公开(公告)号：US20240401093A1

公开(公告)日：2024-12-05

申请号：US18676494

申请日：2024-05-28

Applicant: Northwest University

Inventor： Weina Li ,  Daidi Fan

IPC: C12P15/00 ,  C12N9/42

Abstract: A β-glycosidase SS-BGL mutant for modifying ginsenoside and an application thereof are provided, which relate to the field of genetic engineering technologies. The β-glycosidase SS-BGL mutant is a mutant mutating asparagines at 128th position and 302th position of the amino acid sequence as shown in SEQ ID NO: 1 of β-glycosidase SS-BGL into aspartic acids respectively. The β-glucosidase SS-BGL mutant improves the thermal stability of the natural β-glucosidase SS-BGL at extremely high temperature, and is more conducive to the application of SS-BGL in ginsenoside preparation.

公开(公告)号：US12258598B2

公开(公告)日：2025-03-25

申请号：US18638690

申请日：2024-04-18

Applicant: Northwest university

Inventor： Weina Li ,  Daidi Fan

IPC: C12N9/26 ,  C12N9/42 ,  C12N15/70 ,  C12P15/00 ,  C12P33/20

Abstract: A β-glycosidase SS-BGL mutant for modifying ginsenoside and application thereof are provided, which relate to the field of genetic engineering technologies. The β-glycosidase SS-BGL mutant is β-glycosidase SS-BGL with the amino acid sequence as shown in SEQ ID NO: 1 having mutation at one or more sites. A single-site mutation includes: A1, making glutamine at 96th position of SEQ ID NO: 1 to be mutated to glutamic acid; A2, making asparagine at 97th position of SEQ ID NO: 1 to be mutated to aspartate; A3, making asparagine at 128th position of SEQ ID NO: 1 to be mutated to aspartate; A4, making asparagine at 302th position of SEQ ID NO: 1 to be mutated to aspartate. A double-site mutation is a combination of A3 and A4, a three-site mutation is a combination of A1, A2 and A4, and a four-site mutation is a combination of A1, A2, A3 and A4.

公开(公告)号：US12134789B2

公开(公告)日：2024-11-05

申请号：US18614748

申请日：2024-03-25

Applicant: Northwest university

Inventor： Weina Li ,  Daidi Fan ,  Chenhui Zhu

IPC: C12N9/10 ,  C12N15/70 ,  C12P17/06

Abstract: A glycosyltransferase BS-YjiC mutant, a construction method and an application thereof provided, relating to the field of genetic engineering technologies. The glycosyltransferase BS-YjiC mutant is obtained by performing site-directed mutation on amino acids at the 125th, 178th, 313th, 125th and 178th, or 125th and 313th positions of a wild-type glycosyltransferase BS-YjiC with the amino acid sequence as shown in SEQ ID NO: 1. Compared with the wild-type glycosyltransferase BS-YjiC, the glycosyltransferase BS-YjiC mutant is more suitable for catalyzing protopanaxadiol (PPD) to generate rare ginsenosides F12 and Rh2, which is more conducive to the flexibility of the production process.

公开(公告)号：US20240318153A1

公开(公告)日：2024-09-26

申请号：US18614748

申请日：2024-03-25

Applicant: Northwest university

Inventor： Weina Li ,  Daidi Fan ,  Chenhui Zhu

IPC: C12N9/10 ,  C12N15/70 ,  C12P17/06

CPC classification number: C12N9/1051 ,  C12N15/70 ,  C12P17/06 ,  C12Y204/01

Abstract: A glycosyltransferase BS-YjiC mutant, a construction method and an application thereof provided, relating to the field of genetic engineering technologies. The glycosyltransferase BS-YjiC mutant is obtained by performing site-directed mutation on amino acids at the 125th, 178th, 313th, 125th and 178th, or 125th and 313th positions of a wild-type glycosyltransferase BS-YjiC with the amino acid sequence as shown in SEQ ID NO: 1. Compared with the wild-type glycosyltransferase BS-YjiC, the glycosyltransferase BS-YjiC mutant is more suitable for catalyzing protopanaxadiol (PPD) to generate rare ginsenosides F12 and Rh2, which is more conducive to the flexibility of the production process.

公开(公告)号：US20240401016A1

公开(公告)日：2024-12-05

申请号：US18638690

申请日：2024-04-18

Applicant: Northwest university

Inventor： Weina Li ,  Daidi Fan

IPC: C12N9/42 ,  C12N15/70 ,  C12P33/20

Abstract: A β-glycosidase SS-BGL mutant for modifying ginsenoside and application thereof are provided, which relate to the field of genetic engineering technologies. The β-glycosidase SS-BGL mutant is β-glycosidase SS-BGL with the amino acid sequence as shown in SEQ ID NO: 1 having mutation at one or more sites. A single-site mutation includes: A1, making glutamine at 96th position of SEQ ID NO: 1 to be mutated to glutamic acid; A2, making asparagine at 97th position of SEQ ID NO: 1 to be mutated to aspartate; A3, making asparagine at 128th position of SEQ ID NO: 1 to be mutated to aspartate; A4, making asparagine at 302th position of SEQ ID NO: 1 to be mutated to aspartate. A double-site mutation is a combination of A3 and A4, a three-site mutation is a combination of A1, A2 and A4, and a four-site mutation is a combination of A1, A2, A3 and A4.

公开(公告)号：US12024601B2

公开(公告)日：2024-07-02

申请号：US17427296

申请日：2019-01-31

Applicant: NORTHWEST UNIVERSITY

Inventor： Daidi Fan ,  Yang Li ,  Chenhui Zhu ,  Chanyuan Yang ,  Liping Jia ,  Xiaoxuan Ma ,  Jianya Yan

IPC: C08J3/00 ,  A61L15/24 ,  A61L15/42 ,  C08J3/075 ,  C08J9/28 ,  C08L1/00 ,  C08L5/00 ,  C08L29/04

CPC classification number: C08J3/075 ,  A61L15/24 ,  A61L15/425 ,  C08J9/283 ,  C08L29/04 ,  C08J2201/0484 ,  C08J2207/10 ,  C08J2329/04 ,  C08J2401/28 ,  C08J2405/04 ,  C08J2405/08

Abstract: The present invention relates to a polyvinyl alcohol hydrogel having an asymmetric pore size. the pore size of the upper surface of the polyvinyl alcohol hydrogel is 1-30 μm, the pore size of lower surface thereof is 50-300 μm, and the pore size of the hydrogel gradually increases from the upper surface to the lower surface. The polyvinyl alcohol hydrogel in the present invention has excellent biocompatibility, and has functions of blocking bacteria, anti-adhesion, the absorption of exudate, promoting wound healing, observing in situ of wound healing process and the like.