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    Automated cell processing systems and methods
    2.
    发明授权
    Automated cell processing systems and methods 有权

    公开(公告)号：US11125767B2

    公开(公告)日：2021-09-21

    申请号：US16676328

    申请日：2019-11-06

    Applicant: Genesis Technologies Limited

    Inventor： Jan-Eric Ahlfors

    IPC: G01N35/10 ,  B01L1/02 ,  B01L1/04 ,  G01N35/00

    Abstract: A system for automated processing of a plurality of batches, each batch being derived from one biological sample, the system comprising an enclosure which can be closed and sterilized, each batch of the plurality of batches comprising one or more cell processing container; a plurality of reagent containers for holding reagents within the enclosure; at least one reagent dispenser within the enclosure for dispensing reagents during said automated processing; a quality control system within the enclosure for analyzing at least one characteristic of a batch during said automated processing; a harvester within the enclosure for harvesting batches; a robotic system within the enclosure, configured for transporting cell processing containers, decapping or otherwise opening cell processing containers, pipetting reagents or liquids from cell processing containers, and aspirating liquids from cell processing containers, during said automated processing; a tracker for electronically tracking the plurality of batches after its introduction to the enclosure; and a control unit (CU) communicatively coupled to the at least one reagent dispenser, the quality control system, the harvester, the robotic system and the tracker for controlling said automatic processing of said batches.

    METHODS FOR REPROGRAMMING CELLS AND USES THEREOF
    3.
    发明申请
    METHODS FOR REPROGRAMMING CELLS AND USES THEREOF 审中-公开

    公开(公告)号：US20190085294A1

    公开(公告)日：2019-03-21

    申请号：US16195005

    申请日：2018-11-19

    Applicant: Genesis Technologies Limited

    Inventor： Jan-Eric Ahlfors ,  Rouwayda El-Ayoubi

    IPC: C12N5/0775 ,  C12N5/0797 ,  C12N5/079 ,  C12N5/077 ,  C12N5/0793 ,  C12N5/0789 ,  C12N5/074

    Abstract: A method of obtaining a neural multipotent, unipotent or somatic cell, including: i) providing a cell of a first type which is not a neural multipotent, unipotent or somatic cell; ii) increasing expression of at least one neural multipotent or unipotent gene regulator in the cell of a first type, to a level at which the at least one neural multipotent or unipotent gene regulator is capable of driving transformation of the cell of a first type into the neural multipotent, unipotent or somatic cell, wherein the at least one multipotent or unipotent gene regulator is Musashi1 (Msi1), Neurogenin 2 (Ngn2), or both Msi1 and Ngn2; and iii) placing or maintaining the cell in a neural cell culture medium and maintaining sufficient intracellular levels of the at least one multipotent or unipotent gene regulator for a sufficient period of time to allow a stable neural multipotent, unipotent or somatic cell to be obtained.

    Methods for reprogramming cells and uses thereof
    5.
    发明授权
    Methods for reprogramming cells and uses thereof 有权

    公开(公告)号：US10131879B2

    公开(公告)日：2018-11-20

    申请号：US15298006

    申请日：2016-10-19

    Applicant: GENESIS TECHNOLOGIES LIMITED

    Inventor： Jan-Eric Ahlfors ,  Rouwayda El-Ayoubi

    IPC: C12N5/00 ,  C12N5/0775 ,  C12N5/0797 ,  C12N5/079 ,  C12N5/074 ,  A61K35/30 ,  C12N5/0789 ,  C12N5/077 ,  C12N5/0793 ,  C12N5/071 ,  A61K35/545

    Abstract: An in vitro human neural multipotent, unipotent, or somatic cell possessing all of the following characteristics: is derived from the reprogramming of a somatic cell, a progenitor cell or a stem cell that exhibits at least a transient increase in intracellular levels of at least one reprogramming agent; is not differentiated from a pluripotent cell; expresses one or more markers of a multipotent, unipotent or somatic cell not characteristic of a neural stem cell, neural precursor cell, neural progenitor cell, neuroblast, or neuron; is not a cancerous cell; is stable and not artificially maintained by forced gene expression and may be maintained in standard neural stem cell media or neural media; and does not exhibit uncontrolled growth, teratoma formation, and tumor formation in vivo; wherein the cell comprises at least one polypeptide or an expression vector encoding at least one polypeptide selected from the group consisting of: Musashi1 (Msi1); Ngn2; Msi1 and Ngn2; Msi1 and methyl-CpG binding domain protein 2 (MBD2); Ngn2 and MBD2; Msi1, Ngn2 and MBD2; Achaete-Scute Homolog 1 (Ascl1); Msi1, Ngn2 and Ascl1; Msi1, Ngn2, MBD2 and Ascl1; Sox2; Msi1, Ngn2 and Sox2; and Msi1, Ngn2, MBD2 and Sox2; wherein the expression vector is transiently expressed.

    AUTOMATED CELL PROCESSING SYSTEMS AND METHODS
    8.
    发明申请
    AUTOMATED CELL PROCESSING SYSTEMS AND METHODS 有权

    公开(公告)号：US20220003796A1

    公开(公告)日：2022-01-06

    申请号：US17448011

    申请日：2021-09-17

    Applicant: Genesis Technologies Limited

    Inventor： Jan-Eric Ahlfors

    IPC: G01N35/10 ,  B01L1/02 ,  B01L1/04 ,  G01N35/00

    Abstract: A system for automated processing of a plurality of batches, each batch being derived from one biological sample, the system comprising an enclosure which can be closed and sterilized, each batch of the plurality of batches comprising one or more cell processing container; a plurality of reagent containers for holding reagents within the enclosure; at least one reagent dispenser within the enclosure for dispensing reagents during said automated processing; a quality control system within the enclosure for analyzing at least one characteristic of a batch during said automated processing; a harvester within the enclosure for harvesting batches; a robotic system within the enclosure, configured for transporting cell processing containers, decapping or otherwise opening cell processing containers, pipetting reagents or liquids from cell processing containers, and aspirating liquids from cell processing containers, during said automated processing; a tracker for electronically tracking the plurality of batches after its introduction to the enclosure; and a control unit (CU) communicatively coupled to the at least one reagent dispenser, the quality control system, the harvester, the robotic system and the tracker for controlling said automatic processing of said batches.

    Automated cell processing systems and methods
    9.
    发明授权
    Automated cell processing systems and methods 有权

    公开(公告)号：US11156627B2

    公开(公告)日：2021-10-26

    申请号：US16311345

    申请日：2017-06-20

    Applicant: Genesis Technologies Limited

    Inventor： Jan-Eric Ahlfors

    IPC: G01N35/10 ,  B01L1/02 ,  B01L1/04 ,  G01N35/00

    Abstract: Systems and methods for automated cell processing of biological samples, such as cells for use in cell therapy and regenerative medicine. Systems for automated processing of batches derived from biological samples comprise: a closed and sterile enclosure; a plurality of reagent containers; at least one reagent dispenser; a quality control module for analyzing at least one characteristic of a batch; a harvesting module; a robotic module; and a control unit (CU) communicatively coupled to the at least one reagent dispenser, the quality control module, the harvesting module and the robotic module for controlling the automatic processing of batches. The automatic processing may be executable without handling by a human operator. The system may be configured to automatically process the plurality of batches without cross-contamination between batches, e.g., under GMP conditions.

    METHODS FOR REPROGRAMMING CELLS AND USES THEREOF
    10.
    发明申请
    METHODS FOR REPROGRAMMING CELLS AND USES THEREOF 审中-公开

    公开(公告)号：US20190024056A1

    公开(公告)日：2019-01-24

    申请号：US16125528

    申请日：2018-09-07

    Applicant: Genesis Technologies Limited

    Inventor： Jan-Eric Ahlfors ,  Rouwayda El-Ayoubi

    IPC: C12N5/074 ,  C12N15/85 ,  C12N5/0797 ,  C12N5/071 ,  C12N5/0775

    Abstract: Described herein are reprogrammed cells, and methods for cell dedifferentiation, transformation and eukaryotic cell reprogramming. Also described are cells, cell lines, and tissues that can be transplanted in a patient after steps of in vitro dedifferentiation and in vitro reprogramming. In particular embodiments the cells are Stem-Like Cells (SLCs), including Neural Stem-Like Cells (NSLCs), Cardiac Stem-Like Cells (CSLC), Hematopoietic Stem-Like Cells (HSLC), Pancreatic Progenitor-Like Cells, and Mesendoderm-like Cells. Also described are methods for generating these cells from human somatic cells and other types of cells. Also provided are compositions and methods of using of the cells so generated in human therapy and in other areas.

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