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    NUCLEIC ACID DETECTION
    1.
    发明申请
    NUCLEIC ACID DETECTION 审中-公开
    核酸检测

    公开(公告)号：US20120183965A1

    公开(公告)日：2012-07-19

    申请号：US13261083

    申请日：2010-06-14

    Applicant: David Ward ,  David Edge ,  Nelson Nazareth

    Inventor： David Ward ,  David Edge ,  Nelson Nazareth

    IPC: G01N21/64 ,  C12M1/40 ,  G01N21/17

    CPC classification number: G01N21/6428 ,  B01L7/52 ,  B01L2300/0838 ,  B01L2400/0421 ,  C12Q1/686 ,  G01N21/645 ,  G01N27/447 ,  G01N27/44726 ,  G01N2021/0346 ,  G01N2021/6439 ,  G01N2021/6482 ,  C12Q2565/125 ,  C12Q2565/102 ,  C12Q2537/143

    Abstract: Apparatus for detecting a plurality of analytes in a sample and comprising: a reaction vessel; means for subjecting a sample in the reaction vessel to a multiplication process; a separation stage operable to separate amplimer constituents according to size; optical detection means for quantifying the sizes present and determining the colour of each size; and means for comparing the resulting quantification and colours with known data to determine the nature of the or each or a proportion of the or each target amplimers present. Processes employing the apparatus are also described.

    Abstract translation: 用于检测样品中多种分析物的装置，包括：反应容器; 用于使反应容器中的样品经受乘法的装置; 分离阶段，可操作以根据尺寸分离放大成分; 光学检测装置，用于量化存在的尺寸并确定每种尺寸的颜色; 以及用于将得到的定量和颜色与已知数据进行比较以确定存在的或每个或每个目标扩增子的比例的性质的装置。 还描述了使用该装置的过程。

    Wine bottle serving ware and manufacturing process
    2.
    发明申请
    Wine bottle serving ware and manufacturing process 审中-公开
    酒瓶服务和制造过程

    公开(公告)号：US20080035651A1

    公开(公告)日：2008-02-14

    申请号：US11890999

    申请日：2007-08-07

    Applicant: David Edge

    Inventor： David Edge

    IPC: A47G19/00

    CPC classification number: B65D1/0207 ,  B44C5/00

    Abstract: A glass wine bottle serving ware and the method of manufacturing that uses a standard glass wine bottle. The outside surface of the bottle is first treated with a cleaning-wetting solution that cleans and removes oil from the surface, and keeps the outside surface fog free. Mini egg shell frits and optional gold mica is then applied to the wet inside surface. A U-shaped copper wire is bent and attached to the top of the wine bottle that will serve as a device for hanging. The bottle is then placed inside a glass kiln. A piece of ceramic is placed under the neck of the bottle to create a bent handle when heating. The wine bottle is then heated in a sequential manner to create a flat, serving ware with a bent handle.

    Abstract translation: 玻璃酒瓶服务器和使用标准玻璃酒瓶的制造方法。 首先用清洁润湿溶液处理瓶的外表面，以清洁和去除表面上的油，并保持外表无雾。 然后将迷你蛋壳玻璃料和可选的金云母施加到湿的内表面。 U形铜线弯曲并附着在酒瓶的顶部，将作为悬挂装置。 然后将瓶子放置在玻璃窑内。 将一块陶瓷放置在瓶颈的下方，以在加热时产生弯曲的手柄。 然后将葡萄酒瓶按顺序加热，以形成具有弯曲手柄的平坦的服务器皿。

    Apparatus for and methods of the disruption of a biological cell
    8.
    发明授权
    Apparatus for and methods of the disruption of a biological cell 有权

    公开(公告)号：US11447767B2

    公开(公告)日：2022-09-20

    申请号：US13261540

    申请日：2011-06-15

    Applicant: Nelson Nazareth ,  David Ward ,  David Edge

    Inventor： Nelson Nazareth ,  David Ward ,  David Edge

    IPC: C12N15/10 ,  B01L7/00 ,  C12N1/06 ,  F25B21/04 ,  C12N1/08 ,  B01L3/00

    Abstract: Disrupting a biological cell includes freezing, boiling or perhaps alternately freezing and boiling material containing the biological cell using a thermoelectric cell with a working face, and a base face whereof is contiguous with a heat source/sink at a substantially constant temperature. Apparatus for the disruption process includes a peltier cell, a base face, which is flexibly attached to a heat source/sink held at a constant temperature, and a working face contiguous with a reaction vessel or holder thereof. Reversal of the voltage in the peltier cell enables the working face alternately to reach below freezing and above boiling temperatures, and/or with use of a resistive wire on the vessel or holder for heating, with the TEC used purely for cooling. The materials of the base face tend to inhibit disintegration of the peltier cell brought about by expansion and contraction by heat.

    CELL DISRUPTION
    10.
    发明申请
    CELL DISRUPTION 审中-公开
    细胞破坏

    公开(公告)号：US20130115670A1

    公开(公告)日：2013-05-09

    申请号：US13261540

    申请日：2011-06-15

    Applicant: Nelson Nazareth ,  David Ward ,  David Edge

    Inventor： Nelson Nazareth ,  David Ward ,  David Edge

    IPC: C12N15/10

    CPC classification number: C12N15/1003 ,  B01L3/5029 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2200/147 ,  B01L2300/044 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/049 ,  C12N1/066 ,  C12N1/08 ,  F25B21/04

    Abstract: A process for the disruption of a biological cell comprising freezing, boiling or perhaps alternately freezing and boiling the material containing the biological cell using a thermoelectric cell a base face whereof is contiguous with a heat sink/source held at a substantially constant temperature and a working face . Apparatus for carrying out the disruption process comprises a peltier cell a base face of which is flexibly attached to a heat sink arranged to be kept at a constant temperature of around 50° C. and a working face of which is contiguous with a reaction vessel or a reaction vessel holder. Reversal of the voltage in the peltier cell enables the working face alternately to reach below freezing and above boiling temperatures, and/or with use of a resistive wire on the vessel holder for heating with the TEC used purely for cooling The peltier cell base face is constructed of materials which tend to inhibit disintegration of the peltier cell brought about by expansion and contraction under heat.

    Abstract translation: 一种用于破坏生物细胞的方法，包括使用热电池冷冻，沸腾或者可能交替地冷冻和沸腾含有生物细胞的材料，其基面与保持在基本上恒定的温度的热沉/源连接，并且工作 脸。 用于进行破碎过程的装置包括一个珀耳帖细胞，其基面被柔性地附接到散热器，该散热器被设置成保持在约50℃的恒定温度，其工作面与反应容器相连， 反应容器支架。 在皮肤细胞中的电压反转使得工作面交替地达到低于冰点和沸点以上的温度，和/或使用电阻丝在容器保持器上用于纯化用于冷却的TEC加热珀耳帖细胞基面是 由倾向于抑制由加热引起的膨胀和收缩引起的珀耳帖细胞崩解的材料构成。

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