公开(公告)号：US20210120845A1

公开(公告)日：2021-04-29

申请号：US16074144

申请日：2017-02-21

Applicant: DUPONT NUTRITION BIOSCIENCES APS

Inventor： Karsten Matthias Kragh ,  Svend Haaning ,  Ernst Meinjohanns ,  Peter Edvard Degn ,  Steffan Yde Bak ,  Thomas Eisele

IPC: A23K20/189 ,  A23K10/14 ,  C12N9/48 ,  A23K20/147 ,  A23L2/66

Abstract: The present invention relates to compositions and methods for the production of a hydrolysate comprising at least one endoprotease and a tripeptidyl peptidase capable of cleaving tripeptides from the N-terminus a peptide and/or proteins having one or more of lysine, arginine or glycine in the P1 position wherein said tripeptidyl peptidase is capable of being used at a temperature between 45° C. and 70° C.

公开(公告)号：US20180295858A1

公开(公告)日：2018-10-18

申请号：US15520140

申请日：2015-10-23

Applicant: DUPONT NUTRITION BIOSCIENCES APS

Inventor： Svend Haaning ,  Karsten Matthias Kragh ,  Stepan Shipovskov ,  Andrei Miasnikov ,  Maria Ma ,  Luis Fernando Romero Millan ,  Luke Barnard ,  Shukun Yu ,  Ernst Meinjohanns ,  Steffen Bak ,  Päivi Nurminen ,  Heli Putaala

IPC: A23K10/14 ,  C12N9/48 ,  A23K20/147 ,  A23K50/75 ,  A23K50/30

Abstract: A method of preparing a feed additive composition comprising: (a) admixing at least one proline tolerant tripeptidyl peptidase predominantly having exopeptidase activity wherein said proline tolerant tripeptidyl peptidase is capable of cleaving tri-peptides from the N-terminus of peptides having (i) (A) Proline at P1; and (B) An amino acid selected from alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tryptophan, tyrosine, valine or synthetic amino acids at P1; or (ii) (a′) Proline at P1′; and (b′) An amino acid selected from alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tryptophan, tyrosine, valine or synthetic amino acids or amines at P1′; and one or more ingredients selected from the group consisting of a salt, polyol including sorbitol and glycerol, wheat or a wheat component, sodium acetate, sodium acetate trihydrate, potassium sorbate Talc, polyvinyl alcohol (PVA), benzoate, sorbiate, 1,3-propane diol, glucose, parabens, sodium chloride, citrate, metabisulfite, formate or a combination thereof; and (b) optionally packaging as well as uses of such proline tolerant tripeptidyl peptidases, feed additive compositions, feed additive kits, feeds or feedstuffs and/or premixes.

公开(公告)号：US20180291360A1

公开(公告)日：2018-10-11

申请号：US15574323

申请日：2016-06-27

Applicant: DUPONT NUTRITION BIOSCIENCES APS

Inventor： Karsten Matthias Kragh ,  Ernst Meinjohanns ,  Thomas Eisele ,  Peter Edvard Degn ,  Steffen Yde Bak

IPC: C12N9/48 ,  C12N15/80 ,  C12N1/14 ,  C12P21/06 ,  C12C5/00 ,  C12C1/047 ,  C12C11/00

Abstract: The present disclosure provides polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. In some embodiments, the disclosure also provides to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. In some embodiments, the present disclosure further provides to methods of obtaining protein hydrolysates useful as flavor improving agents.

公开(公告)号：US11473073B2

公开(公告)日：2022-10-18

申请号：US15574323

申请日：2016-06-27

Applicant: DUPONT NUTRITION BIOSCIENCES APS

Inventor： Karsten Matthias Kragh ,  Ernst Meinjohanns ,  Thomas Eisele ,  Peter Edvard Degn ,  Steffen Yde Bak

IPC: C12N9/48 ,  C12P21/06 ,  C12N15/80 ,  C12C1/047 ,  C12C5/00 ,  C12C11/00 ,  C12N1/14

Abstract: The present disclosure provides polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. In some embodiments, the disclosure also provides to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. In some embodiments, the present disclosure further provides to methods of obtaining protein hydrolysates useful as flavor improving agents.

公开(公告)号：US20200221715A1

公开(公告)日：2020-07-16

申请号：US16828172

申请日：2020-03-24

Applicant: DUPONT NUTRITION BIOSCIENCES APS

Inventor： Jens Frisbaek SØRENSEN ,  Rene Mikkelsen ,  Charlotte Horsmans Poulsen ,  Karsten Matthias Kragh

IPC: A21D2/18 ,  A21D8/04 ,  A21D13/02 ,  A21D13/062 ,  A23L7/10 ,  A23L7/104 ,  A23L7/109 ,  A23L7/122 ,  A23L7/13

Abstract: The present invention relates to the solubilisation of cereal bran, for preparing compositions comprising soluble fractions of cereal bran and the use of these compositions comprising solubilised cereal bran for the preparation of food products, such as bread.

公开(公告)号：US20240180172A1

公开(公告)日：2024-06-06

申请号：US18285530

申请日：2022-04-06

Applicant: DUPONT NUTRITION BIOSCIENCES APS

Inventor： Steven D. Doig ,  Ryan Lewis Frisch ,  Svend Haaning ,  Frank Wouter Koopman ,  Karsten Matthias Kragh ,  Lene Kragh ,  Chris Leeflang ,  Sina Pricelius

IPC: A21D8/04 ,  A21D2/18 ,  A23L29/00 ,  A23L29/30 ,  C12N9/26

CPC classification number: A21D8/042 ,  A21D2/186 ,  A23L29/06 ,  A23L29/30 ,  C12N9/2411 ,  C12Y302/01

Abstract: This invention relates to polypeptides, more specifically amylase polypeptides and nucleic acids encoding these, and their uses e.g. as non-maltogenic exoamy lases in producing food or feed products.

公开(公告)号：US20200236974A1

公开(公告)日：2020-07-30

申请号：US16637760

申请日：2018-07-18

Applicant: DUPONT NUTRITION BIOSCIENCES APS

Inventor： Shukun Yu ,  Karsten Matthias Kragh

IPC: A23K50/10 ,  A23K10/12 ,  C12N9/26 ,  C12N9/34

Abstract: Disclosed are methods for improving the digestibility of high-moisture grain feed and/or rehydrated grain feed for animals which comprises a) processing the grain feed into fragments and b) contacting the grain feed fragments of step (a) with at least one starch hydrolase which is stable and active at a pH less than 5.0, optionally at least one protease, in combination with at least one inoculant comprising at least one bacterial strain.

公开(公告)号：US20140363478A1

公开(公告)日：2014-12-11

申请号：US14222092

申请日：2014-03-21

Applicant: DUPONT NUTRITION BIOSCIENCES APS

Inventor： Charlotte Horsmans Poulsen ,  Karsten Matthias Kragh

IPC: A01N63/02

CPC classification number: A01N63/02 ,  C09D5/1606 ,  C12P3/00

Abstract: There is provided an anti-fouling composition comprising (i) a surface coating material; (ii) an enzyme obtained or obtainable from a marine organism; and (iii)(a) a substrate for the enzyme; and/or (b) a precursor enzyme and a precursor substrate, wherein the precursor enzyme and the precursor substrate are selected such that a substrate for the enzyme is generatable by action of the precursor enzyme on the precursor substrate; wherein the enzyme and the substrate are selected such that an anti-foulant compound is generatable by action of the enzyme on the substrate.

Abstract translation: 提供了防污组合物，其包含（i）表面涂层材料; （ii）从海洋生物获得或可获得的酶; 和（iii）（a）酶的底物; 和/或（b）前体酶和前体底物，其中前体酶和前体底物被选择为使得酶的底物可以通过前体酶在前体基质上的作用产生; 其中选择酶和底物使得抗污垢化合物可通过酶在底物上的作用产生。