公开(公告)号：US10822648B1

公开(公告)日：2020-11-03

申请号：US15664769

申请日：2017-07-31

Applicant: Labrador Diagnostics LLC

Inventor： Pranav Patel ,  Indira Wu ,  Aaron Richardson ,  Zahra Kamila Belhocine ,  Josephine Lee ,  Scott Tabakman

IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/6806

Abstract: Improved methods for amplifying target nucleic acid sequences are provided by 1) first amplifying the number of copies of target nucleic acid sequences in a sample by a first nucleic acid amplification method, and then 2) applying a second nucleic amplification method to the amplified sample, or aliquot thereof, further amplifying the number of copies of target sequences. In embodiments, a first nucleic acid amplification method is a thermocycling method, and a second nucleic acid amplification method is an isothermal method.

公开(公告)号：US20210147910A1

公开(公告)日：2021-05-20

申请号：US17083815

申请日：2020-10-29

Applicant: Labrador Diagnostics LLC

Inventor： Pranav Patel ,  Indira Wu ,  Aaron Richardson ,  Zahra Kamila Belhocine ,  Josephine Lee ,  Scott Tabakman

IPC: C12Q1/686 ,  C12Q1/6806

Abstract: Improved methods for amplifying target nucleic acid sequences are provided by 1) first amplifying the number of copies of target nucleic acid sequences in a sample by a first nucleic acid amplification method, and then 2) applying a second nucleic amplification method to the amplified sample, or aliquot thereof, further amplifying the number of copies of target sequences. In embodiments, a first nucleic acid amplification method is a thermocycling method, and a second nucleic acid amplification method is an isothermal method.