公开(公告)号：US20170028408A1

公开(公告)日：2017-02-02

申请号：US15302408

申请日：2015-04-02

Applicant: APOCELL, INC.

Inventor： Anoop MENACHERY ,  Ronald PETHIG

IPC: B03C5/00 ,  G01N33/487 ,  G01N27/447

CPC classification number: B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N27/44713 ,  G01N27/44721 ,  G01N33/48707

Abstract: The present invention provides a new method for accurately identifying DEP cross-over frequencies of one or more particles in a sample, and quickly and efficiently conveying that information to assist in the separation, e.g., DEP separation, or analysis of the one of more particles under examination or investigation. The present invention also provides an apparatus and method for monitoring the dielectrophoretic response of one or more particles and determining the DEP cross-over frequency of particles of interest.

Abstract translation: 本发明提供了一种用于准确地识别样品中一种或多种颗粒的DEP交叉频率的新方法，并且快速有效地传达该信息以帮助分离，例如DEP分离或分析更多的颗粒之一 正在考察或调查。 本发明还提供了一种用于监测一种或多种颗粒的介电电泳响应并确定感兴趣的颗粒的DEP交叉频率的装置和方法。

公开(公告)号：US20160209299A1

公开(公告)日：2016-07-21

申请号：US14914714

申请日：2014-08-28

Applicant: APOCELL, INC.

Inventor： Anoop MENACHERY ,  Vishal GUPTA ,  David K HASEGAWA ,  Ronald PETHIG

IPC: G01N1/10 ,  G01N33/483 ,  G01N27/447

CPC classification number: G01N1/10 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N27/44717 ,  G01N27/44743 ,  G01N27/44769 ,  G01N30/0005 ,  G01N33/4833 ,  G01N2030/0065

Abstract: Methods and apparatuses for target particle separation and isolation are disclosed. The apparatuses are flow chambers that include an infusion port configured to introduce a sample, and an elution flow port configured to receive an elution buffer. The flow chamber also includes an electrode coupled to a floor of the flow chamber, wherein the electrode includes at least a first zone with a first inter-electrode pitch and a second zone with a second inter-electrode pitch. The first zone generates a first electric field and the second zone generates a second electric field of a different magnitude than the first electric field when an electric signal is applied. The first electric field and the second electric field separate the target particle from the fluidic suspension via dielectrophoresis. The flow chamber also includes a waste disposal port that discharges the waste material, and a collection port configured that collects a target particle enriched sample.

Abstract translation: 公开了用于目标颗粒分离和分离的方法和装置。 所述装置是流动室，其包括被配置为引入样品的输注端口和被配置为接收洗脱缓冲液的洗脱流动端口。 流动室还包括耦合到流动室的底板的电极，其中电极至少包括具有第一电极间距的第一区和具有第二电极间距的第二区。 第一区产生第一电场，并且当施加电信号时，第二区产生与第一电场不同大小的第二电场。 第一电场和第二电场通过介电电泳将目标颗粒与流体悬浮液分离。 流动室还包括排出废料的废物处理口，以及收集端口，其被配置为收集富含目标颗粒的样品。