公开(公告)号：US20200264187A1

公开(公告)日：2020-08-20

申请号：US16067886

申请日：2016-12-26

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Mitsuharu MIWA

IPC: G01N33/58 ,  G01N21/64 ,  G06T7/00

Abstract: A living body observation device is a device that allows observation of a surface of a specimen, and includes an excitation light source that radiates excitation light including a wavelength between 300 rim and 650 nm, a filter that cuts the excitation light in observation light emitted from a specimen irradiated with the excitation light, a camera that captures the observation light and outputs image data, a data storage unit that stores an image of the observation light as a reference image, an image processing unit that acquires a target image that is an image of the observation light after acquisition of the reference image and generates an analysis image indicating change in light intensity of the target image with respect to the reference image, and a display that outputs the analysis image.

公开(公告)号：US20190113455A1

公开(公告)日：2019-04-18

申请号：US16089389

申请日：2017-03-23

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Mitsuharu MIWA ,  Takahiro SHIKAYAMA

IPC: G01N21/64

Abstract: A fluorescence observation method includes a fluorescence imaging step of imaging fluorescence from an observation target by an imaging portion. The fluorescence imaging step includes a darkening step of darkening a space between the observation target and an observation window of the imaging portion by pressing a tip end portion of a light shielding member to the observation target or a placement surface of the observation target, in a state where a base end portion of the light shielding member including a tubular main body portion is attached to the imaging portion to surround the observation window, and a focus adjustment step of adjusting a focus position of the imaging portion.

公开(公告)号：US20180080877A1

公开(公告)日：2018-03-22

申请号：US15558490

申请日：2015-03-17

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Kazumasa HIRAWAKE ,  Mitsuharu MIWA

IPC: G01N21/64 ,  H04N5/235

CPC classification number: G01N21/6456 ,  G01N21/6408 ,  G01N2201/0691 ,  G01N2201/0692 ,  H04N5/2351 ,  H04N5/2353

Abstract: A fluorescence image acquisition system is a system generating a fluorescence image by imaging fluorescence emitted from an observation object, the system including a light emitting device for iterating an output and stoppage of excitation light by emitting the excitation light toward the observation object during an ON period and stopping the emission of the excitation light during an OFF period, an imaging device having a light receiving unit including a plurality of pixels two-dimensionally arranged and an exposure control unit for controlling an exposure of the light receiving unit, and for outputting ON image data and OFF image data by capturing image of the observation object during each of the ON period and the OFF period, and a camera controller for generating fluorescence image data and setting an exposure time of the light receiving unit on the basis of the ON image data and the OFF image data.

公开(公告)号：US20180082411A1

公开(公告)日：2018-03-22

申请号：US15557873

申请日：2016-02-24

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Takayuki SATO ,  Mitsuharu MIWA ,  Kazumasa HIRAWAKE

IPC: G06T5/50 ,  A61B1/04

CPC classification number: G06T5/50 ,  A61B1/00009 ,  A61B1/043 ,  G01N21/6456 ,  G02B21/0076 ,  G06T2207/10064 ,  G06T2207/10152 ,  G06T2207/20221 ,  H04N5/2256

Abstract: A fluorescent image acquisition system includes a light emitting device which emits excitation light during ON period and stop the emission of the excitation light during OFF period, an imaging device which outputs ON image data and OFF image data, an input device which sets a coefficient, an image processing unit which creates fluorescent image data and background image data, and an identification unit which identifies fluorescent pixels and non-fluorescent pixels. In order to create a superimposed image, the image processing unit calculates values obtained by summing pixel values of fluorescent pixels of the fluorescent image data and pixel values of fluorescent pixels of the background image data at a ratio of 1:1, as pixel values of the fluorescent pixels and calculates values obtained by multiplying at least pixel values of the non-fluorescent pixels of the background image by the coefficient, as pixel values of the non-fluorescent pixels.

公开(公告)号：US20160041098A1

公开(公告)日：2016-02-11

申请号：US14780680

申请日：2013-12-18

Applicant: HAMAMATSU PHOTONICS K.K

Inventor： Kazumasa HIRAWAKE ,  Mitsuharu MIWA

IPC: G01N21/64

CPC classification number: G01N21/6456 ,  A61B1/00006 ,  A61B1/00009 ,  A61B1/043 ,  A61B1/0638 ,  A61B1/0646 ,  A61B5/0071 ,  G01N21/6486 ,  G01N2201/061 ,  G01N2201/062

Abstract: A fluorescence viewing device is configured to take a difference between one of an image of a first frame and an image of a second frame output from an imaging device and the other of the first frame image and the second frame image stored in an image storage means, whereby clear observed images can be obtained without influence of background light. In the fluorescence viewing device, an exposure time of a fluorescence image acquisition period and an exposure time of a background image acquisition period are different from each other. Since ON/OFF durations of excitation light also become asymmetric according to the asymmetry of the exposure times, the device can reduce user's feeling of strangeness due to blinking of the excitation light.

Abstract translation: 荧光观察装置被配置为取得第一帧的图像和从成像装置输出的第二帧的图像中的一个和存储在图像存储装置中的第一帧图像和第二帧图像中的另一个之间的差异 ，从而可以在不影响背景光的情况下获得清晰的观察图像。 在荧光观察装置中，荧光图像获取期间的曝光时间和背景图像获取期间的曝光时间彼此不同。 由于根据曝光时间的不对称性，激发光的ON / OFF持续时间也变得不对称，所以该装置可以减少用户由于激发光的闪烁而产生的陌生感。