公开(公告)号：US20170029444A1

公开(公告)日：2017-02-02

申请号：US15039674

申请日：2014-11-26

Applicant: DALIAN UNIVERSITY OF TECHNOLOGY

Inventor： Jiangli FAN ,  Hao ZHU ,  Xiaojun PENG ,  Jingyun WANG

IPC: C07F5/02 ,  G01N33/58 ,  C09K11/06 ,  G01N33/84

CPC classification number: C07F5/022 ,  C07F5/02 ,  C09K11/06 ,  C09K2211/1022 ,  C09K2211/1029 ,  G01N33/582 ,  G01N33/84

Abstract: The present invention provides a difluoroboron dipyrromethene fluorescent probe having a structure represented by the following general formula I, and a production method and an application thereof. Said difluoroboron dipyrromethene fluorescent probe exhibits low background fluorescence, and a rapid and significant fluorescence enhancement up to 100 times after the addition of hypochloric acid. The fluorescent intensity of said fluorescent probe shows a good linear relation with the concentration of hypochloric acid in a nanomole level, and the detection limit thereof is 0.56 nanomole. Said fluorescent probe has good selectivity since it hardly responds to other reactive oxygen species such as H2O2, O2−, TBHP, HO., TBO., 1O2 and NO., and is not interfered by pH in a wide range. Said fluorescent probe can be applied to detect hypochloric acid in living cells.

Abstract translation: 本发明提供具有由以下通式I表示的结构的二氟硼烷二吡咯亚甲基荧光探针及其制备方法和应用。 所述二氟硼酸二吡咯亚甲基荧光探针显示低背景荧光，并且在加入次氯酸之后快速且显着的荧光增强达100倍。 所述荧光探针的荧光强度与纳摩尔浓度的次氯酸浓度呈良好的线性关系，其检出限为0.56纳摩尔。 所述荧光探针具有良好的选择性，因为它几乎不响应其它活性氧物质如H 2 O 2，O 2 - ，TBHP，HO·，TBO·，1 O 2和NO-，并且不受宽泛的pH的干扰 范围。 所述荧光探针可用于检测活细胞中的次氯酸。