公开(公告)号：US6020126A

公开(公告)日：2000-02-01

申请号：US821524

申请日：1997-03-21

Applicant: Christina Carlsson ,  Maria T. Dulay ,  Mats Jonsson ,  Peter Nielson ,  Bengt Norden ,  Lap-Chee Tsui ,  Richard Zare ,  Julian Zielinski

Inventor： Christina Carlsson ,  Maria T. Dulay ,  Mats Jonsson ,  Peter Nielson ,  Bengt Norden ,  Lap-Chee Tsui ,  Richard Zare ,  Julian Zielinski

IPC: C07H21/00 ,  C12Q1/68 ,  C07H19/00

CPC classification number: C07H21/00 ,  C12Q1/6813

Abstract: A rapid and sensitive detection method capable of reliably distinguishing fragments identical to a defined sequence from fragments containing a single base pair mismatch relative to the defined sequence. The method employs a peptide analog antisense probe, such as a peptide nucleic acid (PNA) probe, which hybridizes with DNA to forms complexes having higher thermal stability, but greater sensitivity to base pair mismatches, than corresponding DNA/DNA complexes. The method may be used in a number of applications, including genetic screening and hybridization sequencing applications.

Abstract translation: 一种快速且灵敏的检测方法，其能够将与所定义的序列相同的片段与相对于所定义的序列的单个碱基对失配的片段可靠地区分开。 该方法使用肽类似物反义探针，例如肽核酸（PNA）探针，其与DNA杂交以形成具有比相应的DNA / DNA复合物更高的热稳定性但对碱基对错配的较高敏感性的复合物。 该方法可用于许多应用，包括遗传筛选和杂交测序应用。

公开(公告)号：US20120081707A1

公开(公告)日：2012-04-05

申请号：US13175017

申请日：2011-07-01

Applicant: Bengt Norden

Inventor： Bengt Norden

IPC: G01J4/00 ,  G01N21/01

CPC classification number: G01N21/19 ,  G01N11/14 ,  G01N21/05 ,  G01N2021/0346

Abstract: The present invention relates to shear flow device 100 comprising cell 110, wherein the cell is static and comprises stationary plates 110a and 110b, and movable slide 130. The device of the present invention can be used in methods of orienting molecules for LD measurement and in methods for creating an oriented macromolecule or macromolecule complex.

Abstract translation: 本发明涉及包括电池110的剪切流动装置100，其中电池是静态的，并且包括固定板110a和110b以及可移动滑动件130.本发明的装置可用于定向分子用于LD测量的方法和 用于产生定向高分子或大分子复合物的方法。