公开(公告)号：US12259323B2

公开(公告)日：2025-03-25

申请号：US17862297

申请日：2022-07-11

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Gerard Schmid ,  Keith G. Fife ,  James Beach ,  Jason W. Sickler ,  Lawrence C. West ,  Paul E. Glenn ,  Kyle Preston ,  Farshid Ghasemi ,  Benjamin Cipriany ,  Jeremy Lackey

IPC: G01N21/64 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/77 ,  G01N33/543

Abstract: System and methods for analyzing single molecules and performing nucleic acid sequencing. An integrated device may include multiple pixels with sample wells configured to receive a sample, which when excited, emits radiation. The integrated device includes a surface having a trench region recessed from a portion of the surface and an array of sample wells, disposed in the trench region. The integrated device also includes a waveguide configured to couple excitation energy to at least one sample well in the array and positioned at a first distance from a surface of the trench region and at a second distance from the surface in a region separate from the trench region. The first distance is smaller than the second distance. The system also includes an instrument that interfaces with the integrated device. The instrument may include an excitation energy source for providing excitation energy to the integrated device by coupling to an excitation energy coupling region of the integrated device.

公开(公告)号：US20240420850A1

公开(公告)日：2024-12-19

申请号：US18640453

申请日：2024-04-19

Applicant: Quantum-Si Incorporated

Inventor： Marylens Hernandez ,  Umut Eser ,  Michael Meyer ,  Henri Lichenstein ,  Tian Xu ,  Jonathan M. Rothberg

IPC: G16H70/40 ,  G16H50/20 ,  G16H50/50

Abstract: Methods and apparatus for predicting an association between input data in a first modality and data in a second modality using a statistical model trained to represent interactions between data having a plurality of modalities including the first modality and the second modality, the statistical model comprising a plurality of encoders and decoders, each of which is trained to process data for one of the plurality of modalities, and a joint-modality representation coupling the plurality of encoders and decoders. The method comprises selecting, based on the first modality and the second modality, an encoder/decoder pair or a pair of encoders, from among the plurality of encoders and decoders, and processing the input data with the joint-modality representation and the selected encoder/decoder pair or pair of encoders to predict the association between the input data and the data in the second modality.

公开(公告)号：US20240068027A1

公开(公告)日：2024-02-29

申请号：US18188122

申请日：2023-03-22

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang ,  David Dodd

IPC: C12Q1/6876 ,  C07H19/073 ,  C07H19/173 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q1/6816

CPC classification number: C12Q1/6876 ,  C07H19/073 ,  C07H19/173 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6869

Abstract: Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleic acid connected to a nucleotide and two or more luminescent labels. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

公开(公告)号：US20230408411A1

公开(公告)日：2023-12-21

申请号：US18333952

申请日：2023-06-13

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Keith G. Fife ,  David M. Boisvert

IPC: G01N21/64 ,  H01L27/146 ,  H01L27/148 ,  G01S7/4865 ,  C12Q1/6869

CPC classification number: G01N21/6428 ,  G01N21/6408 ,  G01N21/6458 ,  H01L27/14603 ,  G01N21/645 ,  H01L27/14687 ,  H01L27/14812 ,  G01S7/4865 ,  C12Q1/6869 ,  G01S7/4863

Abstract: An integrated circuit includes a photodetection region configured to receive incident photons. The photodetection region is configured to produce a plurality of charge carriers in response to the incident photons. The integrated circuit also includes at least one charge carrier storage region. The integrated circuit also includes a charge carrier segregation structure configured to selectively direct charge carriers of the plurality of charge carriers into the at least one charge carrier storage region based upon times at which the charge carriers are produced.

公开(公告)号：US20230375475A1

公开(公告)日：2023-11-23

申请号：US18356150

申请日：2023-07-20

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Gerard Schmid ,  Keith G. Fife ,  James Beach ,  Jason W. Sickler ,  Lawrence C. West ,  Paul E. Glenn ,  Kyle Preston ,  Farshid Ghasemi ,  Benjamin Cipriany ,  Jeremy Lackey

IPC: G01N21/64 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/77 ,  G01N33/543

CPC classification number: G01N21/6428 ,  G01N21/648 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/6408 ,  G01N21/6454 ,  G01N21/6486 ,  G01N21/7743 ,  G01N33/54373 ,  G01N2021/6421 ,  G01N2021/6419

Abstract: System and methods for analyzing single molecules and performing nucleic acid sequencing. An integrated device may include multiple pixels with sample wells configured to receive a sample, which when excited, emits radiation. The integrated device includes a surface having a trench region recessed from a portion of the surface and an array of sample wells, disposed in the trench region. The integrated device also includes a waveguide configured to couple excitation energy to at least one sample well in the array and positioned at a first distance from a surface of the trench region and at a second distance from the surface in a region separate from the trench region. The first distance is smaller than the second distance. The system also includes an instrument that interfaces with the integrated device. The instrument may include an excitation energy source for providing excitation energy to the integrated device by coupling to an excitation energy coupling region of the integrated device.

公开(公告)号：US20230258862A1

公开(公告)日：2023-08-17

申请号：US18138700

申请日：2023-04-24

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabir ,  Gerard Schmid ,  Jason w. Sickler ,  Paul E. Glenn ,  Lawrence C. West ,  Kyle Preston ,  Alexander Gondarenko ,  Benjamin Cipriany ,  James Beach ,  Keith G. Fife ,  Farshid Ghasemi

IPC: G02B6/12 ,  G01N21/25 ,  G01N21/64 ,  G01N21/77 ,  G01M11/00 ,  G02B6/122 ,  G02B6/293 ,  G02B6/30

CPC classification number: G02B6/12016 ,  G01N21/253 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/7703 ,  G01M11/35 ,  G02B6/1228 ,  G02B6/29319 ,  G02B6/29344 ,  G02B6/305 ,  G02B2006/12147 ,  G02B2006/12195

Abstract: System and methods for optical power distribution to a large numbers of sample wells within an integrated device that can analyze single molecules and perform nucleic acid sequencing are described. The integrated device may include a grating coupler configured to receive an optical beam from an optical source and optical splitters configured to divide optical power of the grating coupler to waveguides of the integrated device positioned to couple with the sample wells. Outputs of the grating coupler may vary in one or more dimensions to account for an optical intensity profile of the optical source.

公开(公告)号：US11719635B2

公开(公告)日：2023-08-08

申请号：US17391473

申请日：2021-08-02

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Keith G. Fife ,  David M. Boisvert

IPC: G01N21/64 ,  H01L27/146 ,  C12Q1/6869 ,  H01L27/148 ,  G01S7/4865 ,  H04N25/77

CPC classification number: G01N21/6428 ,  C12Q1/6869 ,  G01N21/64 ,  G01N21/6408 ,  G01S7/4865 ,  H01L27/14603 ,  H01L27/14612 ,  H01L27/14643 ,  H01L27/14687 ,  H01L27/14689 ,  H01L27/14812 ,  H04N25/77 ,  G01N21/6458

Abstract: An integrated circuit includes a photodetection region configured to receive incident photons. The photodetection region is configured to produce a plurality of charge carriers in response to the incident photons. The integrated circuit includes at least one charge carrier storage region. The integrated circuit also includes a charge carrier segregation structure configured to selectively direct charge carriers of the plurality of charge carriers directly into the at least one charge carrier storage region based upon times at which the charge carriers are produced.

公开(公告)号：US11655504B2

公开(公告)日：2023-05-23

申请号：US16043547

申请日：2018-07-24

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang ,  David Dodd

IPC: C12Q1/6876 ,  C12Q1/6816 ,  C12Q1/6806 ,  C07H19/073 ,  C07H21/04 ,  C07H19/173 ,  C12Q1/6869

CPC classification number: C12Q1/6876 ,  C07H19/073 ,  C07H19/173 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2525/185 ,  C12Q2525/197 ,  C12Q2525/313 ,  C12Q2563/103 ,  C12Q2600/16 ,  C12Q1/6869 ,  C12Q2525/197 ,  C12Q2563/103 ,  C12Q2565/102 ,  C12Q1/6869 ,  C12Q2525/197 ,  C12Q2563/107 ,  C12Q2565/102

Abstract: Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleic acid connected to a nucleotide and two or more luminescent labels. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

公开(公告)号：US11613772B2

公开(公告)日：2023-03-28

申请号：US16751138

申请日：2020-01-23

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang ,  David Dodd

IPC: C12Q1/68 ,  G01N21/64 ,  C12Q1/6818 ,  C12Q1/6869

Abstract: Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleotide connected to a nucleic acid comprising a FRET label comprising at least three luminescent molecules. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

公开(公告)号：US20230058852A1

公开(公告)日：2023-02-23

申请号：US17690920

申请日：2022-03-09

Applicant: Quantum-Si Incorporated

Inventor： Brian Reed ,  Mohammad Wadud Bhuiya ,  Manjula Pandey ,  Jeremy Lackey ,  Jonathan M. Rothberg ,  Thomas Christian

IPC: C12N9/12 ,  C12Q1/6869 ,  C12P19/34 ,  C12N15/52

Abstract: Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.