公开(公告)号：US09360455B2

公开(公告)日：2016-06-07

申请号：US14655263

申请日：2013-12-13

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： John L. Campbell ,  Yves Leblanc

IPC: G01N33/92 ,  G01N27/62 ,  H01J49/00 ,  H01J49/04

CPC classification number: G01N27/624 ,  G01N27/622 ,  G01N33/92 ,  G01N2458/15 ,  H01J49/0031 ,  H01J49/0422 ,  Y10T436/24

Abstract: A method for analyzing a sample that contains a plurality of lipid isomers is described that involves forming one or more lipid metal ion adducts and transporting the one or more lipid metal ion adducts through a differential mobility spectrometer to cause separation of the one or more lipid metal ion adducts from each other. The lipid isomers can be chosen, for example, from fatty acids, glycerolipids, glycerophospholipids, sphingolipids, saccharolipids, polyketides, sterol lipids, and prenol lipids. Particular examples include phosphatidylcholine regioisomers such as 1-palmatoyl-2-oleoyl-sn-phosphatidylcholine (POPC) and 1-oleoyl-2-palmatoyl-sn-phosphatidylcholine and triacylglycerols containing palmetic and oleic acid groups. The metal chosen can include a cationization reagent that contains sodium, potassium, silver or lithium.

Abstract translation: 描述了分析含有多种脂质异构体的样品的方法，其涉及形成一种或多种脂质金属离子加合物并将一种或多种脂质金属离子加合物输送通过差速迁移谱仪，以引起一种或多种脂质金属 离子加合物彼此。 脂质异构体可以选自例如脂肪酸，甘油脂，甘油磷脂，鞘脂，蔗糖脂，聚酮化合物，甾醇脂质和牛油醇脂质。 特别的实例包括磷脂酰胆碱区域异构体如1-棕榈酰-2-油酰-sn-磷脂酰胆碱（POPC）和1-油酰-2-棕榈酰-sn-磷脂酰胆碱和含有油酸和油酸基团的三酰基甘油。 所选择的金属可以包括含有钠，钾，银或锂的阳离子化试剂。