公开(公告)号：US20170261431A1

公开(公告)日：2017-09-14

申请号：US15600245

申请日：2017-05-19

Applicant: GEN-PROBE INCORPORATED

Inventor： Gary D. LAIR ,  Thanh N. NGUYEN ,  Haitao LI ,  Florence LI ,  Byron J. KNIGHT ,  Robert E. HEINZ ,  Jerzy A. MACIOSZEK ,  Christopher B. DAVIS ,  Robert F. SCALESE

IPC: G01N21/64 ,  G01N35/02 ,  C12Q1/68 ,  G01N35/10 ,  G01N21/76 ,  G01N35/00 ,  G01N35/04

CPC classification number: G01N21/6428 ,  B01L7/52 ,  C12Q1/6813 ,  C12Q1/6851 ,  G01N21/6454 ,  G01N21/76 ,  G01N35/00693 ,  G01N35/0092 ,  G01N35/0098 ,  G01N35/0099 ,  G01N35/025 ,  G01N35/026 ,  G01N35/028 ,  G01N35/04 ,  G01N35/1002 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2035/00346 ,  G01N2035/00356 ,  G01N2035/00524 ,  G01N2035/0097 ,  G01N2035/0441 ,  G01N2035/0443 ,  G01N2035/0444 ,  G01N2035/0455 ,  G01N2035/0491 ,  G01N2035/103 ,  G01N2201/0415 ,  Y10S435/808 ,  Y10S435/809 ,  Y10T436/113332 ,  Y10T436/25 ,  C12Q2537/165

Abstract: A continuous process for performing multiple nucleic acid amplification assays, where at least a portion of a second subset of reaction mixtures are transferred to a heater while a first subset of reaction mixtures are being subjected to conditions for performing a nucleic acid amplification assay. During the process, a plurality of reaction mixtures from the first and second subsets of reaction mixtures are simultaneously subjected to conditions sufficient to perform multiple nucleic acid amplification assays in the reaction mixtures. The presence or absence of a target nucleic acid in the first subset of reaction mixtures is determined while the reaction mixtures are in the heater.

公开(公告)号：US20190049378A1

公开(公告)日：2019-02-14

申请号：US16014139

申请日：2018-06-21

Applicant: GEN-PROBE INCORPORATED

Inventor： Gary D. LAIR ,  Thanh N. NGUYEN ,  Haitao LI ,  Florence LI ,  Byron J. KNIGHT ,  Robert E. HEINZ ,  Jerzy A. MACIOSZEK ,  Christopher B. DAVIS ,  Robert F. SCALESE

IPC: G01N21/64 ,  G01N35/04 ,  G01N35/10 ,  C12Q1/6813 ,  C12Q1/6851 ,  G01N21/76 ,  G01N35/02 ,  G01N35/00 ,  B01L7/00

Abstract: A method of determining the presence or amount of a target nucleic acid in each of a plurality of reaction mixtures. In the method, a first plurality of reaction mixtures are provided to a heater and subjected to conditions for performing a first amplification reaction. The presence or amount of a first target nucleic acid in each of the first plurality of reaction mixtures is determined during the first amplification reaction. During the first amplification reaction, a second plurality of reaction mixtures are provided to the heater and subjected to conditions for performing a second amplification reaction. The presence or amount of a second target nucleic acid in each of the second plurality of reaction mixtures is determined during the second amplification reaction. At least a portion of the second plurality of reaction mixtures are removed from the heater during the second amplification reaction.