公开(公告)号：US20160281129A1

公开(公告)日：2016-09-29

申请号：US15030281

申请日：2014-10-16

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Sung Bae KIM ,  Hiroshi IZUMI ,  Hiroaki TAO ,  Masaki TORIMURA ,  Akihiro WAKISAKA

IPC: C12Q1/66 ,  C07D487/04 ,  C12N9/02

CPC classification number: C12Q1/66 ,  C07D241/38 ,  C07D487/04 ,  C12N9/0069 ,  C12Y113/12 ,  C12Y113/12007

Abstract: The invention relates to a bioluminescent substrate suitably usable in a series of artificial luciferases (ALuc), and the invention provides a wavelength-shifted spectrum with a selective high intensity luminescence and high luminescence stability obtained by the use of the substrate together with ALuc. The luminescent substrate for ALuc obtained by the invention can be included together with a suitable luminescence solution in a luminescence kit. The bioluminescent substrate for ALuc of the invention can exhibit unprecedented excellent luminescence specificity and functionality in the conventional bioluminescence probe, two-hybrid assay, bioluminescent capsule, and reporter gene assay.

Abstract translation: 本发明涉及适用于一系列人造荧光素酶（ALuc）的生物发光底物，本发明提供具有选择性高强度发光和通过使用该基质与ALuc得到的高发光稳定性的波长偏移光谱。 通过本发明获得的用于ALuc的发光底物可以与合适的发光溶液一起包含在发光试剂盒中。 用于本发明的ALuc的生物发光底物可以在常规生物发光探针，双杂交测定，生物发光胶囊和报告基因测定中显示出前所未有的优异的发光特异性和功能性。

公开(公告)号：US20130261029A1

公开(公告)日：2013-10-03

申请号：US13906818

申请日：2013-05-31

Applicant: National Institute of Advanced Industrial Science and Technology

Inventor： Hiroshi AOKI ,  Masaki TORIMURA ,  Hiroaki TAO ,  Takashi IKEDA

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00369 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00659 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/0268 ,  B01L9/54 ,  B01L9/547 ,  B01L2200/022 ,  B01L2300/0838 ,  G01N35/1067 ,  G01N35/1074 ,  Y10T436/2575

Abstract: A spotter that includes a plurality of spotting heads, each of the plurality of spotting heads having a discharging portion at a tip portion, the plurality of spotting heads form an m×n array (m, n>1) with m spotting heads arranged lengthwise and n spotting heads arranged crosswise; and a pitch varying mechanism configured to vary an array pitch of the plurality of spotting heads arrayed in a lengthwise direction and an array pitch of the plurality of spotting heads arrayed in a crosswise direction.

Abstract translation: 一种检测器，包括多个点样头，所述多个点样头中的每一个具有在尖端部分的排出部分，所述多个点样头形成m×n阵列（m，n> 1），其中m个点样头纵向排列 并且n个点头交叉排列; 以及变桨机构，被配置为改变沿横向方向排列的多个点样头的阵列间距和沿横向排列的多个点样头的阵列间距。