公开(公告)号：US20240402488A1

公开(公告)日：2024-12-05

申请号：US18678100

申请日：2024-05-30

Applicant: Purdue Research Foundation

Inventor： Fang Huang ,  Peiyi Zhang

IPC: G02B27/00 ,  G01N21/64 ,  G02B21/16 ,  G02B21/36 ,  G02B26/08 ,  G06T5/20 ,  G06T5/60 ,  G06T5/80 ,  G06T7/00 ,  G06T7/11

Abstract: The invention generally relates to systems and method for deep learning driven adaptive optics for single molecule localization microscopy. In certain aspects, the invention provides a system comprising: an imaging apparatus configured for conducting single-molecule localization microscopy (SMLM), wherein the imaging apparatus comprises a deformable mirror and a dynamic filter; and a processor operably associated with the imaging apparatus. In certain embodiments, the processor is configured to: monitor individual emission patterns produced via the imaging apparatus from a plurality of different single molecules in a sample; infer shared wavefront distortion for each of the individual emission patterns; provide the shared wavefront distortion for each of the individual emission patterns through the dynamic filter; and operate the deformable mirror to compensate for sample induced aberrations.

公开(公告)号：US12002185B2

公开(公告)日：2024-06-04

申请号：US17309027

申请日：2019-06-10

Applicant: Purdue Research Foundation

Inventor： Peiyi Zhang ,  Fang Huang ,  Sheng Liu

IPC: G06T3/40 ,  G01J3/28 ,  G01J3/443 ,  G01N21/64 ,  G06N3/08 ,  G06T3/4046

CPC classification number: G06T3/4046 ,  G01J3/2823 ,  G01J3/443 ,  G01N21/6428 ,  G01N21/6458 ,  G06N3/08

Abstract: A fluorescent single molecule emitter simultaneously transmits its identity, location, and cellular context through its emission patterns. A deep neural network (DNN) performs multiplexed single-molecule analysis to enable retrieving such information with high accuracy. The DNN can extract three-dimensional molecule location, orientation, and wavefront distortion with precision approaching the theoretical limit of information content of the image which will allow multiplexed measurements through the emission patterns of a single molecule.

公开(公告)号：US20240062334A1

公开(公告)日：2024-02-22

申请号：US18493899

申请日：2023-10-25

Applicant: Purdue Research Foundation

Inventor： Peiyi Zhang ,  Fang Huang ,  Sheng Liu

IPC: G06T3/40 ,  G01J3/28 ,  G01J3/443 ,  G01N21/64 ,  G06N3/08

CPC classification number: G06T3/4046 ,  G01J3/2823 ,  G01J3/443 ,  G01N21/6428 ,  G01N21/6458 ,  G06N3/08

Abstract: A fluorescent single molecule emitter simultaneously transmits its identity, location, and cellular context through its emission patterns. A deep neural network (DNN) performs multiplexed single-molecule analysis to enable retrieving such information with high accuracy. The DNN can extract three-dimensional molecule location, orientation, and wavefront distortion with precision approaching the theoretical limit of information content of the image which will allow multiplexed measurements through the emission patterns of a single molecule.