公开(公告)号：US20250123291A1

公开(公告)日：2025-04-17

申请号：US18887496

申请日：2024-09-17

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Yan Chen ,  Aled Jones ,  Oscar Potter

IPC: G01N33/68 ,  G01N33/58

Abstract: Methods of quantifying IgG antibodies containing high mannose N-glycans. Methods include immobilizing a protein capable of binding to IgG antibodies containing high mannose N-glycans to a substrate. Methods include adding a sample comprising IgG antibodies containing high mannose N-glycans to the immobilized protein to form a binding mixture. Methods further include adding a high mannose binder protein (HMB)-dye conjugate to the binding mixture such that the HMB-dye conjugate binds to high mannose N-glycans present in the sample. Methods include detecting the IgG antibodies containing high mannose N-glycans bound to the HMB-dye conjugate. Kits for the quantifying IgG antibodies containing high mannose N-glycans are also disclosed.