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    Mutant hemagglutinin complex protein, and method for culturing pluripotent stem cells using same
    4.
    发明授权
    Mutant hemagglutinin complex protein, and method for culturing pluripotent stem cells using same 有权

    公开(公告)号：US11230701B2

    公开(公告)日：2022-01-25

    申请号：US15321835

    申请日：2015-06-29

    Applicant: Osaka University

    Inventor： Masahiro Kinooka ,  Meehae Kim ,  Yukako Fujinaga ,  Yo Sugawara

    IPC: C12N5/074 ,  C07K14/33 ,  C07K19/00 ,  C12N15/09 ,  C12N5/10 ,  C12N1/00 ,  C12N5/00

    Abstract: Provided are a novel hemagglutinin that can remove cells deviated from the undifferentiated state, the cells being cells that emerge in a colony during culture of stem cells having pluripotency, and a novel method for culturing stem cells having pluripotency, the method using hemagglutinin. Provided is a mutant hemagglutinin complex protein derived from Clostridium botulinum type B, the complex protein containing at least subcomponents HA2 and HA3 of hemagglutinin derived from Clostridium botulinum type B, and the complex protein containing amino acids constituting a glycosylation site, with at least one of the amino acids having been mutated. Provided is a method for culturing stem cells having pluripotency, the method including culturing the stem cell having pluriopotency in the presence of a mutant hemagglutinin complex protein of the present disclosure.

    Method for Culturing Pluripotent Stem Cells
    6.
    发明申请
    Method for Culturing Pluripotent Stem Cells 有权
    培养多能干细胞的方法

    公开(公告)号：US20150329831A1

    公开(公告)日：2015-11-19

    申请号：US14655804

    申请日：2013-12-26

    Applicant: OSAKA UNIVERSITY

    Inventor： Masahiro Kinooka ,  Meehae Kim ,  Yukako Fujinaga ,  Yo Sugawara

    IPC: C12N5/074

    CPC classification number: C12N5/0696 ,  C12N2501/58 ,  C12N2501/734 ,  C12N2501/998

    Abstract: Provided is a method by which cells deviated from the undifferentiated state, which emerge in a colony during culture of stem cells having pluripotency, can be removed. In one aspect, provided is a method for culturing stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. In another aspect, provided is a method for removing cells deviated from the undifferentiated state, the cells being cells that have emerged or may possibly emerge during culture of stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. In still another aspect, provided is a method for maintaining the undifferentiated state of stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion.

    Abstract translation: 可以除去在具有多能性的干细胞的培养期间在殖民地中出现的未分化状态的细胞偏离的方法。 一方面，提供了培养具有多能性的干细胞的方法，所述方法包括在可抑制细胞粘附的物质存在下进行细胞培养。 在另一方面，提供了一种去除脱离未分化状态的细胞的方法，所述细胞是在具有多能性的干细胞的培养期间已经出现或可能出现的细胞，所述方法包括在可以存在的物质存在下进行细胞培养 抑制细胞粘附。 另一方面，提供一种用于维持具有多能性的干细胞的未分化状态的方法，所述方法包括在可抑制细胞 - 细胞粘附的物质存在下进行细胞培养。

    Hemagglutinin complex protein and application thereof
    7.
    发明授权
    Hemagglutinin complex protein and application thereof 有权

    公开(公告)号：US12173034B2

    公开(公告)日：2024-12-24

    申请号：US16766596

    申请日：2018-11-22

    Applicant: OSAKA UNIVERSITY

    Inventor： Yukako Fujinaga ,  Sho Amatsu ,  Masahiro Kinooka

    IPC: C07K14/33 ,  C12N15/85

    Abstract: The present invention provides a miniaturized hemagglutinin complex protein having function inhibitory activity against E-cadherin, wherein (a) all or a part of at least HA1 subcomponent, and/or a part of HA3 subcomponent are/is deleted, (b) regions in HA2 and HA3 subcomponents that contribute to binding with E-cadherin are present, and (c) the HA3 subcomponent is derived from Clostridium botulinum type A or type B, and an E-cadherin function inhibitor containing the hemagglutinin complex protein.

    Method for culturing pluripotent stem cells
    9.
    发明授权
    Method for culturing pluripotent stem cells 有权

    公开(公告)号：US09822344B2

    公开(公告)日：2017-11-21

    申请号：US14655804

    申请日：2013-12-26

    Applicant: OSAKA UNIVERSITY

    Inventor： Masahiro Kinooka ,  Meehae Kim ,  Yukako Fujinaga ,  Yo Sugawara

    IPC: C12N5/074

    CPC classification number: C12N5/0696 ,  C12N2501/58 ,  C12N2501/734 ,  C12N2501/998

    Abstract: Provided is a method by which cells deviated from the undifferentiated state, which emerge in a colony during culture of stem cells having pluripotency, can be removed. In one aspect, provided is a method for culturing stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. In another aspect, provided is a method for removing cells deviated from the undifferentiated state, the cells being cells that have emerged or may possibly emerge during culture of stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion. In still another aspect, provided is a method for maintaining the undifferentiated state of stem cells having pluripotency, the method including performing cell culture in the presence of a substance that can inhibit cell-cell adhesion.

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