of plants and plant parts.

公开(公告)号：US20240199709A1

公开(公告)日：2024-06-20

申请号：US18398650

申请日：2023-12-28

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Ashley Siegel

IPC: C07K14/32 ,  A01H3/00 ,  A01N37/44 ,  A01N63/10 ,  A01N63/20 ,  A01N63/50 ,  A23K10/18 ,  A23K20/147 ,  A61K39/00 ,  A61L2/18 ,  C02F3/34 ,  C02F101/10 ,  C02F101/30 ,  C02F103/00 ,  C02F103/06 ,  C02F103/10 ,  C02F103/14 ,  C02F103/16 ,  C02F103/26 ,  C02F103/28 ,  C09K8/62 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C12P21/02 ,  E21B43/16

CPC classification number: C07K14/32 ,  A01H3/00 ,  A01N37/44 ,  A01N63/10 ,  A01N63/50 ,  A23K20/147 ,  A61L2/18 ,  C02F3/342 ,  C02F3/348 ,  C09K8/62 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C12P21/02 ,  E21B43/16 ,  A01N63/20 ,  A23K10/18 ,  A61K39/00 ,  C02F2101/10 ,  C02F2101/30 ,  C02F2103/002 ,  C02F2103/003 ,  C02F2103/007 ,  C02F2103/06 ,  C02F2103/10 ,  C02F2103/14 ,  C02F2103/16 ,  C02F2103/26 ,  C02F2103/28 ,  C07K2319/01 ,  C07K2319/035 ,  C07K2319/40 ,  C09K2208/24

Abstract: Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

公开(公告)号：US20240132418A1

公开(公告)日：2024-04-25

申请号：US18476259

申请日：2023-09-26

Applicant: Spogen Biotech Inc.

Inventor： Brian M. Thompson ,  Jorg Augustin

IPC: C05F11/08 ,  A01N63/50 ,  C05B15/00 ,  C05C9/00 ,  C05F17/20 ,  C05G3/60 ,  C07K14/415 ,  C12N1/20 ,  C12N9/78 ,  C12N15/82

CPC classification number: C05F11/08 ,  A01N63/50 ,  C05B15/00 ,  C05C9/00 ,  C05F17/20 ,  C05G3/60 ,  C07K14/415 ,  C12N1/20 ,  C12N9/78 ,  C12N15/8237 ,  C12N15/8249 ,  C12N15/8279 ,  C12Y302/01 ,  C12Y305/99007 ,  C12Y402/0202 ,  Y02A40/22 ,  Y02P60/21

Abstract: Methods for stimulating plant growth and/or promoting plant health using free enzymes or recombinant microorganisms that overexpress enzymes are provided. Plant seeds coated with free enzymes or recombinant microorganisms that overexpress enzymes are also provided. Compositions comprising a fertilizer and an enzyme or a recombinant microorganism that overexpresses an enzyme are provided. Modified enzymes having ACC deaminase activity, recombinant microorganisms expressing the modified enzymes, plant seeds treated with the modified enzymes or recombinant microorganisms, and methods for stimulating plant growth and/or promoting plant health using the modified enzymes or recombinant microorganisms are also provided.

公开(公告)号：US20240132417A1

公开(公告)日：2024-04-25

申请号：US18476256

申请日：2023-09-26

Applicant: Spogen Biotech Inc.

Inventor： Brian M. Thompson ,  Jorg Augustin

IPC: C05F11/08 ,  A01N63/50 ,  C05B15/00 ,  C05C9/00 ,  C05F17/20 ,  C05G3/60 ,  C07K14/415 ,  C12N1/20 ,  C12N9/78 ,  C12N15/82

CPC classification number: C05F11/08 ,  A01N63/50 ,  C05B15/00 ,  C05C9/00 ,  C05F17/20 ,  C05G3/60 ,  C07K14/415 ,  C12N1/20 ,  C12N9/78 ,  C12N15/8237 ,  C12N15/8249 ,  C12N15/8279 ,  C12Y302/01 ,  C12Y305/99007 ,  C12Y402/0202 ,  Y02A40/22 ,  Y02P60/21

Abstract: Methods for stimulating plant growth and/or promoting plant health using free enzymes or recombinant microorganisms that overexpress enzymes are provided. Plant seeds coated with free enzymes or recombinant microorganisms that overexpress enzymes are also provided. Compositions comprising a fertilizer and an enzyme or a recombinant microorganism that overexpresses an enzyme are provided. Modified enzymes having ACC deaminase activity, recombinant microorganisms expressing the modified enzymes, plant seeds treated with the modified enzymes or recombinant microorganisms, and methods for stimulating plant growth and/or promoting plant health using the modified enzymes or recombinant microorganisms are also provided.

公开(公告)号：US20240109819A1

公开(公告)日：2024-04-04

申请号：US18476264

申请日：2023-09-27

Applicant: Spogen Biotech Inc.

Inventor： Brian M. Thompson ,  Jorg Augustin

IPC: C05F11/08 ,  A01N63/50 ,  C05B15/00 ,  C05C9/00 ,  C05F17/20 ,  C05G3/60 ,  C07K14/415 ,  C12N1/20 ,  C12N9/78 ,  C12N15/82

CPC classification number: C05F11/08 ,  A01N63/50 ,  C05B15/00 ,  C05C9/00 ,  C05F17/20 ,  C05G3/60 ,  C07K14/415 ,  C12N1/20 ,  C12N9/78 ,  C12N15/8237 ,  C12N15/8249 ,  C12N15/8279 ,  C12Y302/01 ,  C12Y305/99007 ,  C12Y402/0202 ,  Y02A40/22 ,  Y02P60/21

Abstract: Methods for stimulating plant growth and/or promoting plant health using free enzymes or recombinant microorganisms that overexpress enzymes are provided. Plant seeds coated with free enzymes or recombinant microorganisms that overexpress enzymes are also provided. Compositions comprising a fertilizer and an enzyme or a recombinant microorganism that overexpresses an enzyme are provided. Modified enzymes having ACC deaminase activity, recombinant microorganisms expressing the modified enzymes, plant seeds treated with the modified enzymes or recombinant microorganisms, and methods for stimulating plant growth and/or promoting plant health using the modified enzymes or recombinant microorganisms are also provided.

公开(公告)号：US11905315B2

公开(公告)日：2024-02-20

申请号：US17079942

申请日：2020-10-26

Applicant: Spogen Biotech Inc

Inventor： Brian Thompson ,  Ashley Siegel

IPC: C12N15/62 ,  A01H3/00 ,  A01N63/20 ,  C07K14/32 ,  A01N37/44 ,  A61L2/18 ,  C09K8/62 ,  E21B43/16 ,  C12P21/02 ,  C12N1/20 ,  C12N3/00 ,  C12N15/75 ,  C02F3/34 ,  A23K20/147 ,  A01N63/50 ,  A01N63/10 ,  A61K39/00 ,  C02F103/00 ,  C02F103/06 ,  C02F103/10 ,  C02F103/14 ,  C02F103/16 ,  C02F103/26 ,  C02F103/28 ,  A23K10/18 ,  C02F101/10 ,  C02F101/30

CPC classification number: C07K14/32 ,  A01H3/00 ,  A01N37/44 ,  A01N63/10 ,  A01N63/50 ,  A23K20/147 ,  A61L2/18 ,  C02F3/342 ,  C02F3/348 ,  C09K8/62 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C12P21/02 ,  E21B43/16 ,  A01N63/20 ,  A23K10/18 ,  A61K39/00 ,  C02F2101/10 ,  C02F2101/30 ,  C02F2103/002 ,  C02F2103/003 ,  C02F2103/007 ,  C02F2103/06 ,  C02F2103/10 ,  C02F2103/14 ,  C02F2103/16 ,  C02F2103/26 ,  C02F2103/28 ,  C07K2319/01 ,  C07K2319/035 ,  C07K2319/40 ,  C09K2208/24 ,  A01N63/50 ,  A01N63/22

Abstract: Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

公开(公告)号：US11898955B2

公开(公告)日：2024-02-13

申请号：US18049889

申请日：2022-10-26

Applicant: Spogen Biotech Inc.

Inventor： Brian M. Thompson

IPC: G01N21/31 ,  G01N21/3577 ,  G01N21/64 ,  G01N21/05 ,  G01J3/02 ,  G01N21/01 ,  G01N21/25 ,  G01N21/80 ,  G01N21/78 ,  G01N21/77 ,  G01N21/17

CPC classification number: G01N21/31 ,  G01J3/0218 ,  G01N21/01 ,  G01N21/05 ,  G01N21/255 ,  G01N21/80 ,  G01N21/3577 ,  G01N21/78 ,  G01N2021/0156 ,  G01N2021/1746 ,  G01N2021/6439 ,  G01N2021/7786

Abstract: Systems, devices, and methods for detecting agrochemicals in environments associated with agricultural equipment are described. Certain agrochemicals that are formulated for being detected using the systems, devices, and methods disclosed herein are also described. The devices, systems, and methods disclosed herein are generally configured to use spectral characteristics to detect agrochemicals in an environment associated with agricultural equipment. The spectral characteristics can be analyzed in various ways to provide different types of information about the agrochemicals and/or the environment.

公开(公告)号：US11882829B2

公开(公告)日：2024-01-30

申请号：US17025483

申请日：2020-09-18

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Katie Thompson

IPC: A01N63/00 ,  A01N37/46 ,  A01N63/50 ,  A01N63/22 ,  A01N63/10 ,  A61K38/16 ,  C07K14/32 ,  C12N9/02 ,  C12N9/16 ,  C12N9/24 ,  A01N37/48 ,  C12N9/20 ,  C12N9/42 ,  C12N9/48 ,  A61K39/07

CPC classification number: A01N37/46 ,  A01N37/48 ,  A01N63/00 ,  A01N63/10 ,  A01N63/22 ,  A01N63/50 ,  A61K38/164 ,  C07K14/32 ,  C12N9/0095 ,  C12N9/16 ,  C12N9/20 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/48 ,  C12Y118/06001 ,  C12Y301/01003 ,  C12Y302/01004 ,  C12Y304/00 ,  A61K39/07 ,  C07K2319/035 ,  C12Y301/03008 ,  A01N63/50 ,  A01N63/22 ,  A01N63/50 ,  A01N63/23

Abstract: The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins and formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth, for protecting plants from pathogens, and for enhancing stress resistance in a plant by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plants.