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    BIOTINIDASE ASSAYS
    75.
    发明申请
    BIOTINIDASE ASSAYS 审中-公开
    生物测定测定

    公开(公告)号：US20150111237A1

    公开(公告)日：2015-04-23

    申请号：US14399051

    申请日：2013-05-07

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Carrie A. Graham ,  Allen E. Eckhardt

    IPC: C12Q1/34

    CPC classification number: C12Q1/34 ,  G01N33/542 ,  G01N33/582

    Abstract: The invention relates to methods for enzymatic detection of biotinidase activity. In certain aspects, the invention provides methods for bench-based enzymatic detection of biotinidase activity. In other aspects, the invention provides methods for bench-based enzymatic detection in droplets in oil. The droplet-based methods may be performed in an automated manner using digital microfluidics technology on a droplet actuator device. The enzymatic assays for biotinidase activity may be used for newborn testing for biotinidase deficiency, and may be combined with other droplet-based enzymatic assays in a panel of tests for newborn testing.

    Abstract translation: 本发明涉及酶检测生物素酶活性的方法。 在某些方面，本发明提供了用于基于酶的酶检测生物素酶活性的方法。 在其它方面，本发明提供了用于油中液滴的基于酶的酶检测方法。 基于液滴的方法可以使用液滴致动器装置上的数字微流体技术以自动方式进行。 用于生物素酶活性的酶测定可用于生物素酶缺陷的新生儿测试，并且可以在新生儿测试的一组测试中与其他基于液滴的酶测定组合。

    Techniques and Droplet Actuator Designs for Reducing Bubble Formation
    76.
    发明申请
    Techniques and Droplet Actuator Designs for Reducing Bubble Formation 有权
    技术和滴液致动器设计减少气泡形成

    公开(公告)号：US20150075986A1

    公开(公告)日：2015-03-19

    申请号：US14549113

    申请日：2014-11-20

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Delattre Cyril ,  Rival Arnaud ,  Srinivasan Vijay

    IPC: B01L3/02

    CPC classification number: B01L3/502784 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/5029 ,  B01L3/52 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/088 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0442 ,  B01L2400/0448

    Abstract: During droplet operations in a droplet actuator, bubbles often form in the filler fluid in the droplet operations gap and interrupt droplet operations. The present invention provides methods and systems for performing droplet operations on a droplet in a droplet actuator comprising maintaining substantially consistent contact between the droplet and an electrical ground while conducting multiple droplet operations on the droplet in the droplet operations gap and/or reducing the accumulation of electrical charges in the droplet operations gap during multiple droplet operations. The methods and systems reduce or eliminate bubble formation in the filler fluid of the droplet operations gap, thereby permitting completion of multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.

    Abstract translation: 在液滴致动器中的液滴操作期间，液滴操作间隙中的气泡通常在填充液中形成并且中断液滴操作。 本发明提供了用于在液滴致动器中的液滴上进行液滴操作的方法和系统，包括在液滴操作间隙内对液滴进行多个液滴操作并且/或减少 在多个液滴操作期间液滴操作间隙中的电荷。 所述方法和系统减少或消除了液滴操作间隙的填充流体中的气泡形成，从而允许完成多个液滴操作，而不会由于液滴操作间隙中的填充流体中的气泡形成而中断。

    DROPLET OPERATIONS DEVICE
    77.
    发明申请
    DROPLET OPERATIONS DEVICE 审中-公开
    DROPLET操作装置

    公开(公告)号：US20150068903A1

    公开(公告)日：2015-03-12

    申请号：US14498418

    申请日：2014-09-26

    Applicant: Advanced Liquid Logic, Inc.

    Inventor： Vijay Srinivasan ,  Arjun Sudarsan ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Ramakrishna Sista ,  Raymond Kozikowski ,  Nicole Bell

    IPC: G01N27/447

    CPC classification number: G01N27/44756 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502707 ,  B01L3/502792 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2400/0427 ,  B01L2400/0688 ,  B01L2400/086 ,  B29C59/02 ,  B41J2/1601 ,  B81B7/0061 ,  B81B2201/058 ,  B81B2201/13 ,  F04B17/00 ,  F04B19/006

    Abstract: The invention provides droplet actuators with droplet operations surfaces for manipulating droplets, e.g., by conducting droplet operations. The droplet operations surfaces are typically exposed to a droplet operations gap. One or more regions of a droplet operation surface may include patterned topographic features. The invention also provides a droplet actuator in which one or both gap-facing droplet operations surfaces is formed using a removable film. The removable film may, in various embodiments, also include other components ordinarily associated with the droplet actuator substrate, such as the dielectric layer and the electrodes. Further, the invention provides droplet actuator devices and methods for coupling and/or sealing substrates of a droplet actuator, such as techniques for self-aligning assembly of droplet actuator substrates. The invention provides droplet actuators and methods of disassembling the droplet actuator in order to provide access for cleaning and/or recycling of droplet actuator surfaces.

    Abstract translation: 本发明提供了液滴致动器，其具有用于操纵液滴的液滴操作表面，例如通过进行液滴操作。 液滴操作表面通常暴露于液滴操作间隙。 液滴操作表面的一个或多个区域可以包括图案化的地形特征。 本发明还提供一种液滴致动器，其中使用可去除的膜形成一个或两个面向间隙的液滴操作表面。 在各种实施例中，可除去膜可以包括通常与液滴致动器基底（例如电介质层和电极）相关联的其它部件。 此外，本发明提供了液滴致动器装置和用于联接和/或密封液滴致动器的基板的方法，例如用于液滴致动器基板的自对准组装的技术。 本发明提供了液滴致动器和拆卸液滴致动器的方法，以便为液滴致动器表面的清洁和/或回收提供通路。

    Method of reducing liquid volume surrounding beads
    79.
    发明授权
    Method of reducing liquid volume surrounding beads 有权
    降低珠粒周围液体的方法

    公开(公告)号：US08927296B2

    公开(公告)日：2015-01-06

    申请号：US12992939

    申请日：2009-05-18

    Applicant: Ramakrishna Sista ,  Vamsee Pamula ,  Arjun Sudarsan ,  Vijay Srinivasan ,  Prasanna Thwar

    Inventor： Ramakrishna Sista ,  Vamsee Pamula ,  Arjun Sudarsan ,  Vijay Srinivasan ,  Prasanna Thwar

    IPC: G01N1/40 ,  B01L3/00 ,  G01N35/00

    CPC classification number: B01L3/502792 ,  B01L3/502761 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/089 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0487 ,  G01N1/4077 ,  G01N35/0098 ,  G01N2035/00564 ,  Y10T436/25

    Abstract: The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads.

    Abstract translation: 本发明提供了液滴致动器和液滴致动器技术。 除其他之外，液滴致动器和方法可用于操纵液滴致动器上的珠，例如使用液滴致动器上的含珠液滴进行液滴操作。 例如，在执行样品制备方案和/或测定方案的上下文中，珠可以在液滴致动器上操作。 本发明方法的输出可以是准备用于执行测定方案的珠粒。 本发明方法的另一个输出可以是使用珠子执行的测定方案的结果。 在本文描述的方法中，将珠粒集中在液滴中的方法，洗涤珠粒的方法，悬浮珠粒的方法，分离珠粒的方法，在液滴内定位珠粒的方法，形成液滴包括珠粒的乳液的方法，加载珠粒的方法 液滴致动器的液滴操作间隙，单层组织珠粒的方法，以及捕获，捕获或抑制珠粒的方法。

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