公开(公告)号：US20230323317A1

公开(公告)日：2023-10-12

申请号：US18091687

申请日：2022-12-30

Applicant: API CORPORATION

Inventor： Toyokazu YOSHIDA ,  Koichi ISHIDA ,  Ryoma MIYAKE ,  Takanobu IURA ,  Hiroshi KAWABATA

IPC: C12N9/18 ,  C12P7/62 ,  C12P41/00

CPC classification number: C12N9/18 ,  C12P7/62 ,  C12P41/00 ,  C12Y301/01

Abstract: The present invention provides a novel hydrolase that can industrially produce optically highly pure (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropane carboxylic acid with high efficiency at low costs, and a production method using the hydrolase.

公开(公告)号：US20200283390A1

公开(公告)日：2020-09-10

申请号：US16851402

申请日：2020-04-17

Applicant: API CORPORATION

Inventor： Naoyuki WATANABE ,  Takanobu IURA ,  Hideki OOMIYA ,  Masaki NAGAHAMA

IPC: C07D215/14 ,  C12P17/12

Abstract: Production of pitavastatin calcium safely on an industrial scale with a high yield and high selectivity at low cost. A method of producing pitavastatin calcium including step (i) for acetalizing a compound represented by the formula (1) to give a compound represented by the formula (3), step (ii) for reacting a compound represented by the formula (3) with an acid to give a compound represented by the formula (4), and step (iii) for hydrolyzing a compound represented by the formula (4) and reacting same with a calcium compound.

公开(公告)号：US20210024905A1

公开(公告)日：2021-01-28

申请号：US17043339

申请日：2019-03-29

Applicant: API CORPORATION

Inventor： Toyokazu YOSHIDA ,  Koichi ISHIDA ,  Ryoma MIYAKE ,  Takanobu IURA ,  Hiroshi KAWABATA

IPC: C12N9/18 ,  C12P41/00 ,  C12P7/62

Abstract: The present invention provides a novel hydrolase that can industrially produce optically highly pure (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropane carboxylic acid with high efficiency at low costs, and a production method using the hydrolase.

公开(公告)号：US20180222865A1

公开(公告)日：2018-08-09

申请号：US15749377

申请日：2016-08-05

Applicant: API CORPORATION

Inventor： Naoyuki WATANABE ,  Takanobu IURA ,  Hideki OOMIYA ,  Masaki NAGAHAMA

IPC: C07D215/14 ,  C12P17/12

Abstract: Production of pitavastatin calcium safely on an industrial scale with a high yield and high selectivity at low cost. A method of producing pitavastatin calcium including step (i) for acetalizing a compound represented by the formula (1) to give a compound represented by the formula (3), step (ii) for reacting a compound represented by the formula (3) with an acid to give a compound represented by the formula (4), and step (iii) for hydrolyzing a compound represented by the formula (4) and reacting same with a calcium compound.

公开(公告)号：US20230025343A1

公开(公告)日：2023-01-26

申请号：US17778712

申请日：2020-11-20

Applicant: API CORPORATION

Inventor： Takanobu IURA ,  Yasumasa DEKISHIMA ,  Takeshi SAKAMOTO ,  Mari HARA ,  Hirotoshi HIRAOKA ,  Harald GRÖGER ,  Jieun CHOI

IPC: C12P17/16 ,  C12N9/04

Abstract: The present invention provides a carbonyl reductase having the activity of reducing a carbonyl group-containing compound to convert the compound into an optically active compound, and a production method of an optically active compound using the enzyme. Specifically, a carbonyl reductase having one or more mutations in which the 54th aspartic acid, the 157th methionine, the 170th alanine, the 211th isoleucine, the 214th methionine, and the 249th methionine are each substituted by other specific amino acid in the amino acid sequence shown in SEQ ID NO: 1 or a homologue of the amino acid sequence, and a production method of an optically active compound using the same are provided.

公开(公告)号：US20220098623A1

公开(公告)日：2022-03-31

申请号：US17284912

申请日：2020-04-17

Applicant: API CORPORATION

Inventor： Haruka SASANO ,  Takanobu IURA ,  Kenji OKI

IPC: C12P7/02 ,  C12P7/26

Abstract: A method for producing a compound represented by formula (3) including bringing a carbon-carbon double bond reductase, a microorganism or cell having an ability to produce the enzyme, a processed product of the microorganism or cell, and/or a culture solution containing the enzyme which is obtained by culturing the microorganism or cell, and a carbonyl reductase, a microorganism or cell having an ability to produce the enzyme, a processed product of the microorganism or cell, and/or a culture solution containing the enzyme which is obtained by culturing the microorganism or cell into contact with a compound represented by formula (1) to obtain a compound represented by formula (3):