公开(公告)号：US20200318089A1

公开(公告)日：2020-10-08

申请号：US16757993

申请日：2018-10-17

Applicant: BASF SE

Inventor： Hugo URBINA ,  Tong LI ,  Jared DENNIS ,  Xuqiu TAN ,  Adrienne HUSTON DAVENPORT ,  Jochen KUTSCHER ,  Kristine W. CHANG

IPC: C12N9/26

Abstract: Variant polypeptides having beta-amylase activity and methods of making and using the enzymes in baking, detergents, personal care products, in the processing of textiles, in pulp and paper processing, in the production of ethanol, lignocellulosic ethanol, or syrups; as viscosity breakers in oilfield and mining industries.

公开(公告)号：US20210254082A1

公开(公告)日：2021-08-19

申请号：US15734682

申请日：2019-06-03

Applicant: BASF SE

Inventor： Xuqiu TAN ,  Ruorong CAI ,  Jingping ZHONG ,  Melissa Ann SCRANTON ,  Michael SPEER

IPC: C12N15/81 ,  C07K14/39

Abstract: A promoter operably linked to a gene encoding a protein is disclosed. The promoter drives expression of the protein in a yeast cell in the absence of methanol. Also disclosed are vectors, host cells and expression systems that include the promoter, as well as methods of using the promoter to express proteins in yeast.

公开(公告)号：US20220220463A1

公开(公告)日：2022-07-14

申请号：US17605919

申请日：2020-04-21

Applicant: BASF SE

Inventor： Hugo URBINA ,  Kristine W. CHANG ,  Tuan TRAN ,  Gina Ning Lee LOWE ,  Adrienne HUSTON DAVENPORT ,  Xuqiu TAN ,  Tong LI ,  Stefan JENEWEIN ,  Stefan SEEMAYER ,  Jochen KUTSCHER

IPC: C12N9/26 ,  A21D8/04

Abstract: The present invention relates to variants of a beta-amylase which have an increased % residual exoamylase activity compared to the parent beta-amylase after heat treatment. The present invention also relates to methods of making the variant beta-amylase and the use of the variant beta-amylase in baking, detergents, personal care products, in the processing of textiles, in pulp and paper processing, in the production of ethanol, lignocellulosic ethanol or syrups and as viscosity breaker in oilfield and mining industries.

公开(公告)号：US20210198643A1

公开(公告)日：2021-07-01

申请号：US17057853

申请日：2019-05-29

Applicant: BASF SE

Inventor： Hugo URBINA ,  Asfia QURESHI ,  Adrienne HUSTON DAVENPORT ,  Xuqiu TAN ,  Jochen KUTSCHER ,  Andreas FUNKE ,  Michael SEITTER ,  Stefan HAEFNER ,  Anh-Huy Phan LE

IPC: C12N9/28 ,  A21D8/04

Abstract: The present invention relates to variants of an alpha-amylase which have an increased exoamylase activity compared to the parent alpha-amylase. The present invention also relates to methods of making the variant alpha-amylase and the use of the variant alpha-amylase in baking, detergents, personal care products, in the processing of textiles, in pulp and paper processing, in the production of ethanol, lignocellulosic ethanol or syrups and as viscosity breaker in oilfield and mining industries.

公开(公告)号：US20190053501A1

公开(公告)日：2019-02-21

申请号：US16079255

申请日：2017-02-15

Applicant: BASF SE

Inventor： Cristina POP ,  Adrienne HUSTON DAVENPORT ,  Michael LISZKA ,  Xuqiu TAN ,  Andreas FUNKE ,  Jochen KUTSCHER ,  Michael SEITTER ,  Stefan HAEFNER

IPC: A21D8/04 ,  C12N9/16 ,  C12N9/26 ,  C12N9/10 ,  C12N9/04

Abstract: Lipase enzymes and methods of using the lipases in a baking for improving the volume, stability, tolerance of a baked product and/or reducing and reducing or eliminating the use of DATEM.

公开(公告)号：US20220251540A1

公开(公告)日：2022-08-11

申请号：US16954092

申请日：2018-12-18

Applicant: BASF SE

Inventor： Jared DENNIS ,  Xuqiu TAN ,  Robin CAI

IPC: C12N15/10 ,  C12N1/20 ,  C12N15/70

Abstract: Disclosed herein is an improved Gene Site Saturation Mutagenesis (GSSM) method for producing a plurality of modified polynucleotides and/or polypeptides, creating specific changes to a gene, and reassembling mutations or changes at one or more sites.

公开(公告)号：US20210230708A1

公开(公告)日：2021-07-29

申请号：US15734768

申请日：2019-06-03

Applicant: BASF SE

Inventor： Xuqiu TAN ,  Corey DODGE ,  Kevin Li WONG ,  Aileen Aboy MILAN ,  Samantha ORCHARD ,  Ruorong CAI

IPC: C12Q1/6895 ,  C12N15/81 ,  C12P21/00

Abstract: Disclosed herein are methods of identifying promoters that drive protein expression independently of methanol and are useful in driving protein expression in yeast. The method may comprise the steps of: fermenting yeast cells under at least one fermentation condition in the absence of methanol, collecting samples at different times during fermentation under the at least one fermentation conditions, determining the relative mRNA levels associated with native yeast genes in the samples, identifying one or more of the native yeast genes associated with higher than average levels of mRNA and determining putative promoters associated with the higher than average levels of mRNA encoding the native yeast genes, making expression constructs, each construct comprising one of the identified putative promoters and a gene encoding a marker protein and introducing the expression constructs into yeast cells, culturing the yeast cells comprising the expression constructs in the absence of methanol, determining marker protein expression by the cultured yeast cells and comparing marker protein expression driven by each of the putative promoters to identify promoters that drive protein expression independently of methanol and are useful in driving protein expression in yeast.

公开(公告)号：US20210139871A1

公开(公告)日：2021-05-13

申请号：US16954276

申请日：2018-12-10

Applicant: BASF SE

Inventor： Xuqiu TAN ,  Kenneth BARRETT ,  Jared DENNIS ,  Dongmei REN ,  Corey DODGE ,  Bin ZHANG

IPC: C12N9/22 ,  C11D3/386 ,  A23L33/18 ,  A23K20/147 ,  A23L29/00 ,  A23K20/189

Abstract: Disclosed herein are polypeptides having nuclease activity. Some of the polypeptides having nuclease activity were generated by an improved gene site mutagenesis (“GSSM”) method or the tailored multi-site combinatorial assembly (“TMCA”) method. Also disclosed are compositions and kits comprising the polypeptides having nuclease activity, and methods for making and using these polypeptides, compositions and kits.