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公开(公告)号：US20140377742A1
公开(公告)日：2014-12-25
申请号：US14296199
申请日：2014-06-04
Applicant: California Institute of Technology
Inventor： Yu-Chong Tai , Siyang Zheng , Jeffrey Chun-Hui Lin , Harvey Kasdan
CPC classification number: B01L3/502761 , B01L3/502753 , B01L2200/0636 , B01L2200/0647 , B01L2300/0636 , B01L2300/0654 , B01L2300/0681 , B01L2300/0816 , B01L2300/0822 , B01L2300/0864 , B01L2300/0877 , B01L2400/086 , G01N15/1056 , G01N15/1484 , G01N33/5005 , G01N33/5094 , G01N33/582 , G01N2015/1006 , G01N2015/1486 , G01N2015/149 , Y10T436/11 , Y10T436/13 , Y10T436/25 , Y10T436/2575
Abstract: Described herein are particular embodiments relating to a microfluidic device that may be utilized for cell sensing, counting, and/or sorting. Particular aspects relate to a microfabricated device that is capable of differentiating single cell types from dense cell populations. One particular embodiment relates a device and methods of using the same for sensing, counting, and/or sorting leukocytes from whole, undiluted blood samples.
Abstract translation: 这里描述的是涉及可用于细胞感测，计数和/或分选的微流体装置的具体实施方案。 特定方面涉及能够将单细胞类型与致密细胞群分化的微加工装置。 一个具体实施方案涉及一种装置及其使用方法，用于从整个未稀释的血液样品中检测，计数和/或分选白细胞。
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公开(公告)号：US10371640B2
公开(公告)日：2019-08-06
申请号：US15647039
申请日：2017-07-11
Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventor： Yu-Chong Tai , Wendian Shi , Harvey Kasdan
Abstract: The present invention provide compositions and methods for classifying leukocytes in a leukocyte population using fluorescence detection. The methods include contacting a leukocyte population in a sample having one or more leukocyte types with a diagnostic composition, exciting the leukocyte population with a light source; and measuring emitted light from each of the one or more leukocyte types to classify the leukocyte population.
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公开(公告)号：US09029158B2
公开(公告)日：2015-05-12
申请号：US14296199
申请日：2014-06-04
Applicant: California Institute of Technology
Inventor： Yu-Chong Tai , Siyang Zheng , Jeffrey Chun-Hui Lin , Harvey Kasdan
CPC classification number: B01L3/502761 , B01L3/502753 , B01L2200/0636 , B01L2200/0647 , B01L2300/0636 , B01L2300/0654 , B01L2300/0681 , B01L2300/0816 , B01L2300/0822 , B01L2300/0864 , B01L2300/0877 , B01L2400/086 , G01N15/1056 , G01N15/1484 , G01N33/5005 , G01N33/5094 , G01N33/582 , G01N2015/1006 , G01N2015/1486 , G01N2015/149 , Y10T436/11 , Y10T436/13 , Y10T436/25 , Y10T436/2575
Abstract: Described herein are particular embodiments relating to a microfluidic device that may be utilized for cell sensing, counting, and/or sorting. Particular aspects relate to a microfabricated device that is capable of differentiating single cell types from dense cell populations. One particular embodiment relates a device and methods of using the same for sensing, counting, and/or sorting leukocytes from whole, undiluted blood samples.
Abstract translation: 这里描述的是涉及可用于细胞感测，计数和/或分选的微流体装置的具体实施方案。 特定方面涉及能够将单细胞类型与致密细胞群分化的微加工装置。 一个具体实施方案涉及一种装置及其使用方法，用于从整个未稀释的血液样品中检测，计数和/或分选白细胞。
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公开(公告)号：US20130137135A1
公开(公告)日：2013-05-30
申请号：US13686866
申请日：2012-11-27
Applicant: California Institute of Technology
Inventor： Yu-Chong Tai , Wendian Shi , Harvey Kasdan
IPC: G01N21/64
CPC classification number: G01N21/6486 , G01N21/05 , G01N21/6428 , G01N2021/0346 , G01N2021/6441 , G01N2021/6482
Abstract: The present invention provide compositions and methods for classifying leukocytes in a leukocyte population using fluorescence detection. The methods include contacting a leukocyte population in a sample having one or more leukocyte types with a diagnostic composition, exciting the leukocyte population with a light source; and measuring emitted light from each of the one or more leukocyte types to classify the leukocyte population.
Abstract translation: 本发明提供使用荧光检测对白细胞群体中的白细胞进行分类的组合物和方法。 所述方法包括将具有一种或多种白细胞类型的样品中的白细胞群与诊断组合物接触，用光源激发白细胞群; 并测量来自所述一种或多种白细胞类型中的每一种的发射光以分类白细胞群体。
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