公开(公告)号：US12264352B2

公开(公告)日：2025-04-01

申请号：US17366950

申请日：2021-07-02

Applicant: Research Foundation of the City University of New York ,  Japanese Foundation for Cancer Research

Inventor： Hiroshi Matsui ,  Kiyotaka Shiba ,  Min A. Kang

IPC: C12P21/00 ,  C12N5/00

Abstract: A method for enhancing extracellular vesicle production is described. A peptide that induces polymer formation is incubated with a cell culture which results in enhanced EV production. The peptide penetrates the cells and subsequently polymerizes upon exposure to enzymes (e.g. phosphatase) within the cell. The cells that contain the newly formed polymers have an increased production of EVs. These EVs can be harvested using centrifugation techniques.

公开(公告)号：US20250099461A1

公开(公告)日：2025-03-27

申请号：US18263947

申请日：2022-02-10

Applicant: JAPANESE FOUNDATION FOR CANCER RESEARCH

Inventor： Ryohei Katayama

IPC: A61K31/497 ,  C12Q1/6886

Abstract: Tumors having acquired resistance to ALK-TKIs were analyzed by use of various kinase inhibitors. As a result, it was found that gilteritinib, which is a therapeutic drug for FLT3 gene mutation-positive acute myeloid leukemia, has an effect on the tumors. Gilteritinib directly inhibits the kinase activity of ALK to have an effect on an ALK fusion gene mutation-positive tumor having a plurality of compound mutations for which effective therapeutic drugs have been not present. ALK-TKI-resistant cancer developed via a fusion gene such as NTRK, ROS1 and LTK, and via AXL can be effectively overcome by gilteritinib alone, and the resistance mediated through ALK-TKI resistance mechanism via a bypass pathway such as KRAS, BRAF and EGFR, can be overcome by combination therapy.

公开(公告)号：US11566017B2

公开(公告)日：2023-01-31

申请号：US16958066

申请日：2018-12-26

Applicant: RIKEN ,  JAPANESE FOUNDATION FOR CANCER RESEARCH ,  KABUSHIKI KAISHA YAKULT HONSHA

Inventor： Minoru Yoshida ,  Hiroyuki Seimiya ,  Yoko Yashiroda ,  Kenichi Washizuka ,  Fumiyuki Shirai ,  Nobuko Yoshimoto ,  Junichi Kazami

IPC: C07D403/04 ,  C07D498/06

Abstract: Provided is a cell proliferation inhibitor comprising a compound of the formula (1) or a pharmacologically acceptable salt thereof: wherein J1 and J2 each represent CH or N, with the proviso that J1 and J2 are not simultaneously CH; r represents 0 to 4; each R101 is the same or different when r is 2 or more, and R101 represents a C1-6 alkyl group optionally substituted with a halogen atom or the like; s represents 0 to 5; each R102 is the same or different when s is 2 or more, and R102 represents a halogen atom or the like; R103 represents a hydrogen atom, a C1-6 alkyl group, a C3-6 cycloalkyl group, or a C3-6 cycloalkyl C1-6 alkyl group; and R101 and R103 are optionally linked together to form a five- to seven-membered ring hetero ring when R101 is present at the 8-position.

公开(公告)号：US20220291217A1

公开(公告)日：2022-09-15

申请号：US17639979

申请日：2020-09-04

Applicant: JAPANESE FOUNDATION FOR CANCER RESEARCH ,  TOSOH CORPORATION

Inventor： Koji Ueda ,  Naomi Ohnishi

IPC: G01N33/574

Abstract: Exosomes were purified from the sera of patients with gastric cancer and healthy subjects by using size-exclusion chromatography, and novel markers were obtained through mass spectrometry. In the patients with gastric cancer, 40 proteins with enhanced expression and 4 proteins with decreased expression can be suitable markers for detecting gastric cancer. Particularly, the detailed analysis of CA1, including its function, showed that gastric cancer could be detected with high sensitivity.

公开(公告)号：US11340215B2

公开(公告)日：2022-05-24

申请号：US16079895

申请日：2017-02-27

Applicant: JAPANESE FOUNDATION FOR CANCER RESEARCH

Inventor： Toru Hirota ,  Yusuke Abe

IPC: G01N33/50 ,  C07K16/18 ,  G01N33/574 ,  G01N33/68 ,  C12N15/113

Abstract: The inventors have found that the interaction between HP1 and INCENP can serve as an indicator for chromosome instability and established a method for evaluating chromosome instability of cancer cells. The evaluation system can be used for screening of anticancer agent with a new-concept of targeting chromosome instability of cancer cells. The inventors further prepared an antibody for specifically recognizing phosphorylation of serine at position 92 of HP1α, by which the action of Aurora B can be evaluated. The interaction between HP1 and INCENP can be readily evaluated by the antibody.

公开(公告)号：US20210403555A1

公开(公告)日：2021-12-30

申请号：US16626162

申请日：2019-06-14

Applicant: JAPANESE FOUNDATION FOR CANCER RESEARCH ,  API CO., LTD.

Inventor： Nobuhiko GYOBU ,  Naoya FUJITA ,  Mamoru KAKINO ,  Ai KAWASHIMA ,  Shinya FUJIHARA ,  Naoki GODA

IPC: C07K16/28 ,  C07K16/46

Abstract: The object of the present invention is to provide a humanized or mouse-human chimeric anti-podoplanin antibody or an antibody fragment containing the antigen-binding region thereof, and the object is achieved by providing an isolated humanized or mouse-human chimeric anti-podoplanin antibody which comprises a predetermined amino acid sequence, or an antibody fragment containing the antigen-binding region thereof.

公开(公告)号：US10921311B2

公开(公告)日：2021-02-16

申请号：US16070110

申请日：2017-01-13

Applicant: JAPANESE FOUNDATION FOR CANCER RESEARCH

Inventor： Kengo Takeuchi ,  Seiji Sakata ,  Yuki Togashi ,  Naoya Fujita ,  Ryohei Katayama

IPC: C07H21/04 ,  C12Q1/68 ,  G01N33/50 ,  C12N9/12 ,  G01N33/574 ,  C12N9/88 ,  C07K14/82 ,  A61K31/519 ,  A61P35/00 ,  A61K38/00 ,  C12N15/113 ,  C12N15/62 ,  C12Q1/6886 ,  C12Q1/6853 ,  C12Q1/686

Abstract: It is intended to reveal a polynucleotide serving as a novel causative gene of a cancer and, on the basis of this finding, to provide a method for detecting the polynucleotide or a polypeptide encoded thereby, a kit and a primer set for the detection, a method for screening for a substance that inhibits the polypeptide, and a pharmaceutical composition for the treatment of a cancer, containing the inhibiting substance. The detection method of the present invention detects a BRAF fusion protein or a fusion gene encoding the fusion protein, or a PXN or GMDS fusion protein or a fusion gene encoding the fusion protein in a digestive organ-derived sample obtained from a subject.

公开(公告)号：US20200337537A1

公开(公告)日：2020-10-29

申请号：US16760458

申请日：2018-10-30

Applicant: JAPANESE FOUNDATION FOR CANCER RESEARCH ,  Al Medical Service Inc.

Inventor： Toshiaki HIRASAWA ,  Tomohiro TADA ,  Kazuharu AOYAMA ,  Tsuyoshi OZAWA ,  Toshiyuki YOSHIO

IPC: A61B1/045 ,  A61B1/273 ,  A61B1/31 ,  G06K9/00

Abstract: Provided are: an image diagnosis assistance apparatus capable of assisting diagnosis of an endoscopic image captured by an endoscopist; a data collection method; an image diagnosis assistance method; and an image diagnosis assistance program. The image diagnosis assistance apparatus is provided with: a lesion assessment unit that assesses, by a convolutional neural network, the denomination and the position of a lesion which is present in a digestive system endoscopic image of a patient captured by a digestive system endoscopic imaging device and information about accuracies thereof; and a display control unit that performs control for generating an analysis result image in which the denomination and the position of the lesion and the accuracies thereof are displayed and for displaying the image on the digestive system endoscopic image.

公开(公告)号：US20200172874A1

公开(公告)日：2020-06-04

申请号：US16782579

申请日：2020-02-05

Applicant: TOPPAN PRINTING CO., LTD. ,  Japanese Foundation For Cancer Research

Inventor： Yuki TAKAHASHI ,  Shiro KITANO ,  Ryohei KATAYAMA ,  Satoshi NAGAYAMA

IPC: C12N5/09

Abstract: A primary culture method in which cells contained in a tissue collected from a living body are primary cultured in vitro, in which the cells in the tissue collected from the living body are seeded and cultured on a top surface of a cell structure containing cells constituting a stroma and composed of a single layer or two or more cell layers laminated in the thickness direction.

公开(公告)号：US20200025750A1

公开(公告)日：2020-01-23

申请号：US16303798

申请日：2017-05-23

Applicant: JAPANESE FOUNDATION FOR CANCER RESEARCH ,  NOF CORPORATION

Inventor： Kiyotaka Shiba ,  Nobuyuki Sakamoto ,  Tomozumi Noda ,  Satoshi Yamada

IPC: G01N33/543 ,  C08F230/02 ,  C09D143/02 ,  C08F20/28 ,  C09D133/14

Abstract: A coating agent for preventing the adsorption of extracellular vesicles represented by exosomes to a tool has been developed. Adsorption of extracellular vesicles to a tool can be prevented by using a coating agent which contains a hydrophilic polymer having a weight average molecular weight of 10,000 or more and 1,000,000 or less, wherein a coated layer formed by the coating agent has a contact angle of 0 degree or more and 30 degrees or less.