公开(公告)号：US20240287346A1

公开(公告)日：2024-08-29

申请号：US18634755

申请日：2024-04-12

Applicant: NISSAN CHEMICAL CORPORATION

Inventor： Yoshiomi HIROI ,  Natsuki ABE ,  Taito NISHINO

IPC: C09D133/14 ,  B05D3/00 ,  B05D7/24 ,  C08G61/04 ,  C09D133/26 ,  C09D165/04 ,  C12M1/00 ,  C12M1/22 ,  C12M1/32 ,  C12M1/34 ,  C12M3/00 ,  G01N21/77

CPC classification number: C09D133/14 ,  B05D3/00 ,  B05D7/24 ,  C09D133/26 ,  C09D165/04 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12M23/10 ,  C12M23/12 ,  C12M23/20 ,  G01N21/7703 ,  C08G61/04 ,  C08G2261/12 ,  C08G2261/143 ,  C08G2261/147

Abstract: The invention provides a structural base that includes a coating film on at least a portion of the surface thereof, the coating film having a difference between the maximum film thickness and the minimum film thickness of not more than 1000 Å. A method of the invention produces such structural bases. The coating film includes a copolymer including a repeating unit containing a group represented by formula (a), and a repeating unit containing a group represented by formula (b):




wherein Ua1, Ua2, Ub1, Ub2, Ub3 and An− are as defined herein.

公开(公告)号：US20190233792A1

公开(公告)日：2019-08-01

申请号：US16305002

申请日：2017-05-23

Applicant: NISSAN CHEMICAL CORPORATION

Inventor： Yoshiomi HIROI ,  Natsuki ABE ,  Taito NISHINO

IPC: C12M1/00 ,  C09D133/14 ,  C09D143/02 ,  C09D201/02 ,  C09D201/04

CPC classification number: C12M23/20 ,  C08F220/34 ,  C08F230/02 ,  C09D133/14 ,  C09D143/02 ,  C09D201/025 ,  C09D201/04 ,  C12M3/00 ,  C08F2220/1825

Abstract: The present invention provides a cell culture vessel having a surface coated with a copolymer including a repeating unit containing a group represented by the following formula (a), a repeating unit containing a group represented by the following formula (b), and a repeating unit containing a group represented by the following formula (c) (wherein Ua1, Ua2, Ub1, Ub2, Ub3, Rc and An− are as defined in the description and the claims). The invention also provides a method for producing such cell culture vessels, and a method for producing a cell aggregate using the vessel.

公开(公告)号：US20220135806A1

公开(公告)日：2022-05-05

申请号：US17429975

申请日：2020-02-12

Applicant: NISSAN CHEMICAL CORPORATION

Inventor： Miya HIROI ,  Yoshiomi HIROI ,  Kohei SUZUKI ,  Takeaki SHOJI ,  Natsuki ABE ,  Hiroyuki NAKAJIMA

IPC: C09D4/00 ,  C08F220/28 ,  C08F220/36 ,  C08F220/34 ,  C09D133/14 ,  C12N5/073

Abstract: The invention provides a method for producing a copolymer-containing varnish using a monomer having a specific unsaturated bond which does not cause the problem of gelation, a method for producing a composition, a method for producing a coating film, preferably compatible with biomaterials. The method for producing a copolymer-containing varnish comprises copolymerizing a monomer mixture containing compounds of formulas (1) and (2): wherein R1 represents a hydrogen atom or a methyl group, R2 represents an alkylene group having 1 to 6 carbon atoms, n represents an integer of 1 to 30, R11 represents a hydrogen atom or a methyl group, and A1 represents a monovalent organic group having a cationic property, in a solvent for polymerization, in the presence of a radical polymerization initiator, wherein % by mass of the compound of formula (1) in a phosphorus-containing compound contained in the monomer mixture is 70% by mass or more.

公开(公告)号：US20210238329A1

公开(公告)日：2021-08-05

申请号：US17271154

申请日：2019-08-22

Applicant: NISSAN CHEMICAL CORPORATION

Inventor： Kohei SUZUKI ,  Yoshiomi HIROI ,  Natsuki ABE

IPC: C08F220/34 ,  C08J5/18 ,  C08F2/06 ,  C08F2/44 ,  C08F220/06 ,  C12N1/00 ,  C12M1/12

Abstract: The invention provides a method for producing a polymer used as a base film for cell culture which comprises (i) preparing a mixture containing (a) a monomer of formula (I): wherein Ua1 and Ua2 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, Ra1 represents a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, and Ra2 represents a linear or branched alkylene group having 1 to 5 carbon atoms, (b) a radical polymerization initiator, and (c) an organic solvent, and (ii) preparing a polymer by raising a temperature of the mixture under stirring to polymerize the monomer. The invention also provides methods of producing a base film for cell culture and a cell culture container.

公开(公告)号：US20240092955A1

公开(公告)日：2024-03-21

申请号：US18511083

申请日：2023-11-16

Applicant: NISSAN CHEMICAL CORPORATION

Inventor： Kohei SUZUKI ,  Yoshiomi HIROI ,  Natsuki ABE

IPC: C08F220/34 ,  C08F2/06 ,  C08F2/44 ,  C08F220/06 ,  C08J5/18 ,  C12M1/12 ,  C12N1/00

CPC classification number: C08F220/34 ,  C08F2/06 ,  C08F2/44 ,  C08F220/06 ,  C08J5/18 ,  C12M25/02 ,  C12N1/00

Abstract: The invention provides a method for producing a polymer used as a base film for cell culture which comprises (i) preparing a mixture containing (a) a monomer of formula (I):




wherein Ua1 and Ua2 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, Ra1 represents a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, and Ra2 represents a linear or branched alkylene group having 1 to 5 carbon atoms, (b) a radical polymerization initiator, and (c) an organic solvent, and (ii) preparing a polymer by raising a temperature of the mixture under stirring to polymerize the monomer. The invention also provides methods of producing a base film for cell culture and a cell culture container.

公开(公告)号：US20200291339A1

公开(公告)日：2020-09-17

申请号：US16650907

申请日：2018-09-26

Applicant: NISSAN CHEMICAL CORPORATION

Inventor： Yoshiomi HIROI ,  Kohei SUZUKI ,  Natsuki ABE

IPC: C12M1/00 ,  C09D185/02

Abstract: The invention relates to a cell culture container that inhibits adhesion of cells, a method for manufacturing the same and a method for producing cell aggregates using the same. The cell culture container has a coating film containing a copolymer having a recurring unit which contains a group represented by formula (a) and a recurring unit which contains a group represented by formula (b): wherein Ua1, Ua2, Ub1, Ub2 and Ub3 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, and An− represents an anion selected from the group consisting of a halide ion, an inorganic acid ion, a hydroxide ion and an isothiocyanate ion, on at least a part of a surface of the container, and the volume of the container is 20 μL or less.

公开(公告)号：US20190218413A1

公开(公告)日：2019-07-18

申请号：US16319293

申请日：2017-07-18

Applicant: NISSAN CHEMICAL CORPORATION

Inventor： Yoshiomi HIROI ,  Natsuki ABE ,  Taito NISHINO

IPC: C09D133/14 ,  C12M1/00 ,  C12M1/32 ,  C09D165/04 ,  G01N21/77 ,  C12M1/22 ,  C09D133/26 ,  C08G61/04

CPC classification number: C09D133/14 ,  B05D3/00 ,  B05D7/24 ,  C08G61/04 ,  C08G2261/12 ,  C08G2261/143 ,  C08G2261/147 ,  C09D133/26 ,  C09D165/04 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12M23/10 ,  C12M23/12 ,  C12M23/20 ,  G01N21/7703

Abstract: The invention provides a structural base that includes a coating film on at least a portion of the surface thereof, the coating film having a difference between the maximum film thickness and the minimum film thickness of not more than 1000 Å. A method of the invention produces such structural bases. The coating film includes a copolymer including a repeating unit containing a group represented by formula (a), and a repeating unit containing a group represented by formula (b): wherein Ua1, Ua2, Ub1, Ub2, Ub3 and An− are as defined herein.