公开(公告)号：US11186810B2

公开(公告)日：2021-11-30

申请号：US16084283

申请日：2016-03-16

Applicant: Shimadzu Corporation ,  Yokohama City University ,  Osaka University ,  CORNING INCORPORATED

Inventor： Tomoki Ohkubo ,  Masaki Kanai ,  Hirohisa Abe ,  Hideki Taniguchi ,  Masahiro Kinooka ,  Goro Kobayashi ,  Takanori Takebe

IPC: C12M1/00 ,  C12M3/00 ,  C12M3/06 ,  C12M1/34

Abstract: A cell culture container includes a storage container, a cell culture area, a culture solution supply unit, and a discharge unit. The cell culture area is a region for culturing cells, and is provided in the storage container. The culture solution supply unit supplies the culture solution into the cell culture area at a flow rate equal to or less than a predetermined flow rate. The discharge unit discharges the liquid in the cell culture area to the outside of the storage container. A rectification unit is a structure in which a plurality of outflow ports are uniformly arranged in a direction perpendicular to the flow direction of the culture solution from the culture solution supply unit to the discharge unit.

公开(公告)号：US20220113232A1

公开(公告)日：2022-04-14

申请号：US17268689

申请日：2019-08-13

Applicant: Osaka University

Inventor： Masahiro Kinooka

IPC: G01N1/38 ,  A01N1/02 ,  B01L3/02

Abstract: Provided is a distribution method with which particle components such as cells can be dispensed. In one aspect, provided is a method for distributing particles, the method including dispersing the particles in a plastic fluid, and dispensing the plastic fluid containing the particles. In another aspect, provided is a method for producing a product including a particle and a container for the particle, the method including dispensing a plastic fluid in which the particles are dispersed, from a storage part in which the plastic fluid is stored, to a plurality of the containers.

公开(公告)号：US10954487B2

公开(公告)日：2021-03-23

申请号：US16071969

申请日：2017-01-20

Applicant: Osaka University

Inventor： Masahiro Kinooka ,  Eiji Nagamori

IPC: C12N5/00 ,  C12M1/00 ,  C12N5/074 ,  C12M1/04 ,  C12M1/34 ,  C12N5/0735

Abstract: Provided is a cell culturing method in which physical stress is reduced and oxygen can be supplied. The invention relates to a cell culturing method including; arranging cells in a dispersed state in a culture medium which is a plastic fluid; and introducing air bubbles into said culture medium.

公开(公告)号：US20200017836A1

公开(公告)日：2020-01-16

申请号：US16471312

申请日：2017-12-19

Applicant: Osaka University

Inventor： Masahiro Kinooka ,  Meehae Kim ,  Yukako Fujinaga

IPC: C12N5/074

Abstract: Provided are a method for promoting the proliferation of pluripotent stem cells and a proliferation promoting composition used in the method. In one aspect, a composition for promoting the proliferation of pluripotent stem cells includes hemagglutinin or modified hemagglutinin. In another aspect, a method for promoting the proliferation of pluripotent stem cells includes culturing pluripotent stem cells in a culture medium to which the proliferation promoting composition of the present disclosure has been added.

公开(公告)号：US12173034B2

公开(公告)日：2024-12-24

申请号：US16766596

申请日：2018-11-22

Applicant: OSAKA UNIVERSITY

Inventor： Yukako Fujinaga ,  Sho Amatsu ,  Masahiro Kinooka

IPC: C07K14/33 ,  C12N15/85

Abstract: The present invention provides a miniaturized hemagglutinin complex protein having function inhibitory activity against E-cadherin, wherein (a) all or a part of at least HA1 subcomponent, and/or a part of HA3 subcomponent are/is deleted, (b) regions in HA2 and HA3 subcomponents that contribute to binding with E-cadherin are present, and (c) the HA3 subcomponent is derived from Clostridium botulinum type A or type B, and an E-cadherin function inhibitor containing the hemagglutinin complex protein.

公开(公告)号：US20230035766A1

公开(公告)日：2023-02-02

申请号：US17788269

申请日：2020-12-21

Applicant: Nihon Kohden Corporation ,  Osaka University

Inventor： Hodaka Makino ,  Hirotsugu Kubo ,  Masahiro Kinooka

IPC: C12M1/34 ,  C12M1/36

Abstract: The presently disclosed subject matter provides a cell culture evaluation system including a measurement unit, a first storage unit, a second storage unit, an evaluation unit and an output unit, wherein: the measurement unit includes a sensor that acquires evaluation target data about motion or a state of a culture tool; the first storage unit stores the evaluation target data; the second storage unit stores reference data; the evaluation unit compares the evaluation target data with the reference data so as to evaluate an execution state of a cell culture process; and the output unit outputs an evaluation result created by the evaluation unit.

公开(公告)号：US09822344B2

公开(公告)日：2017-11-21

申请号：US14655804

申请日：2013-12-26

Applicant: OSAKA UNIVERSITY

Inventor： Masahiro Kinooka ,  Meehae Kim ,  Yukako Fujinaga ,  Yo Sugawara

IPC: C12N5/074

CPC classification number: C12N5/0696 ,  C12N2501/58 ,  C12N2501/734 ,  C12N2501/998

Abstract: Provided is a method by which cells deviated from the undifferentiated state, which emerge in a colony during culture of stem cells having pluripotency, can be removed. In one aspect, provided is a method for culturing stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. In another aspect, provided is a method for removing cells deviated from the undifferentiated state, the cells being cells that have emerged or may possibly emerge during culture of stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. In still another aspect, provided is a method for maintaining the undifferentiated state of stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion.

公开(公告)号：US20170130206A1

公开(公告)日：2017-05-11

申请号：US15321835

申请日：2015-06-29

Applicant: Osaka University

Inventor： Masahiro Kinooka ,  Meehae Kim ,  Yukako Fujinaga ,  Yo Sugawara

IPC: C12N5/074 ,  C12N5/00 ,  C07K14/33

CPC classification number: C12N5/0696 ,  C07K14/33 ,  C07K19/00 ,  C12N1/00 ,  C12N5/0081 ,  C12N5/10 ,  C12N15/09 ,  C12N2501/998

Abstract: Provided are a novel hemagglutinin that can remove cells deviated from the undifferentiated state, the cells being cells that emerge in a colony during culture of stem cells having pluripotency, and a novel method for culturing stem cells having pluripotency, the method using hemagglutinin. Provided is a mutant hemagglutinin complex protein derived from Clostridium botulinum type B, the complex protein containing at least subcomponents HA2 and HA3 of hemagglutinin derived from Clostridium botulinum type B, and the complex protein containing amino acids constituting a glycosylation site, with at least one of the amino acids having been mutated. Provided is a method for culturing stem cells having pluripotency, the method including culturing the stem cell having pluriopotency in the presence of a mutant hemagglutinin complex protein of the present disclosure.

公开(公告)号：US11795438B2

公开(公告)日：2023-10-24

申请号：US16471312

申请日：2017-12-19

Applicant: Osaka University

Inventor： Masahiro Kinooka ,  Meehae Kim ,  Yukako Fujinaga

IPC: C12N5/074

CPC classification number: C12N5/0696 ,  C12N2501/998

Abstract: Provided are a method for promoting the proliferation of pluripotent stem cells and a proliferation promoting composition used in the method. In one aspect, a composition for promoting the proliferation of pluripotent stem cells includes hemagglutinin or modified hemagglutinin. In another aspect, a method for promoting the proliferation of pluripotent stem cells includes culturing pluripotent stem cells in a culture medium to which the proliferation promoting composition of the present disclosure has been added.

公开(公告)号：US11505774B2

公开(公告)日：2022-11-22

申请号：US16659825

申请日：2019-10-22

Applicant: NIHON KOHDEN CORPORATION ,  OSAKA UNIVERSITY

Inventor： Hodaka Makino ,  Hirotsugu Kubo ,  Tetsuya Ogawa ,  Masahiro Kinooka

IPC: C12M1/00 ,  C12M1/34 ,  C12M1/12

Abstract: A sample storage apparatus includes: a lid member; a sample storage container; first and second fluid sucking sections; first and second fluid discharging sections; first to fourth flow paths; and a first fluid identification sensor. The first flow path is connected to the first fluid sucking section, the second flow path is connected to the second fluid sucking section, the third flow path is connected to the first fluid discharging section, and the fourth flow path is connected to the second fluid discharging section. The first fluid identification sensor for identifying a fluid is disposed in the first flow path.