公开(公告)号：US20230070781A1

公开(公告)日：2023-03-09

申请号：US17904383

申请日：2020-12-10

Applicant: National Institute of Advanced Industrial Science and Technology

Inventor： Yoshiyuki TERAMOTO ,  Masaki TORIMURA ,  Taizo SANO

IPC: G01J3/443 ,  G01N21/25 ,  G01N21/31

Abstract: A sample analysis system including: a droplet device that intermittently introduces a sample to a measurement region set in plasma; a light emission detection device that detects light emission in the measurement region at a detection timing, the detection timing being set at a predetermined cycle in advance; and an analysis device that analyzes the sample based on the detected light emission, the analysis device being provided with: a distribution computing unit that computes a time-spatial light intensity distribution based on the detected light emission, the time-spatial light intensity distribution being a distribution of a light intensity according to the detection timing, a position in the measurement region, and an wavelength component of the light emission; and a characteristic specifying unit that computes a feature amount that correlates with a sample characteristic indicating a property of the sample and specifies the sample characteristic based on the feature amount.

公开(公告)号：US20180217128A1

公开(公告)日：2018-08-02

申请号：US15746570

申请日：2016-07-06

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Hidenori TANI ,  Masaki TORIMURA ,  Hiroaki SATO

IPC: G01N33/50 ,  G01N33/542 ,  G01N33/53 ,  G01N33/58

Abstract: The present invention provides means for evaluating harmfulness of a chemical substance before occurrence of cell death, that is, more quickly and sensitively compared to conventional methods wherein the remaining viable cell count is determined using a reductive coloring reagent after a period of time required for occurrence of cell death due to the chemical substance. A double-stranded RNA probe comprising an RNA strand labeled with a fluorescent dye A that emits fluorescence, and an RNA strand labeled with a fluorescent dye B that quenches emission from a fluorescent dye in the vicinity thereof, wherein the fluorescence is quenched in a double-stranded state due to occurrence of fluorescence resonance energy transfer (FRET) between the two kinds of fluorescent dyes. When the probe is introduced into a cell and the cell is in a normal state, the double-stranded RNA is quickly degraded by activity of intracellular ribonuclease to cause cancellation of the FRET state, allowing fluorescence emission of the fluorescent dye A. On the other hand, in cases where the cell is harmfully influenced, activity of intracellular ribonuclease is suppressed, so that the double-stranded RNA remains undegraded, and the fluorescent dye A remains quenched due to the influence of the fluorescent dye B. By detecting a decrease in the degradation rate of intracellular RNA based on this principle, harmfulness of a chemical substance can be quickly and sensitively evaluated before occurrence of cell death.

公开(公告)号：US20160281129A1

公开(公告)日：2016-09-29

申请号：US15030281

申请日：2014-10-16

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Sung Bae KIM ,  Hiroshi IZUMI ,  Hiroaki TAO ,  Masaki TORIMURA ,  Akihiro WAKISAKA

IPC: C12Q1/66 ,  C07D487/04 ,  C12N9/02

CPC classification number: C12Q1/66 ,  C07D241/38 ,  C07D487/04 ,  C12N9/0069 ,  C12Y113/12 ,  C12Y113/12007

Abstract: The invention relates to a bioluminescent substrate suitably usable in a series of artificial luciferases (ALuc), and the invention provides a wavelength-shifted spectrum with a selective high intensity luminescence and high luminescence stability obtained by the use of the substrate together with ALuc. The luminescent substrate for ALuc obtained by the invention can be included together with a suitable luminescence solution in a luminescence kit. The bioluminescent substrate for ALuc of the invention can exhibit unprecedented excellent luminescence specificity and functionality in the conventional bioluminescence probe, two-hybrid assay, bioluminescent capsule, and reporter gene assay.

Abstract translation: 本发明涉及适用于一系列人造荧光素酶（ALuc）的生物发光底物，本发明提供具有选择性高强度发光和通过使用该基质与ALuc得到的高发光稳定性的波长偏移光谱。 通过本发明获得的用于ALuc的发光底物可以与合适的发光溶液一起包含在发光试剂盒中。 用于本发明的ALuc的生物发光底物可以在常规生物发光探针，双杂交测定，生物发光胶囊和报告基因测定中显示出前所未有的优异的发光特异性和功能性。

公开(公告)号：US20130261029A1

公开(公告)日：2013-10-03

申请号：US13906818

申请日：2013-05-31

Applicant: National Institute of Advanced Industrial Science and Technology

Inventor： Hiroshi AOKI ,  Masaki TORIMURA ,  Hiroaki TAO ,  Takashi IKEDA

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00369 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00659 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/0268 ,  B01L9/54 ,  B01L9/547 ,  B01L2200/022 ,  B01L2300/0838 ,  G01N35/1067 ,  G01N35/1074 ,  Y10T436/2575

Abstract: A spotter that includes a plurality of spotting heads, each of the plurality of spotting heads having a discharging portion at a tip portion, the plurality of spotting heads form an m×n array (m, n>1) with m spotting heads arranged lengthwise and n spotting heads arranged crosswise; and a pitch varying mechanism configured to vary an array pitch of the plurality of spotting heads arrayed in a lengthwise direction and an array pitch of the plurality of spotting heads arrayed in a crosswise direction.

Abstract translation: 一种检测器，包括多个点样头，所述多个点样头中的每一个具有在尖端部分的排出部分，所述多个点样头形成m×n阵列（m，n> 1），其中m个点样头纵向排列 并且n个点头交叉排列; 以及变桨机构，被配置为改变沿横向方向排列的多个点样头的阵列间距和沿横向排列的多个点样头的阵列间距。