公开(公告)号：US20180231573A1

公开(公告)日：2018-08-16

申请号：US15510612

申请日：2015-09-11

Applicant: Beckman Coulter, Inc.

Inventor： Andreas VAN AGTHOVEN ,  Fabrice MALERGUE ,  Christophe GODEFROY ,  Enrique RABELLINO

IPC: G01N33/72 ,  G01N33/58 ,  G01N33/49 ,  G01N33/80 ,  G01N15/14

CPC classification number: G01N33/723 ,  G01N15/1459 ,  G01N33/492 ,  G01N33/582 ,  G01N33/721 ,  G01N33/726 ,  G01N33/80 ,  G01N2015/0076 ,  G01N2015/1402 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N2333/805

Abstract: Systems and methods are provided for determining the age of cellular hemoglobin in individual red blood cells in a blood sample by determining the percentage of HbA1c. In embodiments, a method includes measuring side scatter and fluorescence of the individual red blood cells and identifying immature red blood cells and mature red blood cells from the side scatter and fluorescence measurement. In embodiments, data collected includes the exact number of red blood cells, the fraction limits in fluorescence and side scatter units, the mean value of each fraction in fluorescence and side scatter units, the mean FL1 values per fraction in arbitrary units and the mean side scatter values per fraction in arbitrary units. In embodiments, a method also includes deriving a HbA1c content from the measured mean fluorescence of the individual red blood cells and determining the percentage of HbA1c from the HbA1c content and the hemoglobin content of the red blood cells.

公开(公告)号：US10048284B2

公开(公告)日：2018-08-14

申请号：US14699734

申请日：2015-04-29

Applicant: Beckman Coulter, Inc.

Inventor： Martin Müller ,  Andreas Weihs ,  Gerhard Gunzer ,  Michael Eberhardt

IPC: G01N35/00 ,  B65G47/28 ,  B01D21/26 ,  B04B7/08 ,  B04B15/00 ,  B25J11/00 ,  B04B9/14 ,  G01N35/04 ,  G01B11/02 ,  G01B11/08 ,  G01B11/10 ,  G01M1/14 ,  G01N21/27 ,  G01N35/10 ,  B04B7/02 ,  B04B13/00 ,  B65D51/24 ,  G01L19/08 ,  B04B11/04 ,  G01B11/24 ,  G01F23/00 ,  B01L3/00

Abstract: A sample container cap is disclosed. The sample cap container includes a sample cap body capable of covering an opening of a sample container and a centrifugation status indicator device in the top of the sample cap body. The centrifugation status indicator device includes: a first substance having a first density and a first color, and a second substance having a second density and a second color. The centrifugation status indicator device displays the first substance when the sample container has been centrifuged. The first substance can be a gel, and the second substance can include a plurality of particles. The second density is higher than the first density and the second color is different from the first color.

公开(公告)号：US20180188154A1

公开(公告)日：2018-07-05

申请号：US15714473

申请日：2017-09-25

Applicant: Beckman Coulter, Inc.

Inventor： Marshall Donnie Graham ,  William Gerry Graham ,  James P. Clarkin ,  Mark A. Wells ,  Jose M. Cano ,  Carlos Alberto Arboleda ,  Armando J. Sanchez

IPC: G01N21/05 ,  C03B23/047

CPC classification number: G01N21/05 ,  C03B23/047 ,  C03C27/00 ,  G01N15/1434 ,  G01N2015/1006 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2201/02 ,  Y02P40/57

Abstract: An improved optical flow cell adapted for use in a flow cytometer for differentiating formed bodies (e.g., blood cells) in liquid suspensions. Preferably manufactured by assembling, aligning, and optically joining at least two elements made from transparent material, the improved flow cell has a seamless internal flow channel of preferably non-circular cross-section in a cylindrical first element through which prepared samples can be metered and an independent second element having an external envelope suited to acquisition of optical parameters from formed bodies in such suspensions, the second element being conforming and alignable to the first element so that non-axisymmetric refractive effects on optical characterizing parameters of formed bodies passing through the flow channel in the first element may be minimized before the two elements are optically joined and fixed in working spatial relationship. Preferably, such first element is formed by a glass-drawing process in which a relatively large glass preform having a rectilinear internal channel of a desired cross-sectional shape is heated and drawn to achieve a desired cross-sectional area of reduced size. Preferably, such second element is provided by through-boring a suitable commercial optical component to conform to such first element. Also disclosed are preferred methods for differentiating formed bodies using the flow cell of the invention.

公开(公告)号：US20180180526A1

公开(公告)日：2018-06-28

申请号：US15829566

申请日：2017-12-01

Applicant: Beckman Coulter, Inc.

Inventor： George C. Malachowski ,  Paul Barclay Purcell ,  Edward Allan Stanton ,  Kenneth Michael Evans

IPC: G01N15/14 ,  G01N33/50 ,  G01N15/10 ,  G01N15/00

CPC classification number: G01N15/1429 ,  G01N15/1436 ,  G01N15/1468 ,  G01N15/1475 ,  G01N33/5005 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/149

Abstract: In flow cytometry, particles (2) can be distinguished between populations (8) by combining n-dimensional parameter data, which may be derived from signal data from a particle, to mathematically achieve numerical results representative of an alteration (48). An alteration may include a rotational alteration, a scaled alteration, or perhaps even a translational alteration. Alterations may enhance separation of data points which may provide real-time classification (49) of signal data corresponding to individual particles into one of at least two populations.

公开(公告)号：US20180172564A1

公开(公告)日：2018-06-21

申请号：US15899072

申请日：2018-02-19

Applicant: Beckman Coulter, Inc.

Inventor： Andreas Van Agthoven

IPC: G01N1/30

CPC classification number: G01N1/30

Abstract: The present invention relates to a cell treatment composition for the permeabilization of fixed blood cells, to the use of said composition, to a method for the treatment of a biological sample comprising fixation of said sample and subsequently contacting said biological sample with said cell treatment composition. The invention further relates and to a kit comprising said cell treatment composition.

公开(公告)号：US09903795B2

公开(公告)日：2018-02-27

申请号：US14139158

申请日：2013-12-23

Applicant: Beckman Coulter, Inc.

Inventor： Andreas Van Agthoven

IPC: G01N1/10 ,  G01N1/30

CPC classification number: G01N1/30

Abstract: The present invention relates to a cell treatment composition for the permeabilization of fixed blood cells, to the use of said composition, to a method for the treatment of a biological sample comprising fixation of said sample and subsequently contacting said biological sample with said cell treatment composition. The invention further relates and to a kit comprising said cell treatment composition.

公开(公告)号：US20180038829A1

公开(公告)日：2018-02-08

申请号：US15783388

申请日：2017-10-13

Applicant: Beckman Coulter, Inc.

Inventor： Brian D. Peterson ,  Dale Alan Owens ,  Peter S. Kotowski ,  Sunil S. Deliwala ,  Craig A. Hudson ,  James H. Reyes ,  Clive Cheung ,  Tom Tran ,  Anna X. Lou ,  Jane Luo ,  Jean-Marc Rene Lucien Busnel ,  Chitra K. Ratnayake

IPC: G01N27/447 ,  H01J49/02 ,  G01N30/72 ,  H01J49/16

CPC classification number: G01N27/44743 ,  G01N27/44717 ,  G01N30/7266 ,  H01J49/022 ,  H01J49/165 ,  Y10T307/313

Abstract: Aspects of the innovations presented herein relate to improved systems that in some embodiments perform capillary electrophoresis (CE) and CE in conjunction with electrospray ionization (ESI) as an input to a mass spectrometry system (MS). Some embodiments use a high voltage isolated CE power supply that is configured to float on the high voltage output of an ESI-MS power supply, with a protective resistance in the ESI-MS path, as well as DC/DC converter isolation and communication system isolation for the isolated CE power supply. Some embodiments additionally use a cartridge assembly integrating separation and conductive fluid capillaries with fluid cooling and protective retractable housings for the capillary end portions and for the ESI output. The protective housing may further be used with an adapter for interfacing with different MS systems.

公开(公告)号：US09816911B2

公开(公告)日：2017-11-14

申请号：US15036712

申请日：2014-11-14

Applicant: Beckman Coulter, Inc.

Inventor： Antao Chen ,  Juan J. Fernandez De Castro ,  Paul K. Church

IPC: G01N21/25 ,  G01N15/14 ,  G01N21/53 ,  G01N21/64 ,  G01N15/00

CPC classification number: G01N15/1436 ,  G01N15/1459 ,  G01N21/53 ,  G01N21/645 ,  G01N2015/0065 ,  G01N2021/6421 ,  G01N2021/6463 ,  G01N2021/6478 ,  G01N2021/6484 ,  G01N2201/0637

Abstract: High numerical aperture collection optics for particle analyzers may include an ellipsoidal reflector or an ellipsoidal reflector in combination with a spherical reflector, and may efficiently collect light scattered or emitted by particles in a sample stream and then couple that collected light into a lower numerical aperture portion of the instrument's optical detection system, such as into an optical fiber for example. The reflectors may be integrated with a flow cell through which the sample stream passes, or may be separate components arranged around a flow cell or, in instruments not employing a flow cell, arranged around a sample stream in air. Refractive beam steering optics may allow multiple closely spaced excitation beams to be directed into the sample stream at low angles of incidence. The collection optics and refractive beam steering optics may be employed separately or in combination with each other.

公开(公告)号：US20170297026A1

公开(公告)日：2017-10-19

申请号：US15457877

申请日：2017-03-13

Applicant: Beckman Coulter, Inc. ,  DTIL Ireland Holdings Limited

Inventor： Hiroyuki Onishi ,  Masato Kayahara ,  Hiroshi Suzuki ,  Ryusuke Furuya ,  Naoki Mukaiyama ,  Michael Cooney ,  Marcus O'Neill ,  Sean O'Mahony

IPC: B01L3/00 ,  B29C49/42 ,  G01N35/10 ,  B29C49/04 ,  B29L31/00

Abstract: An embodiment of a reagent container includes a bottle with a pipe to reduce the effects of reagent sloshing. The bottle has an elongated base and an opposed cover connected by side walls and an end wall. A flat platform surrounded by a raised rim lies in the base opposite an opening in the cover. A ribbed pipe frictionally fits within the bottle opening and may attach to the anchor region leaving vent passages around the pipe. The pipe includes an aperture adjacent to the anchor region and oriented toward the end wall so that sloshed fluid has only a small effect on the level of reagent in the pipe during transfers. A modified blow molding process produces the anchor region by extending a pin a predetermined distance into a mold while the molded material is still plastic.

公开(公告)号：US09772274B2

公开(公告)日：2017-09-26

申请号：US14770930

申请日：2014-03-14

Applicant: BECKMAN COULTER, INC.

Inventor： Marshall Donnie Graham ,  William Gerry Graham ,  James P. Clarkin ,  Mark A. Wells ,  Jose M. Cano ,  Carlos Alberto Arboleda ,  Armando J. Sanchez

IPC: G01N21/05 ,  C03B23/047 ,  C03C27/00 ,  G01N15/14 ,  G01N15/10

CPC classification number: G01N21/05 ,  C03B23/047 ,  C03C27/00 ,  G01N15/1434 ,  G01N2015/1006 ,  G01N2015/1452 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2201/02 ,  Y02P40/57

Abstract: An improved optical flow cell adapted for use in a flow cytometer for differentiating formed bodies (e.g., blood cells) in liquid suspensions. Preferably manufactured by assembling, aligning, and optically joining at least two elements made from transparent material, the improved flow cell has a seamless internal flow channel of preferably non-circular cross-section in a cylindrical first element through which prepared samples can be metered and an independent second element having an external envelope suited to acquisition of optical parameters from formed bodies in such suspensions, the second element being conforming and alignable to the first element so that non-axisymmetric refractive effects on optical characterizing parameters of formed bodies passing through the flow channel in the first element may be minimized before the two elements are optically joined and fixed in working spatial relationship.