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    SYNTHETIC CIRCUIT FOR CELLULAR MULTISTABILITY
    2.
    发明申请
    SYNTHETIC CIRCUIT FOR CELLULAR MULTISTABILITY 有权

    公开(公告)号：US20220177911A1

    公开(公告)日：2022-06-09

    申请号：US17544865

    申请日：2021-12-07

    Applicant: California Institute of Technology

    Inventor： Ronghui Zhu ,  Michael B. Elowitz

    IPC: C12N15/85 ,  C07K14/47

    Abstract: Disclosed herein include circuits, compositions, nucleic acids, populations, systems, and methods enabling single circuits to generate multiple molecularly and functionally distinct states that are each stable across multiple cell division cycles. Synthetic circuits provided herein can stably exist in multiple distinct states characterized by differences in the concentrations and expression levels of its components. In the absence of changes to the external environment, each of these states can be stable. In some embodiments, transcription factors provided herein activate when dimerized, and show much weaker activity as monomers. In some embodiments, each transcription factor homodimer activates expression of its own gene. In some embodiments, transcription factors can form mixed heterodimers with one another that do not strongly activate any genes in the circuit. Different embodiments of the synthetic circuits provided herein can use different numbers of transcription factors to produce a growing number of stable states.

    Protein-Based Signal Amplification
    5.
    发明公开
    Protein-Based Signal Amplification 审中-公开

    公开(公告)号：US20240124913A1

    公开(公告)日：2024-04-18

    申请号：US18486875

    申请日：2023-10-13

    Applicant: California Institute of Technology

    Inventor： Andrew C. Lu ,  Michael J. Flynn ,  Lucy S. Chong ,  Ronghui Zhu ,  Michael B. Elowitz

    IPC: C12Q1/37 ,  A61K38/48 ,  A61P35/00 ,  C12N9/22

    CPC classification number: C12Q1/37 ,  A61K38/4873 ,  A61P35/00 ,  C12N9/22 ,  C12N2310/20

    Abstract: Disclosed herein include methods, compositions, and kits suitable for use in signal amplification. There are provided, in some embodiments, protease-based signal amplification modules. Disclosed herein include amplifier proteins comprising a first part of a first protease domain, a first dimerization domain, a first cut site a protease in a protease active state is capable of cutting, a second dimerization domain, a second cut site a protease in a protease active state is capable of cutting, and a first caging domain. Disclosed herein include companion amplifier proteins comprising a second part of a first protease domain, a third dimerization domain, a third cut site a protease in a protease active state is capable of cutting, a fourth dimerization domain, a fourth cut site a protease in a protease active state is capable of cutting, and a second caging domain.

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