公开(公告)号：US20170089887A1

公开(公告)日：2017-03-30

申请号：US15374897

申请日：2016-12-09

Applicant: Nano3D Biosciences, Inc

Inventor： Glauco SOUZA ,  Biana GODIN ,  Monica LONGO ,  Jerrie Selga REFUERZO

IPC: G01N33/50 ,  G06T7/62 ,  G06T7/00 ,  G01N33/483 ,  G06K9/00

CPC classification number: G01N33/4833 ,  G06T7/0016 ,  G06T2207/10016 ,  G06T2207/30024

Abstract: Cells are magnetized and then grown in ring shaped 3D culture using a ring magnet. Contractility is measuring by tracking the size of the hole in the 3D cellular ring.

公开(公告)号：US09731391B2

公开(公告)日：2017-08-15

申请号：US14394740

申请日：2013-04-22

Applicant: NANO3D BIOSCIENCES INC.

Inventor： Glauco R. Souza ,  Jianbo Chen

IPC: B23Q1/03 ,  G03B15/00 ,  G02B27/02 ,  G03B17/56 ,  H04N1/00 ,  G01N21/25 ,  G01N21/64

CPC classification number: B23Q1/03 ,  G01N21/253 ,  G01N21/6452 ,  G02B27/028 ,  G03B15/00 ,  G03B17/56 ,  H04N1/00541

Abstract: Camera phone accessories for using the camera phone to collect scientific data.

公开(公告)号：US20160137974A1

公开(公告)日：2016-05-19

申请号：US14965702

申请日：2015-12-10

Applicant: Nano3D Biosciences, Inc

Inventor： Glauco SOUZA

IPC: C12N5/00 ,  C12M1/26 ,  C12M1/32 ,  C12M1/00 ,  C12N13/00 ,  C12M1/12

CPC classification number: C12N5/0062 ,  B01L3/50853 ,  B01L2300/046 ,  B01L2300/0803 ,  B01L2300/0829 ,  B01L2400/043 ,  C12M23/10 ,  C12M23/12 ,  C12M23/38 ,  C12M33/00 ,  C12M33/04 ,  C12M35/06 ,  C12M47/04 ,  C12N13/00

Abstract: Devices for magnetic 3d culture are described including magnetic lids/bases for single Petri plates and adjustable height cap for same. Similar devices for multi-magnet culture plates wherein multiwell plates have all adjacent magnets orientated in the opposite polarity, and methods of making same.

Abstract translation: 描述了用于磁性3d培养的装置，其包括用于单个培养皿的磁性盖/底座和用于其的可调高度帽。 用于多磁板培养板的类似装置，其中多孔板具有以相反极性取向的所有相邻磁体及其制造方法。

公开(公告)号：US20180000936A1

公开(公告)日：2018-01-04

申请号：US15708089

申请日：2017-09-18

Applicant: NANO3D BIOSCIENCES INC

Inventor： Glauco R. SOUZA

IPC: A61K41/00 ,  A61L27/18 ,  G01N33/543 ,  A61L27/38 ,  A61N2/00 ,  C12N5/00 ,  G01N33/58 ,  A61L27/50 ,  B03C1/30 ,  B03C1/01 ,  B03C1/034

CPC classification number: A61K41/0052 ,  A61L27/18 ,  A61L27/38 ,  A61L27/50 ,  A61L2400/12 ,  A61L2430/20 ,  A61L2430/30 ,  A61N2/00 ,  B03C1/01 ,  B03C1/034 ,  B03C1/30 ,  B03C2201/26 ,  C12N5/0062 ,  C12N5/0068 ,  C12N2529/00 ,  G01N33/54346 ,  G01N33/587

Abstract: Methods of making and using a magnetic ECM are disclosed. The ECM comprises positively and negatively charged nanoparticles, wherein one of said nanoparticles contains a magnetically responsive element. When the magnetic ECM is seeded with cells, the cells will be magnetized and can be levitated for 3-D cell culture.

公开(公告)号：US09764030B2

公开(公告)日：2017-09-19

申请号：US14404469

申请日：2013-06-11

Applicant: NANO3D BIOSCIENCES INC.

Inventor： Glauco R. Souza

IPC: C12N5/00 ,  A61K41/00 ,  A61N2/00 ,  A61L27/18 ,  A61L27/38 ,  A61L27/50 ,  G01N33/543 ,  G01N33/58 ,  B03C1/01 ,  B03C1/034 ,  B03C1/30

CPC classification number: A61K41/0052 ,  A61L27/18 ,  A61L27/38 ,  A61L27/50 ,  A61L2400/12 ,  A61L2430/20 ,  A61L2430/30 ,  A61N2/00 ,  B03C1/01 ,  B03C1/034 ,  B03C1/30 ,  B03C2201/26 ,  C12N5/0062 ,  C12N5/0068 ,  C12N2529/00 ,  G01N33/54346 ,  G01N33/587

Abstract: Methods of making and using a magnetic ECM are disclosed. The ECM comprises positively and negatively charged nanoparticles, wherein one of said nanoparticles contains a magnetically responsive element. When the magnetic ECM is seeded with cells, the cells will be magnetized and can be levitated for 3-D cell culture.

公开(公告)号：US20150275165A9

公开(公告)日：2015-10-01

申请号：US13762103

申请日：2013-02-07

Applicant: Nano3D Biosciences, Inc

Inventor： Glauco SOUZA

IPC: C12M1/00 ,  B01L3/02

CPC classification number: C12M47/02 ,  B01L3/021 ,  B01L3/0217 ,  B01L3/0289 ,  B01L2200/0657 ,  B01L2300/0838 ,  B01L2400/043 ,  C12M23/10 ,  C12M23/12 ,  C12M23/38 ,  C12M33/04 ,  C12M35/06

Abstract: Devices for magnetic 3d culture are described including magnetic lids/bases for single Petri plates, adjustable height cap for same, as well similar devices for multi-magnet culture plates. A pen-like device for sterilely lifting and moving cells is also described, and this magnetic pipettor can also exist in multi-well magnetic pipettor formats.

Abstract translation: 描述了用于磁性3d培养的装置，其包括用于单个培养板的磁性盖/底座，用于相同的可调高度盖，以及用于多磁体培养板的类似装置。 还描述了用于无菌提升和移动细胞的笔状装置，并且该磁性移液器也可以以多孔磁吸管器形式存在。

公开(公告)号：US20140220672A1

公开(公告)日：2014-08-07

申请号：US13762103

申请日：2013-02-07

Applicant: Nano3D Biosciences, Inc

Inventor： Glauco SOUZA

IPC: C12M1/00 ,  B01L3/02

CPC classification number: C12M47/02 ,  B01L3/021 ,  B01L3/0217 ,  B01L3/0289 ,  B01L2200/0657 ,  B01L2300/0838 ,  B01L2400/043 ,  C12M23/10 ,  C12M23/12 ,  C12M23/38 ,  C12M33/04 ,  C12M35/06

Abstract: Devices for magnetic 3d culture are described including magnetic lids/bases for single Petri plates, adjustable height cap for same, as well similar devices for multi-magnet culture plates. A pen-like device for sterilely lifting and moving cells is also described, and this magnetic pipettor can also exist in multi-well magnetic pipettor formats.

Abstract translation: 描述了用于磁性3d培养的装置，其包括用于单个培养板的磁性盖/底座，用于相同的可调高度盖，以及用于多磁体培养板的类似装置。 还描述了用于无菌提升和移动细胞的笔状装置，并且该磁性移液器也可以以多孔磁吸管器形式存在。

公开(公告)号：US10265396B2

公开(公告)日：2019-04-23

申请号：US15708089

申请日：2017-09-18

Applicant: NANO3D BIOSCIENCES INC

Inventor： Glauco R. Souza

IPC: A61K41/00 ,  A61N2/00 ,  C12N5/00 ,  A61L27/18 ,  A61L27/38 ,  A61L27/50 ,  G01N33/543 ,  G01N33/58 ,  B03C1/01 ,  B03C1/034 ,  B03C1/30

Abstract: Methods of making and using a magnetic ECM are disclosed. The ECM comprises positively and negatively charged nanoparticles, wherein one of said nanoparticles contains a magnetically responsive element. When the magnetic ECM is seeded with cells, the cells will be magnetized and can be levitated for 3-D cell culture.

公开(公告)号：US09688955B2

公开(公告)日：2017-06-27

申请号：US14536388

申请日：2014-11-07

Applicant: NANO3D BIOSCIENCES INC.

Inventor： Glauco R. Souza

IPC: C12N13/00 ,  C12N5/00 ,  C12N5/07 ,  C12Q1/02 ,  C12Q1/24 ,  B03C1/015 ,  B03C1/30 ,  B82Y25/00 ,  G01N33/543 ,  G01N33/544 ,  G01R33/12 ,  H01F1/00 ,  H01F1/08 ,  H01F1/113 ,  H01F1/44 ,  C40B30/04 ,  G01N33/566 ,  G01N33/68

CPC classification number: C12N5/0062 ,  B03C1/015 ,  B03C1/30 ,  B03C2201/26 ,  B82Y25/00 ,  C12N5/00 ,  C12N2513/00 ,  C12N2529/00 ,  C12N2531/00 ,  C12N2533/10 ,  C12N2533/32 ,  G01N33/54326 ,  G01N33/544 ,  G01R33/1269 ,  H01F1/0045 ,  H01F1/0063 ,  H01F1/083 ,  H01F1/113 ,  H01F1/445 ,  Y10S977/773 ,  Y10S977/89 ,  Y10S977/904 ,  Y10S977/923

Abstract: A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field.

公开(公告)号：US20150110752A1

公开(公告)日：2015-04-23

申请号：US14404469

申请日：2013-06-11

Applicant: NANO3D BIOSCIENCES INC.

Inventor： Glauco R. Souza

IPC: A61K41/00 ,  C12N5/00

CPC classification number: A61K41/0052 ,  A61L27/18 ,  A61L27/38 ,  A61L27/50 ,  A61L2400/12 ,  A61L2430/20 ,  A61L2430/30 ,  A61N2/00 ,  B03C1/01 ,  B03C1/034 ,  B03C1/30 ,  B03C2201/26 ,  C12N5/0062 ,  C12N5/0068 ,  C12N2529/00 ,  G01N33/54346 ,  G01N33/587

Abstract: Methods of making and using a magnetic ECM are disclosed. The ECM comprises positively and negatively charged nanoparticles, wherein one of said nanoparticles contains a magnetically responsive element. When the magnetic ECM is seeded with cells, the cells will be magnetized and can be levitated for 3-D cell culture.

Abstract translation: 公开了制造和使用磁性ECM的方法。 ECM包含带正电荷和带负电荷的纳米颗粒，其中所述纳米颗粒之一含有磁响应元件。 当磁性ECM接种细胞时，细胞将被磁化并且可以悬浮以进行3-D细胞培养。