公开(公告)号：US20160187327A1

公开(公告)日：2016-06-30

申请号：US15064815

申请日：2016-03-09

Applicant: Lab-on-a-bead AB

Inventor： Sven OSCARSSON ,  Kristofer ERIKSSON ,  Peter SVEDLINDH

IPC: G01N33/548 ,  C08B37/00 ,  B01J20/32 ,  B01J20/28 ,  B01J20/24 ,  G01N33/543 ,  B01J20/285

CPC classification number: C08B37/0039 ,  B01D15/3804 ,  B01D15/3885 ,  B01J20/24 ,  B01J20/28009 ,  B01J20/28016 ,  B01J20/285 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3217 ,  B01J20/3242 ,  B01J20/3265 ,  G01N33/5434 ,  G01N33/548

Abstract: A novel system for the analysis of molecules and cells, comprising clusters where a non-magnetic particle is supplemented with magnetic particles to form a characteristic pattern, fingerprint or bar code. Methods and devices for formation of such particles are also disclosed.

Abstract translation: 用于分析分子和细胞的新系统，包括其中非磁性颗粒补充有磁性颗粒以形成特征图案，指纹图案或条形码的簇。 还公开了形成这种颗粒的方法和装置。

公开(公告)号：US20220389053A1

公开(公告)日：2022-12-08

申请号：US17766366

申请日：2020-10-05

Applicant: LAB-ON-A-BEAD AB

Inventor： Veronique CHOTTEAU ,  Kristofer ERIKSSON ,  Sven OSCARSSON ,  Nils Arnold BRECHMANN

IPC: C07K1/22 ,  C12M1/00 ,  C12P21/02 ,  C12N13/00

Abstract: There is provided a process for the separation of molecules from a suspension comprising cells at a concentration of at least 56×106 cells/ml, comprising the steps of: a) providing magnetic particles having a specific interaction with said molecules to be separated, b) mixing the magnetic particles with the cell suspension containing the molecules, c) bringing the cell suspension in contact with a magnetic field provided by a magnetic separation device to collect the magnetic particles, d) decreasing or removing said magnetic field and collecting said magnetic particles carrying said molecules, and e) removing said molecules from said magnetic particles, to provide a concentrated fraction of said molecules, and/or provide partial or complete removal of impurities and cells from the fraction containing the molecules. Advantages include that the yield increases, the volume of the bioreactor and other equipment can be made smaller so that the process becomes more economical.

公开(公告)号：US20160185880A1

公开(公告)日：2016-06-30

申请号：US15064661

申请日：2016-03-09

Applicant: Lab-on-a-bead AB

Inventor： Sven OSCARSSON ,  Kristofer ERIKSSON ,  Peter SVEDLINDH

IPC: C08B37/00 ,  B01J20/32 ,  B01J20/285 ,  B01J20/28

CPC classification number: C08B37/0039 ,  B01D15/3804 ,  B01D15/3885 ,  B01J20/24 ,  B01J20/28009 ,  B01J20/28016 ,  B01J20/285 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3217 ,  B01J20/3242 ,  B01J20/3265 ,  G01N33/5434 ,  G01N33/548

Abstract: A method for the production of particles by reacting functional groups on the surface of non-magnetic porous particles with functional groups on the surface of magnetic particles to form a covalent bond, to obtain particles supplemented with magnetic particles covalently bound to the outer part of said particles. Advantages include an increased binding capacity.

Abstract translation: 通过使非磁性多孔颗粒的表面上的官能团与磁性颗粒表面上的官能团反应形成共价键来生成颗粒的方法，以获得补充有与所述的磁性颗粒的外部共价结合的磁性颗粒的颗粒 粒子。 优点包括增加绑定能力。

公开(公告)号：US20160184737A1

公开(公告)日：2016-06-30

申请号：US15064720

申请日：2016-03-09

Applicant: Lab-on-a-bead AB

Inventor： Sven OSCARSSON ,  Kristofer ERIKSSON ,  Per-Olov ERIKSSON

IPC: B01D15/38 ,  B01J20/282 ,  B01J20/28

CPC classification number: B01D15/3885 ,  B01J20/28009 ,  B01J20/282 ,  B01J20/289 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274

Abstract: A process for large scale separation of molecules comprising the steps of providing magnetic porous particles having an affinity to said molecules to be separated; mixing said magnetic porous particles with a solution containing said molecules; bringing said mixture in contact with a magnetic separation device comprising a flow channel and at least one magnetic element; removing said at least one magnetic element and collecting the magnetic porous particles carrying said molecules; separating said molecules from said magnetic porous particles; obtaining a concentrated fraction of said molecules; and recirculating the magnetic porous particles. A system for performing this process.

Abstract translation: 一种用于大规模分离分子的方法，包括以下步骤：提供对待分离的分子具有亲和力的磁性多孔颗粒; 将所述磁性多孔颗粒与含有所述分子的溶液混合; 使所述混合物与包括流动通道和至少一个磁性元件的磁分离装置接触; 去除所述至少一个磁性元件并收集携带所述分子的磁性多孔颗粒; 将所述分子与所述磁性多孔颗粒分离; 获得所述分子的浓缩级分; 并再循环磁性多孔颗粒。 用于执行此过程的系统。