公开(公告)号：US20240165263A1

公开(公告)日：2024-05-23

申请号：US18283699

申请日：2022-03-30

Applicant: NORTHWESTERN UNIVERSITY

Inventor： Chad A. Mirkin ,  Michelle Hope Teplensky ,  Michael Evangelopoulos ,  Shuya Wang

IPC: A61K47/69 ,  A61K39/00 ,  A61K39/385 ,  A61K47/54 ,  A61K47/55 ,  A61P37/04

CPC classification number: A61K47/6925 ,  A61K39/001114 ,  A61K39/385 ,  A61K47/549 ,  A61K47/55 ,  A61K47/554 ,  A61K47/6911 ,  A61K47/6937 ,  A61P37/04 ,  A61K2039/55561 ,  A61K2039/575 ,  A61K2039/70

Abstract: The disclosure is generally related to spherical nucleic acids (SNAs), nanostructures with a core surrounded by a radial presentation of oligonucleotides, that can target multiple classes of immune cells. Methods of making and using the nanoparticles are also provided herein. In some aspects, the disclosure provides a spherical nucleic acid (SNA) comprising: (a) a nanoparticle core; (b) a shell of oligonucleotides attached to the external surface of the nanoparticle core, the shell of oligonucleotides comprising one or more immunostimulatory oligonucleotides; and (c) a first antigen that is a major histocompatibility complex type I (MHC-I) antigen, and a second antigen that is a major histocompatibility complex type II (MHC-II) antigen.

公开(公告)号：US20210220454A1

公开(公告)日：2021-07-22

申请号：US16886712

申请日：2020-05-28

Applicant: NORTHWESTERN UNIVERSITY

Inventor： Chad A. Mirkin ,  Shuya Wang ,  Kacper Skakuj ,  Xiaoyi S. Hu

IPC: A61K39/00 ,  A61K39/39 ,  A61P35/00

Abstract: The disclosure is generally directed to immunostimulatory protein-core spherical nucleic acids (SNAs) comprising a protein core and a ratio of immunostimulatory and non-immunostimulatory strands, methods of making the immunostimulatory protein-core SNAs as well as their use.

公开(公告)号：US20230381306A1

公开(公告)日：2023-11-30

申请号：US18191517

申请日：2023-03-28

Applicant: NORTHWESTERN UNIVERSITY

Inventor： Chad A. Mirkin ,  Shuya Wang ,  Bin Zhang ,  Kacper Skakuj

IPC: A61K39/39 ,  A61P35/00 ,  A61K9/127 ,  A61K39/00 ,  A61K39/385 ,  G01N33/50

CPC classification number: A61K39/39 ,  A61P35/00 ,  A61K9/127 ,  A61K39/0011 ,  A61K39/385 ,  G01N33/5047 ,  A61K2039/55555 ,  A61K2039/55561 ,  A61K2039/627

Abstract: The present disclosure provides compositions and methods comprising spherical nucleic acid (SNA) components for use as immunotherapeutic agents. The disclosure provides a method comprising: treating a population of antigen presenting cells with a SNA comprising a nanoparticle, an antigen, and an adjuvant; and determining a time at which the population of antigen presenting cells presents a maximal signal that is indicative of antigen presentation by the antigen presenting cells and a time at which the population of antigen presenting cells presents a maximal co-stimulatory signal due to the adjuvant. The disclosure includes compositions that comprise a pharmaceutically acceptable carrier and a SNA of the disclosure, wherein the SNA comprises a nanoparticle, an oligonucleotide on the surface of the nanoparticle, and an antigen that is associated with the surface of the SNA via a linker. The disclosure additionally includes articles of manufacture and kits.

公开(公告)号：US20240398918A1

公开(公告)日：2024-12-05

申请号：US18784244

申请日：2024-07-25

Applicant: NORTHWESTERN UNIVERSITY

Inventor： Chad A. Mirkin ,  Shuya Wang ,  Kacper Skakuj ,  Xiaoyi S. Hu

IPC: A61K39/00 ,  A61K39/39 ,  A61P35/00

Abstract: The disclosure is generally directed to immunostimulatory protein-core spherical nucleic acids (SNAs) comprising a protein core and a ratio of immunostimulatory and non-immunostimulatory strands, methods of making the immunostimulatory protein-core SNAs as well as their use.

公开(公告)号：US20220370490A1

公开(公告)日：2022-11-24

申请号：US17749977

申请日：2022-05-20

Applicant: NORTHWESTERN UNIVERSITY

Inventor： Chad A. Mirkin ,  Ziyin Huang ,  Lisa Cole ,  Cassandra Elizabeth Callmann ,  Shuya Wang

IPC: A61K31/713 ,  A61P37/04 ,  A61K9/127

Abstract: The present disclosure is generally directed to spherical nucleic acids (SNAs) comprising multiple TLR agonists that enable the simultaneous activation of multiple TLR pathways for maximally synergistic immune activation. In some aspects, the present disclosure provides a spherical nucleic acid (SNA) comprising: (a) a nanoparticle core; (b) one or more toll-like receptor 3 (TLR3) agonists encapsulated in the nanoparticle core; and (c) a shell of oligonucleotides attached to the external surface of the nanoparticle core, the shell of oligonucleotides comprising one or more toll-like receptor 9 (TLR9) agonist oligonucleotides. Methods of using the SNAs are also provided herein.

公开(公告)号：US20200291394A1

公开(公告)日：2020-09-17

申请号：US16611548

申请日：2018-05-17

Applicant: Northwestern University

Inventor： Chad A. Mirkin ,  Kacper Skakuj ,  Shuya Wang

IPC: C12N15/11 ,  A61K39/00

Abstract: The present disclosure provides compositions and methods directed to combining spherical nucleic acid (SNA) components that are required for T-cell activation and proliferation.