公开(公告)号：US20230025343A1

公开(公告)日：2023-01-26

申请号：US17778712

申请日：2020-11-20

Applicant: API CORPORATION

Inventor： Takanobu IURA ,  Yasumasa DEKISHIMA ,  Takeshi SAKAMOTO ,  Mari HARA ,  Hirotoshi HIRAOKA ,  Harald GRÖGER ,  Jieun CHOI

IPC: C12P17/16 ,  C12N9/04

Abstract: The present invention provides a carbonyl reductase having the activity of reducing a carbonyl group-containing compound to convert the compound into an optically active compound, and a production method of an optically active compound using the enzyme. Specifically, a carbonyl reductase having one or more mutations in which the 54th aspartic acid, the 157th methionine, the 170th alanine, the 211th isoleucine, the 214th methionine, and the 249th methionine are each substituted by other specific amino acid in the amino acid sequence shown in SEQ ID NO: 1 or a homologue of the amino acid sequence, and a production method of an optically active compound using the same are provided.