公开(公告)号：US20200318089A1

公开(公告)日：2020-10-08

申请号：US16757993

申请日：2018-10-17

Applicant: BASF SE

Inventor： Hugo URBINA ,  Tong LI ,  Jared DENNIS ,  Xuqiu TAN ,  Adrienne HUSTON DAVENPORT ,  Jochen KUTSCHER ,  Kristine W. CHANG

IPC: C12N9/26

Abstract: Variant polypeptides having beta-amylase activity and methods of making and using the enzymes in baking, detergents, personal care products, in the processing of textiles, in pulp and paper processing, in the production of ethanol, lignocellulosic ethanol, or syrups; as viscosity breakers in oilfield and mining industries.

公开(公告)号：US20220251540A1

公开(公告)日：2022-08-11

申请号：US16954092

申请日：2018-12-18

Applicant: BASF SE

Inventor： Jared DENNIS ,  Xuqiu TAN ,  Robin CAI

IPC: C12N15/10 ,  C12N1/20 ,  C12N15/70

Abstract: Disclosed herein is an improved Gene Site Saturation Mutagenesis (GSSM) method for producing a plurality of modified polynucleotides and/or polypeptides, creating specific changes to a gene, and reassembling mutations or changes at one or more sites.

公开(公告)号：US20210139871A1

公开(公告)日：2021-05-13

申请号：US16954276

申请日：2018-12-10

Applicant: BASF SE

Inventor： Xuqiu TAN ,  Kenneth BARRETT ,  Jared DENNIS ,  Dongmei REN ,  Corey DODGE ,  Bin ZHANG

IPC: C12N9/22 ,  C11D3/386 ,  A23L33/18 ,  A23K20/147 ,  A23L29/00 ,  A23K20/189

Abstract: Disclosed herein are polypeptides having nuclease activity. Some of the polypeptides having nuclease activity were generated by an improved gene site mutagenesis (“GSSM”) method or the tailored multi-site combinatorial assembly (“TMCA”) method. Also disclosed are compositions and kits comprising the polypeptides having nuclease activity, and methods for making and using these polypeptides, compositions and kits.