公开(公告)号：US20160209299A1

公开(公告)日：2016-07-21

申请号：US14914714

申请日：2014-08-28

Applicant: APOCELL, INC.

Inventor： Anoop MENACHERY ,  Vishal GUPTA ,  David K HASEGAWA ,  Ronald PETHIG

IPC: G01N1/10 ,  G01N33/483 ,  G01N27/447

CPC classification number: G01N1/10 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N27/44717 ,  G01N27/44743 ,  G01N27/44769 ,  G01N30/0005 ,  G01N33/4833 ,  G01N2030/0065

Abstract: Methods and apparatuses for target particle separation and isolation are disclosed. The apparatuses are flow chambers that include an infusion port configured to introduce a sample, and an elution flow port configured to receive an elution buffer. The flow chamber also includes an electrode coupled to a floor of the flow chamber, wherein the electrode includes at least a first zone with a first inter-electrode pitch and a second zone with a second inter-electrode pitch. The first zone generates a first electric field and the second zone generates a second electric field of a different magnitude than the first electric field when an electric signal is applied. The first electric field and the second electric field separate the target particle from the fluidic suspension via dielectrophoresis. The flow chamber also includes a waste disposal port that discharges the waste material, and a collection port configured that collects a target particle enriched sample.

Abstract translation: 公开了用于目标颗粒分离和分离的方法和装置。 所述装置是流动室，其包括被配置为引入样品的输注端口和被配置为接收洗脱缓冲液的洗脱流动端口。 流动室还包括耦合到流动室的底板的电极，其中电极至少包括具有第一电极间距的第一区和具有第二电极间距的第二区。 第一区产生第一电场，并且当施加电信号时，第二区产生与第一电场不同大小的第二电场。 第一电场和第二电场通过介电电泳将目标颗粒与流体悬浮液分离。 流动室还包括排出废料的废物处理口，以及收集端口，其被配置为收集富含目标颗粒的样品。