公开(公告)号：US20250152743A1

公开(公告)日：2025-05-15

申请号：US18945412

申请日：2024-11-12

Applicant: California Institute of Technology

Inventor： Michael B. Elowitz ,  Shiyu Xia ,  Andrew C. Lu ,  Lukas Moeller

IPC: A61K48/00 ,  C07K7/06 ,  C12N9/50

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in programmable cell death control. Compositions (e.g., nucleic acid compositions, synthetic protein circuits) provided herein can comprise one or more apoptosis polypeptides; and/or one or more pyroptosis polypeptides; and/or one or more input polypeptides. In some embodiments, expression of a synthetic protein circuit of the disclosure can induce, e.g., apoptosis and/or pyroptosis in a cell.

公开(公告)号：US20240124913A1

公开(公告)日：2024-04-18

申请号：US18486875

申请日：2023-10-13

Applicant: California Institute of Technology

Inventor： Andrew C. Lu ,  Michael J. Flynn ,  Lucy S. Chong ,  Ronghui Zhu ,  Michael B. Elowitz

IPC: C12Q1/37 ,  A61K38/48 ,  A61P35/00 ,  C12N9/22

CPC classification number: C12Q1/37 ,  A61K38/4873 ,  A61P35/00 ,  C12N9/22 ,  C12N2310/20

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in signal amplification. There are provided, in some embodiments, protease-based signal amplification modules. Disclosed herein include amplifier proteins comprising a first part of a first protease domain, a first dimerization domain, a first cut site a protease in a protease active state is capable of cutting, a second dimerization domain, a second cut site a protease in a protease active state is capable of cutting, and a first caging domain. Disclosed herein include companion amplifier proteins comprising a second part of a first protease domain, a third dimerization domain, a third cut site a protease in a protease active state is capable of cutting, a fourth dimerization domain, a fourth cut site a protease in a protease active state is capable of cutting, and a second caging domain.