公开(公告)号：US08525980B2

公开(公告)日：2013-09-03

申请号：US13255911

申请日：2010-06-21

Applicant: Juqiang Lin ,  Rong Chen ,  Shangyuan Feng ,  Yongzeng Li ,  Guannan Chen ,  Zufang Huang ,  Min Cheng ,  Yun Yu ,  Haishan Zeng

Inventor： Juqiang Lin ,  Rong Chen ,  Shangyuan Feng ,  Yongzeng Li ,  Guannan Chen ,  Zufang Huang ,  Min Cheng ,  Yun Yu ,  Haishan Zeng

IPC: G01N1/00 ,  G01N21/00

CPC classification number: G01N1/00 ,  G01N21/00 ,  G01N21/658 ,  G01N27/447

Abstract: This invention provides a body fluid detection method by using surface enhanced Raman spectroscopy. In this method, some biological macromolecules in body fluid samples could be separated with membrane electrophoresis technique firstly. Next, samples are cut off along with the substrates and touched with glacial acetic acid. Transparent colloid formed while incubating. Then add enhancing substrates and continue to incubate and stir. When solid impurities precipitated, stop incubating and stand for layering. In the end, take upper layer resulted to be tested using SERS detection method and build SERS database. This invention successfully eliminated disturbance of other complex components on the SERS detection of protein, DNA and RNA. High quality SERS spectrum obtained is beneficial to the analysis and process of SERS spectrum. Thus body fluid can be differentiated by comparing body fluid SERS spectrum belonging to the healthy people and patients.

Abstract translation: 本发明通过使用表面增强拉曼光谱法提供体液检测方法。 在这种方法中，体液样品中的一些生物大分子首先用膜电泳技术分离。 接下来，将样品与基底一起切割并与冰醋酸接触。 孵化时形成透明胶体。 然后加入增强底物，继续孵育和搅拌。 当固体杂质沉淀时，停止孵育并代替分层。 最后，使用SERS检测方法测试上层，构建SERS数据库。 本发明成功地消除了其他复合成分对蛋白质，DNA和RNA的SERS检测的影响。 获得的高质量SERS光谱有利于SERS光谱的分析和处理。 因此，通过比较属于健康人和患者的体液SERS谱可以区分体液。

公开(公告)号：US20120062885A1

公开(公告)日：2012-03-15

申请号：US13255911

申请日：2010-06-21

Applicant: Juqiang Lin ,  Rong Chen ,  Shangyuan Feng ,  Yongzeng Li ,  Guannan Chen ,  Zufang Huang ,  Min Cheng ,  Yun Yu ,  Haishan Zeng

Inventor： Juqiang Lin ,  Rong Chen ,  Shangyuan Feng ,  Yongzeng Li ,  Guannan Chen ,  Zufang Huang ,  Min Cheng ,  Yun Yu ,  Haishan Zeng

IPC: G01J3/44

CPC classification number: G01N1/00 ,  G01N21/00 ,  G01N21/658 ,  G01N27/447

Abstract: This invention provides a body fluid detection method by using surface enhanced Raman spectroscopy. In this method, some biological macromolecules in body fluid samples could be separated with membrane electrophoresis technique firstly. Next, samples are cut off along with the substrates and touched with glacial acetic acid. Transparent colloid formed while incubating. Then add enhancing substrates and continue to incubate and stir. When solid impurities precipitated, stop incubating and stand for layering. In the end, take upper layer resulted to be tested using SERS detection method and build SERS database. This invention successfully eliminated disturbance of other complex components on the SERS detection of protein, DNA and RNA. High quality SERS spectrum obtained is beneficial to the analysis and process of SERS spectrum. Thus body fluid can be differentiated by comparing body fluid SERS spectrum belonging to the healthy people and patients.

Abstract translation: 本发明通过使用表面增强拉曼光谱法提供体液检测方法。 在这种方法中，体液样品中的一些生物大分子首先用膜电泳技术分离。 接下来，将样品与基底一起切割并与冰醋酸接触。 孵化时形成透明胶体。 然后加入增强底物，继续孵育和搅拌。 当固体杂质沉淀时，停止孵育并代替分层。 最后，使用SERS检测方法测试上层，构建SERS数据库。 本发明成功地消除了其他复合成分对蛋白质，DNA和RNA的SERS检测的影响。 获得的高质量SERS光谱有利于SERS光谱的分析和处理。 因此，通过比较属于健康人和患者的体液SERS谱可以区分体液。