公开(公告)号：US20200209250A1

公开(公告)日：2020-07-02

申请号：US16729067

申请日：2019-12-27

Applicant: Abbott Laboratories

Inventor： Qiaoqiao Ruan ,  Patrick J. Macdonald ,  Kerry M. Swift ,  Sergey Y. Tetin ,  Brenda Calfin ,  Zhen Lin ,  Richard Haack ,  Mark R. Pope ,  John Prostko ,  Xiaoxing Qiu ,  Felicia Bogdan

IPC: G01N33/58 ,  G01N15/14 ,  G01N33/543

Abstract: The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

公开(公告)号：US20240377405A1

公开(公告)日：2024-11-14

申请号：US18761492

申请日：2024-07-02

Applicant: Abbott Laboratories

Inventor： Qiaoqiao Ruan ,  Patrick J. Macdonald ,  Kerry M. Swift ,  Sergey Y. Tetin ,  Brenda Calfin ,  Zhen Lin ,  Richard Haack ,  Mark R. Pope ,  John Prostko ,  Xiaoxing Qiu ,  M. Felicia Bogdan

IPC: G01N33/58 ,  G01N15/10 ,  G01N15/14 ,  G01N33/543

Abstract: The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

公开(公告)号：US12061200B2

公开(公告)日：2024-08-13

申请号：US16729067

申请日：2019-12-27

Applicant: Abbott Laboratories

Inventor： Qiaoqiao Ruan ,  Patrick J. Macdonald ,  Kerry M. Swift ,  Sergey Y. Tetin ,  Brenda Calfin ,  Zhen Lin ,  Richard Haack ,  Mark R. Pope ,  John Prostko ,  Xiaoxing Qiu ,  M. Felicia Bogdan

IPC: G01N33/53 ,  G01N15/14 ,  G01N33/543 ,  G01N33/58 ,  G01N15/10

CPC classification number: G01N33/582 ,  G01N15/14 ,  G01N33/54346 ,  G01N33/587 ,  G01N2015/1018

Abstract: The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

公开(公告)号：US20220291223A1

公开(公告)日：2022-09-15

申请号：US17633071

申请日：2020-08-06

Applicant: ABBOTT LABORATORIES

Inventor： Stefan J. Hershberger ,  Richard A. Haack ,  Qiaoqiao Ruan ,  Quinn Best ,  Brian M. Bax ,  Kerry M. Swift ,  Sergey Y. Tetin

IPC: G01N33/58 ,  G01N33/543 ,  C09B15/00 ,  C09K11/07 ,  C09B11/08

Abstract: Disclosed herein are compounds, conjugates, and methods that may be used to detect the presence of an analyte in a sample, such as a biological sample.

公开(公告)号：US20220276256A1

公开(公告)日：2022-09-01

申请号：US17633065

申请日：2020-08-06

Applicant: ABBOTT LABORATORIES

Inventor： Mark R. Pope ,  Stefan J. Hershberger ,  Qiaoqiao Ruan ,  Kerry M. Swift ,  Sergey Y. Tetin ,  Patrick Macdonald ,  Richard A. Haack

IPC: G01N33/58 ,  G01N33/543

Abstract: The disclosure provides kits and methods for detecting a substance that interferes with detection of an analyte in a sample and for expanding the dynamic range and reducing the hook effect of an immunoassay. The kits and methods employ two conjugates with two different detectable labels, at least one of which is a chemiluminescent compound of Formula (I).