公开(公告)号：US11817185B2

公开(公告)日：2023-11-14

申请号：US16846185

申请日：2020-04-10

Applicant: Agilent Technologies, Inc.

Inventor： Stephen P. Madden ,  Steven M. Fischer

IPC: G16C20/30 ,  G16C20/70 ,  G16C20/50 ,  G16H10/40

CPC classification number: G16C20/30 ,  G16C20/50 ,  G16C20/70 ,  G16H10/40

Abstract: A method for analyzing samples utilizing stable label isotope tracing includes receiving mass spectrometry (MS) data generated by an MS system performing untargeted data acquisition on a plurality of samples, performing untargeted feature extraction on the unlabeled compound data to generate a data set of first extracted features, generating a plurality of empirical molecular formulas respectively corresponding to the first extracted features, performing targeted isotopologue extraction on the labeled compound data to generate a data set of second extracted features, wherein the targeted isotopologue extraction is based on the empirical molecular formula and retention time of each first extracted feature, and identifying one or more groups of isotopologues from the second extracted features.

公开(公告)号：US20170330740A1

公开(公告)日：2017-11-16

申请号：US15461337

申请日：2017-03-16

Applicant: Agilent Technologies, Inc. ,  Memorial Sloan Kettering Cancer Center

Inventor： Steven M. Fischer ,  Justin R. Cross

IPC: H01J49/16 ,  H01J49/04 ,  H01J49/00

CPC classification number: H01J49/165 ,  H01J49/0027 ,  H01J49/0431

Abstract: Provided herein, among other things, is a method of ionizing a first stream of liquid by an electrospray ion source having a nebulizer, wherein the first stream of liquid may comprise an analyte. In some embodiments, the method may comprise: a) providing the first stream of liquid to the nebulizer; b) adding a second stream of liquid to the first stream of liquid, wherein the second stream of liquid comprises a co-solvent that has a relatively high boiling point and an enhancement solvent that a relatively high boiling; and c) nebulizing and ionizing the resulting liquid.

公开(公告)号：US11808675B2

公开(公告)日：2023-11-07

申请号：US16874499

申请日：2020-05-14

Applicant: Agilent Technologies, Inc.

Inventor： Genevieve Van de Bittner ,  James Alexander Apffel ,  Steven M. Fischer ,  Christine A. Miller ,  Kristin Briana Bernick

IPC: G01N1/28 ,  C12N1/06 ,  B01D15/00 ,  C12N15/10 ,  B01D11/02 ,  C07K1/14

CPC classification number: G01N1/28 ,  B01D11/0288 ,  B01D15/00 ,  C07K1/145 ,  C12N1/06 ,  C12N15/1006

Abstract: Reagents and methods for extracting and stabilizing metabolites at room temperature and methods in which metabolites and one or more of proteins, lipids and nucleic acids are extracted from a single sample in a unified workflow.

公开(公告)号：US10373816B2

公开(公告)日：2019-08-06

申请号：US15461337

申请日：2017-03-16

Applicant: Agilent Technologies, Inc. ,  Memorial Sloan Kettering Cancer Center

Inventor： Steven M. Fischer ,  Justin R. Cross

IPC: H01J49/00 ,  H01J49/04 ,  H01J49/16

Abstract: Provided herein, among other things, is a method of ionizing a first stream of liquid by an electrospray ion source having a nebulizer, wherein the first stream of liquid may comprise an analyte. In some embodiments, the method may comprise: a) providing the first stream of liquid to the nebulizer; b) adding a second stream of liquid to the first stream of liquid, wherein the second stream of liquid comprises a co-solvent that has a relatively high boiling point and an enhancement solvent that a relatively high boiling; and c) nebulizing and ionizing the resulting liquid.