公开(公告)号：US20250123291A1

公开(公告)日：2025-04-17

申请号：US18887496

申请日：2024-09-17

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Yan Chen ,  Aled Jones ,  Oscar Potter

IPC: G01N33/68 ,  G01N33/58

Abstract: Methods of quantifying IgG antibodies containing high mannose N-glycans. Methods include immobilizing a protein capable of binding to IgG antibodies containing high mannose N-glycans to a substrate. Methods include adding a sample comprising IgG antibodies containing high mannose N-glycans to the immobilized protein to form a binding mixture. Methods further include adding a high mannose binder protein (HMB)-dye conjugate to the binding mixture such that the HMB-dye conjugate binds to high mannose N-glycans present in the sample. Methods include detecting the IgG antibodies containing high mannose N-glycans bound to the HMB-dye conjugate. Kits for the quantifying IgG antibodies containing high mannose N-glycans are also disclosed.

公开(公告)号：US20250110118A1

公开(公告)日：2025-04-03

申请号：US18650999

申请日：2024-04-30

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Eric LYSTER ,  Oscar Potter ,  Randall Robinson

IPC: G01N33/53 ,  G01N30/72 ,  G01N30/86 ,  G16B40/10

Abstract: In some examples, an apparatus may include a retention time (RT) receiver that is executed by at least one hardware processor, to receive glucose ladder retention times (RTs), and receive neutral and charged glycan RTs. An RT analyzer that is executed by the at least one hardware processor may determine, based on application of the glucose ladder RTs, and the neutral and charged glycan RTs to expected glucose unit (GU) values, adjusted GU values. Further, an expected RT generator that is executed by the at least one hardware processor may determine, based on the adjusted GU values, expected RTs for specified glycans.