公开(公告)号：US20160281129A1

公开(公告)日：2016-09-29

申请号：US15030281

申请日：2014-10-16

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Sung Bae KIM ,  Hiroshi IZUMI ,  Hiroaki TAO ,  Masaki TORIMURA ,  Akihiro WAKISAKA

IPC: C12Q1/66 ,  C07D487/04 ,  C12N9/02

CPC classification number: C12Q1/66 ,  C07D241/38 ,  C07D487/04 ,  C12N9/0069 ,  C12Y113/12 ,  C12Y113/12007

Abstract: The invention relates to a bioluminescent substrate suitably usable in a series of artificial luciferases (ALuc), and the invention provides a wavelength-shifted spectrum with a selective high intensity luminescence and high luminescence stability obtained by the use of the substrate together with ALuc. The luminescent substrate for ALuc obtained by the invention can be included together with a suitable luminescence solution in a luminescence kit. The bioluminescent substrate for ALuc of the invention can exhibit unprecedented excellent luminescence specificity and functionality in the conventional bioluminescence probe, two-hybrid assay, bioluminescent capsule, and reporter gene assay.

Abstract translation: 本发明涉及适用于一系列人造荧光素酶（ALuc）的生物发光底物，本发明提供具有选择性高强度发光和通过使用该基质与ALuc得到的高发光稳定性的波长偏移光谱。 通过本发明获得的用于ALuc的发光底物可以与合适的发光溶液一起包含在发光试剂盒中。 用于本发明的ALuc的生物发光底物可以在常规生物发光探针，双杂交测定，生物发光胶囊和报告基因测定中显示出前所未有的优异的发光特异性和功能性。

公开(公告)号：US20180265850A1

公开(公告)日：2018-09-20

申请号：US15763779

申请日：2016-09-30

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Sung Bae KIM

IPC: C12N9/02 ,  G01N33/535 ,  G01N21/76

CPC classification number: C12N9/0069 ,  C07K19/00 ,  C07K2319/01 ,  C12N5/10 ,  C12N15/09 ,  C12Q1/26 ,  C12Y113/12005 ,  G01N21/763 ,  G01N33/535 ,  G01N33/543

Abstract: The present invention relates to provision of novel artificial bioluminescent enzymes. According to one embodiment of the present invention, there is provided a polypeptide, including any one of amino acid sequences (i) to (iii) below, and having a copepod luciferase activity: (i) an amino acid sequence represented by SEQ ID NO: 1 or 12; (ii) an amino acid sequence represented by SEQ ID NO: 1 or 12 in which one or several amino acids are substituted, added, or deleted; or (iii) an amino acid sequence having an identity of not less than 90% with an amino acid sequence represented by SEQ ID NO: 1 or 12.