公开(公告)号：US20210139871A1

公开(公告)日：2021-05-13

申请号：US16954276

申请日：2018-12-10

Applicant: BASF SE

Inventor： Xuqiu TAN ,  Kenneth BARRETT ,  Jared DENNIS ,  Dongmei REN ,  Corey DODGE ,  Bin ZHANG

IPC: C12N9/22 ,  C11D3/386 ,  A23L33/18 ,  A23K20/147 ,  A23L29/00 ,  A23K20/189

Abstract: Disclosed herein are polypeptides having nuclease activity. Some of the polypeptides having nuclease activity were generated by an improved gene site mutagenesis (“GSSM”) method or the tailored multi-site combinatorial assembly (“TMCA”) method. Also disclosed are compositions and kits comprising the polypeptides having nuclease activity, and methods for making and using these polypeptides, compositions and kits.