公开(公告)号：US20120058917A1

公开(公告)日：2012-03-08

申请号：US12593770

申请日：2008-04-04

Applicant: Johannes Adrianus Gaken ,  Azim Mohamedali

Inventor： Johannes Adrianus Gaken ,  Azim Mohamedali

IPC: C40B30/06 ,  C12N5/10 ,  C40B50/00 ,  C12Q1/68 ,  C12N15/63 ,  C40B40/02

CPC classification number: B29C39/10 ,  H01B3/006 ,  H01G4/206 ,  H05K1/024 ,  H05K1/0373 ,  H05K1/162 ,  H05K2201/0116 ,  H05K2201/0209 ,  H05K2201/0218 ,  H05K2201/0254 ,  Y10T428/24967

Abstract: The invention relates to a nucleic acid comprising the following contiguous elements arranged in the 5 prime to 3 prime direction; a promoter; a selectable marker; a cloning site for receipt of a nucleic acid segment, said segment comprising a candidate miRNA target sequence; and a poly adenylation signal, said elements arranged such that a transcript directed by said promoter comprises said selectable marker, said candidate miRNA target sequence, and said poly adenylation signal in that order. Suitably the miRNA test sequence is or is derived from a 3′UTR. The invention also relates to methods for making and screening libraries.

Abstract translation: 本发明涉及一种核酸，其包含排列成5素质素至3个原子方向的以下邻接元件; 启动子 可选标记; 用于接收核酸片段的克隆位点，所述片段包含候选miRNA靶序列; 和聚腺苷酸化信号，所述元件排列成使得由所述启动子引导的转录物依次包含所述选择性标记，所述候选miRNA靶序列和所述多聚腺苷酸化信号。 适当地，miRNA测试序列是或源自3'UTR。 本发明还涉及制备和筛选文库的方法。