公开(公告)号：US09823127B2

公开(公告)日：2017-11-21

申请号：US14683066

申请日：2015-04-09

Applicant: Duke University

Inventor： Adam Wax ,  Howard Levinson ,  William J. Brown ,  Thomas Matthews ,  Manuel Medina

IPC: G01J3/28 ,  G01B9/02 ,  G01J3/453 ,  G01N21/45 ,  G01J3/02 ,  A61B5/00 ,  G01N21/47

CPC classification number: G01J3/2823 ,  A61B5/445 ,  G01B9/02044 ,  G01B9/02091 ,  G01J3/0218 ,  G01J3/453 ,  G01J3/4531 ,  G01N21/45 ,  G01N21/4795

Abstract: Disclosed herein are systems and methods for deep spectroscopic imaging of a biological sample. In an aspect, a system includes a broad bandwidth light source configured to generate an illumination beam, an interferometer, and a spectrometer. The interferometer includes a first beam splitter configured to split the illumination beam into an incident beam and a reference beam; an optical lens directs the incident beam onto a biological sample at a predefined offset from corresponding optical axis, and receive a beam scattered from the biological sample. The beams are configured to intersect with each other within a focal zone of the optical lens. Photons of the incident beam undergo multiple forward scattering within the biological sample. A second beam splitter configured to receive and superimpose the scattered and reference beams, to generate an interference beam. The spectrometer uses a spectral domain detection technique to assess tissue properties of the biological sample.

公开(公告)号：US20150285685A1

公开(公告)日：2015-10-08

申请号：US14683066

申请日：2015-04-09

Applicant: Duke University

Inventor： Adam Wax ,  Howard Levinson ,  William J. Brown ,  Thomas Matthews ,  Manuel Medina

IPC: G01J3/28 ,  G01J3/453 ,  G01N21/25 ,  G01B9/02

CPC classification number: G01J3/2823 ,  A61B5/445 ,  G01B9/02044 ,  G01B9/02091 ,  G01J3/0218 ,  G01J3/453 ,  G01J3/4531 ,  G01N21/45 ,  G01N21/4795

Abstract: Disclosed herein are systems and methods for deep spectroscopic imaging of a biological sample. In an aspect, a system includes a broad bandwidth light source configured to generate an illumination beam, an interferometer, and a spectrometer. The interferometer includes a first beam splitter configured to split the illumination beam into an incident beam and a reference beam; an optical lens directs the incident beam onto a biological sample at a predefined offset from corresponding optical axis, and receive a beam scattered from the biological sample. The beams are configured to intersect with each other within a focal zone of the optical lens. Photons of the incident beam undergo multiple forward scattering within the biological sample. A second beam splitter configured to receive and superimpose the scattered and reference beams, to generate an interference beam. The spectrometer uses a spectral domain detection technique to assess tissue properties of the biological sample.

Abstract translation: 本文公开了用于生物样品的深度光谱成像的系统和方法。 在一方面，系统包括配置成产生照明光束的宽带宽光源，干涉仪和光谱仪。 所述干涉仪包括：第一分束器，被配置为将照明光束分成入射光束和参考光束; 光学透镜将入射光束以预定偏移量从对应的光轴引导到生物样品上，并接收从生物样品散射的光束。 梁被构造成在光学透镜的聚焦区域内彼此相交。 入射光束的光子在生物样品内经历多次前向散射。 第二分束器，被配置为接收和叠加散射参考光束并产生干涉光束。 光谱仪使用光谱域检测技术来评估生物样品的组织性质。