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公开(公告)号：US20080095856A1
公开(公告)日：2008-04-24
申请号：US11748408
申请日：2007-05-14
Applicant: Gunilla Jacobson , Richard Zare , Karin Markides , Rajesh Shinde , Christopher Contag
Inventor： Gunilla Jacobson , Richard Zare , Karin Markides , Rajesh Shinde , Christopher Contag
CPC classification number: A61K9/5153 , A61K9/5031 , A61K9/5036 , A61K9/5042 , A61K9/5192 , A61K47/6935 , A61K47/6937 , A61K49/0093 , B82Y5/00
Abstract: Compositions and methods are provided for preparing nanosized biologically active agents, including agents formulated for target specific drug delivery. The nanosized agents are prepared with supercritical carbon dioxide as an antisolvent, providing nanoparticles whose size, shape, and surroundings are well-controlled. The nanoparticles are made of small molecules, e.g. drugs, anti-oxidants, luciferin, polypeptides, e.g. oligopeptides; polynucleotides, e.g. siRNA, antisense oligonucleotides, etc. In some embodiments, the nanoparticles comprise a polymer coating, which can provide for controlled delivery, targeting, controlled release, and the like. In other embodiments, the nanoparticles comprise a target specific tag for targeting the nanoparticles to a site of interest, e.g. tissue, cell, etc.
Abstract translation: 提供组合物和方法用于制备纳米尺寸生物活性剂，包括配制用于靶特异性药物递送的试剂。 用超临界二氧化碳作为反溶剂制备纳米尺寸剂，提供其尺寸，形状和环境受到良好控制的纳米颗粒。 纳米颗粒由小分子制成。 药物，抗氧化剂，荧光素，多肽等。 寡肽; 多核苷酸，例如 siRNA，反义寡核苷酸等。在一些实施方案中，纳米颗粒包含可提供受控递送，靶向，控制释放等的聚合物涂层。 在其它实施方案中，纳米颗粒包含靶标特异性标签，用于将纳米颗粒靶向感兴趣的位点。 组织，细胞等
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公开(公告)号：US20070176270A1
公开(公告)日：2007-08-02
申请号：US11674072
申请日：2007-02-12
Applicant: Ignacio Zuleta , Richard Zare
Inventor： Ignacio Zuleta , Richard Zare
IPC: H01L23/495
CPC classification number: H01J49/061 , H01J49/0018 , H01L2924/0002 , H01L2924/00
Abstract: A beam modulation device gate is constructed from a silicon material, such as a silicon layer on an silicon on insulator wafer. The device further comprises a set of electrical contacts on the layer. The layer defines a set of electrically conducting silicon material fingers forming an array, wherein each of at least some of the fingers is connected electrically to one of the electrical contacts. The gate may be used in a mass or ion mobility spectrometer. Where the gate is constructed from a silicon on insulator wafer, an insulator layer supports the silicon layer and a handle layer supports the insulator layer. When predetermined electrical potentials are applied to the electrical contacts, at least some of the fingers will be substantially at said predetermined electrical potentials to modulate a beam of charged particles that passes through said array of fingers. A plurality of devices of the type above may be used, where each of the devices modulates the beam so that the beam is deflected along a direction different from direction along which the beam is deflected by any of the remaining devices. A plurality of devices of the type above may be used for a mass gate or charged particle buncher device. For making an ion optical device, an array of fingers is formed in a silicon layer of the silicon on insulator wafer. A portion of a handle layer of the wafer on a side of an insulator layer of the wafer opposite to that of the fingers is removed; and a portion of the insulator layer is removed so that the fingers are connected to the wafer only through the silicon layer and at one end of the fingers.
Abstract translation: 光束调制器件栅极由诸如硅绝缘体晶片上的硅层的硅材料构成。 该装置还包括该层上的一组电触点。 该层限定了一组形成阵列的导电硅材料指状物，其中至少一些指状物中的每一个与电触点之一电连接。 栅极可用于质谱或离子迁移谱仪。 在栅极由硅绝缘体晶片构成的情况下，绝缘体层支撑硅层，并且手柄层支撑绝缘体层。 当预定的电势被施加到电触点时，至少一些指状物将基本处于所述预定的电势，以调制穿过所述指状物阵列的带电粒子束。 可以使用上述类型的多个装置，其中每个装置调制光束，使得光束沿着不同于任何剩余装置偏转光束的方向的方向偏转。 上述类型的多个装置可以用于质量门或带电粒子聚束器装置。 为了制造离子光学器件，在硅绝缘体晶片的硅层中形成指状物阵列。 除去晶片的与手指相反的晶片的绝缘体层一侧的手柄层的一部分; 并且去除绝缘体层的一部分，使得指状物仅通过硅层和手指的一端连接到晶片。
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3.发明申请Immobilized-enzyme microreactor devices for characterization of biomolecular analytes and associated methods 审中-公开用于表征生物分子分析物和相关方法的固定酶微反应器装置公开(公告)号：US20070141550A1
公开(公告)日：2007-06-21
申请号：US10582646
申请日：2004-12-13
Applicant: Richard Zare , Maria Dulay , Quentin Baca , Gabriele Trapp
Inventor： Richard Zare , Maria Dulay , Quentin Baca , Gabriele Trapp
CPC classification number: G01N33/573 , C12N11/08 , C12N11/14 , C12Q1/37 , G01N27/44747 , G01N33/54353 , G01N33/553 , G01N2030/528
Abstract: A method that comprises providing a polymerized sol-gel material (PSG) and linking an enzyme to a surface of the PSG via covalent linkage is provided. The surface of the PSG is derivatized with a linker that comprises a functional group for linking itself to the surface of the PSG and a functional group for linking itself with the enzyme. The linked-enzyme PSG, or microreactor, is an effective means of at least partially digesting a substrate, such as a biological substrate. The activity of the enzyme of the microreactor may be significantly enhanced, up to 200-fold for example, relative to the activity of the enzyme free of the microreactor. The microreactor is thus an effective vehicle for digesting a substrate such as a biomolecule, a protein, an oligonucleotide, a peptide, a steroid, and/or an organic acid, after which, any remaining substrate and one or more digestion product(s) may be separated and detected. Microreactors and integrated devices that incorporate microreactors, such as columns, pipet tips, wells, and well-plates, are also provided.
Abstract translation: 提供了一种包括提供聚合的溶胶 - 凝胶材料（PSG）并通过共价键将酶与PSG的表面连接的方法。 PSG的表面用包含用于将其自身连接到PSG表面的官能团的接头衍生化，以及将其自身与酶连接的官能团。 连接酶PSG或微反应器是至少部分消化底物如生物底物的有效手段。 相对于不含微反应器的酶的活性，微反应器的酶的活性可以显着增强，例如高达200倍。 因此，微反应器是用于消化诸如生物分子，蛋白质，寡核苷酸，肽，类固醇和/或有机酸的底物的有效载体，之后，任何残留的底物和一种或多种消化产物， 可能被分离和检测。 还提供了包含微反应器的微反应器和集成器件，例如色谱柱，移液管吸头，孔和孔板。
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公开(公告)号：US20060076268A1
公开(公告)日：2006-04-13
申请号：US11231709
申请日：2005-09-20
Applicant: Richard Zare
Inventor： Richard Zare
CPC classification number: C10G15/08
Abstract: A more energy-efficient method of processing crude oil is achieved by viscoelastic shearing in order to increase the vapor pressure of the crude oil. This change in vapor pressure allows a more efficient separation of volatile components from non-volatile components in the crude oil. By optimizing the energy expenditure for shearing and the energy expenditure for separating the volatile components from the non-volatile components of the crude oil, while simultaneously removing the volatile components by distillation, one can reduce the overall energy input for the separation. Alternatively, it is possible to affect the distillation at a reduced temperature.
Abstract translation: 通过粘弹性剪切实现加工原油的更节能的方法，以增加原油的蒸汽压力。 蒸汽压力的这种变化允许挥发性组分与原油中非挥发性组分的更有效的分离。 通过优化用于剪切的能量消耗和用于将挥发性组分与原油的非挥发性组分分离的能量消耗，同时通过蒸馏除去挥发性组分，可以减少分离的总能量输入。 或者，可以在降低的温度下影响蒸馏。
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Patent Agency Ranking
公开(公告)号：US20060231751A1
公开(公告)日：2006-10-19
申请号：US11107583
申请日：2005-04-15
Applicant: Ignacio Zuleta , Richard Zare
Inventor： Ignacio Zuleta , Richard Zare
IPC: H01J49/00
CPC classification number: H01J49/061 , H01J49/0018 , H01L2924/0002 , H01L2924/00
Abstract: A beam modulation device gate is constructed from a silicon material, such as a silicon layer on an silicon on insulator wafer. The device further comprises a set of electrical contacts on the layer. The layer defines a set of electrically conducting silicon material fingers forming an array, wherein each of at least some of the fingers is connected electrically to one of the electrical contacts. The gate may be used in a mass or ion mobility spectrometer. Where the gate is constructed from a silicon on insulator wafer, an insulator layer supports the silicon layer and a handle layer supports the insulator layer. When predetermined electrical potentials are applied to the electrical contacts, at least some of the fingers will be substantially at said predetermined electrical potentials to modulate a beam of charged particles that passes through said array of fingers. A plurality of devices of the type above may be used, where each of the devices modulates the beam so that the beam is deflected along a direction different from direction along which the beam is deflected by any of the remaining devices. A plurality of devices of the type above may be used for a mass gate or charged particle buncher device. For making an ion optical device, an array of fingers is formed in a silicon layer of the silicon on insulator wafer. A portion of a handle layer of the wafer on a side of an insulator layer of the wafer opposite to that of the fingers is removed; and a portion of the insulator layer is removed so that the fingers are connected to the wafer only through the silicon layer and at one end of the fingers.
Abstract translation: 光束调制器件栅极由诸如硅绝缘体晶片上的硅层的硅材料构成。 该装置还包括该层上的一组电触点。 该层限定了一组形成阵列的导电硅材料指状物，其中至少一些指状物中的每一个与电触点之一电连接。 栅极可用于质谱或离子迁移谱仪。 在栅极由硅绝缘体晶片构成的情况下，绝缘体层支撑硅层，并且手柄层支撑绝缘体层。 当预定的电势被施加到电触点时，至少一些指状物将基本处于所述预定的电势，以调制穿过所述指状物阵列的带电粒子束。 可以使用上述类型的多个装置，其中每个装置调制光束，使得光束沿着不同于任何剩余装置偏转光束的方向的方向偏转。 上述类型的多个装置可以用于质量门或带电粒子聚束器装置。 为了制造离子光学器件，在硅绝缘体晶片的硅层中形成指状物阵列。 除去晶片的与手指相反的晶片的绝缘体层一侧的手柄层的一部分; 并且去除绝缘体层的一部分，使得指状物仅通过硅层和手指的一端连接到晶片。
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公开(公告)号：US20060192105A1
公开(公告)日：2006-08-31
申请号：US11411393
申请日：2006-04-25
Applicant: Richard Zare , Facundo Fernandez , Joel Kimmel , Oliver Trapp
Inventor： Richard Zare , Facundo Fernandez , Joel Kimmel , Oliver Trapp
CPC classification number: H01J49/061 , H01J49/40
Abstract: By connecting the Bradbury-Nielson gate (BNG) directly to a driver without a transmission line, distortion of the voltage waveform experienced a the BNG are much reduced. Because the magnitude of the modulation defects grows as the applied modulation voltage is increased, Bradbury-Nielson gates with finer wire spacing such as 100 microns, and operating at 10 to 15 V, significantly better signal-to-noise ratios are achieved. HT-TOFMS data were also post processed using an exact knowledge of the modulation defects.
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公开(公告)号：US20050269244A1
公开(公告)日：2005-12-08
申请号：US11129144
申请日：2005-05-13
Applicant: Richard Zare
Inventor： Richard Zare
Abstract: Improved crude oil processing is achieved by viscoelastic shearing of crude oil at an elevated temperature. The shearing provides for an enriched light fraction and an enriched heavy fraction that can be more efficiently separated by distillation. Shearing is achieved using a mobile surface and immobile surface, or other flow geometry, under conditions providing for a transient phase separation.
Abstract translation: 通过原油在高温下的粘弹性剪切实现改进的原油加工。 剪切提供富集的轻馏分和富集的重馏分，其可以通过蒸馏更有效地分离。 在提供瞬态相分离的条件下，使用移动表面和固定表面或其他流动几何形状实现剪切。
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8.发明申请Immobilized-enzyme microreactor devices for characterization of biomolecular analytes and associated methods 审中-公开用于表征生物分子分析物和相关方法的固定酶微反应器装置公开(公告)号：US20050127002A1
公开(公告)日：2005-06-16
申请号：US10734998
申请日：2003-12-12
Applicant: Richard Zare , Maria Dulay , Quentin Baca , Gabriele Trapp
Inventor： Richard Zare , Maria Dulay , Quentin Baca , Gabriele Trapp
CPC classification number: G01N33/573 , C12N11/08 , C12N11/14 , C12Q1/37 , G01N27/44747 , G01N33/54353 , G01N33/553 , G01N2030/528
Abstract: A method that comprises providing a polymerized sol-gel material (PSG) and linking an enzyme to a surface of the PSG via covalent linkage is provided. The surface of the PSG is derivatized with a linker that comprises a functional group for linking itself to the surface of the PSG and a functional group for linking itself with then enzyme. The linked-enzyme PSG, or microreactor, is an effective means of at least partially digesting a substrate, such as a biological substrate. The activity of the enzyme of the microreactor may be significantly enhanced, up to 200-fold for example, relative to the activity of the enzyme free of the microreactor. The microreactor is thus an effective vehicle for digesting a substrate such as a biomolecule, a protein, an oligonucleotide, a peptide, a steroid, and/or an organic acid, after which, any remaining substrate and one or more digestion product(s) may be separated and detected. Microreactors and integrated devices that incorporate microreactors, such as columns, pipet tips, wells, and well-plates, are also provided.
Abstract translation: 提供了一种包括提供聚合的溶胶 - 凝胶材料（PSG）并通过共价键将酶与PSG的表面连接的方法。 PSG的表面用包含用于将其自身连接到PSG表面的官能团的接头衍生化，以及将其自身与酶连接的官能团。 连接酶PSG或微反应器是至少部分消化底物如生物底物的有效手段。 相对于不含微反应器的酶的活性，微反应器的酶的活性可以显着增强，例如高达200倍。 因此，微反应器是用于消化诸如生物分子，蛋白质，寡核苷酸，肽，类固醇和/或有机酸的底物的有效载体，之后，任何残留的底物和一种或多种消化产物， 可能被分离和检测。 还提供了包含微反应器的微反应器和集成器件，例如色谱柱，移液管吸头，孔和孔板。
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公开(公告)号：US6020126A
公开(公告)日：2000-02-01
申请号：US821524
申请日：1997-03-21
Applicant: Christina Carlsson , Maria T. Dulay , Mats Jonsson , Peter Nielson , Bengt Norden , Lap-Chee Tsui , Richard Zare , Julian Zielinski
Inventor： Christina Carlsson , Maria T. Dulay , Mats Jonsson , Peter Nielson , Bengt Norden , Lap-Chee Tsui , Richard Zare , Julian Zielinski
CPC classification number: C07H21/00 , C12Q1/6813
Abstract: A rapid and sensitive detection method capable of reliably distinguishing fragments identical to a defined sequence from fragments containing a single base pair mismatch relative to the defined sequence. The method employs a peptide analog antisense probe, such as a peptide nucleic acid (PNA) probe, which hybridizes with DNA to forms complexes having higher thermal stability, but greater sensitivity to base pair mismatches, than corresponding DNA/DNA complexes. The method may be used in a number of applications, including genetic screening and hybridization sequencing applications.
Abstract translation: 一种快速且灵敏的检测方法，其能够将与所定义的序列相同的片段与相对于所定义的序列的单个碱基对失配的片段可靠地区分开。 该方法使用肽类似物反义探针，例如肽核酸（PNA）探针，其与DNA杂交以形成具有比相应的DNA / DNA复合物更高的热稳定性但对碱基对错配的较高敏感性的复合物。 该方法可用于许多应用，包括遗传筛选和杂交测序应用。
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