公开(公告)号：US20210148904A1

公开(公告)日：2021-05-20

申请号：US16611563

申请日：2018-05-08

Applicant: RENSSELAER POLYTECHNIC INSTITUTE

Inventor： Seok-Joon Kwon ,  Jonathan Seth Dordick ,  Domyoung Kim ,  Jungbae Kim ,  Inseon Lee

IPC: G01N33/543 ,  G01N33/569 ,  C12Q1/26 ,  C12Q1/6851 ,  C12Q1/689

Abstract: Methods and systems are directed to multiplex detection of a bacterial pathogen in a sample. A first biotinylated lysin-derived cell wall binding domain is complexed with an avidin layer on a surface. A first bacterial pathogen detection complex including a second biotinylated lysin-derived cell wall binding domain, a detection domain, and an avidin linker complexed between the cell wall binding domain and the detection domain is also provided. The cell wall binding domains are derived from an endolysin, autolysin, bacteriocin, or exolysin, and are configured to bind a cell wall of a target bacterial pathogen. The detection domain includes one or more enzymes, fluorescent material, or DNA for emitting a signal for detection. Target bacterial pathogens present in a sample can thus be detected in a sandwich assay exhibiting increased selectivity and reduced limit of detection relative to traditional ELISA.

公开(公告)号：US20250114394A1

公开(公告)日：2025-04-10

申请号：US18597050

申请日：2024-03-06

Applicant: RENSSELAER POLYTECHNIC INSTITUTE

Inventor： Robert John LINHARDT ,  So-Young Kim ,  Weihua Jin ,  Jonathan Seth Dordick ,  Fuming Zhang ,  Seok-Joon Kwon ,  Paul S. Kwon ,  Keith Fraser

IPC: A61K31/727 ,  A61K31/737 ,  A61K45/06 ,  A61P31/04

Abstract: The composition inhibits severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) via competitive binding to SARS-COV-2 spike protein. The composition includes a plurality of sulfated glycosaminoglycans which bind to SARS-COV-2 spike protein, preventing binding to and uptake by host cells. The sulfated glycosaminoglycans, including N-, 2-O, 3-O, or 6-O sulfate groups, or combinations thereof, include heparins and fucoidans, such as those isolated from brown seaweed. The compositions show antiviral activity, with EC50 as low as 0.08 μM, and low cytotoxicity, making it promising for clinical use. While established SARS-COV-2 treatments such as remdesivir need to be administered intravenously, the compositions discussed herein are advantageously capable to being delivered as a nasal spray, metered dose inhaler, oral delivery, etc.

公开(公告)号：US20250090572A1

公开(公告)日：2025-03-20

申请号：US18899285

申请日：2024-09-27

Applicant: RENSSELAER POLYTECHNIC INSTITUTE

Inventor： Robert John LINHARDT ,  So-Young Kim ,  Weihua Jin ,  Jonathan Seth Dordick ,  Fuming Zhang ,  Seok-Joon Kwon ,  Paul S. Kwon ,  Keith Fraser

IPC: A61K31/737 ,  A61K31/727 ,  A61P31/14

Abstract: The composition inhibits severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) via competitive binding to SARS-CoV-2 spike protein. The composition includes a plurality of sulfated glycosaminoglycans which bind to SARS-CoV-2 spike protein, preventing binding to and uptake by host cells. The sulfated glycosaminoglycans, including N-, 2-O, 3-O, or 6-O sulfate groups, or combinations thereof, include heparins and fucoidans, such as those isolated from brown seaweed. The compositions show antiviral activity, with EC50 as low as 0.08 μM, and low cytotoxicity, making it promising for clinical use. While established SARS-CoV-2 treatments such as remdesivir need to be administered intravenously, the compositions discussed herein are advantageously capable to being delivered as a nasal spray, metered dose inhaler, oral delivery, etc.

公开(公告)号：US20190004057A1

公开(公告)日：2019-01-03

申请号：US15737352

申请日：2016-06-17

Applicant: NEUROLOGICAL SURGERY, P.C. ,  RENSSELAER POLYTECHNIC INSTITUTE

Inventor： Seok-Joon Kwon ,  Robert J. Linhardt ,  Jonathan S Dordick ,  William J. Sonstein ,  Fuming Zhang

IPC: G01N33/68 ,  G01N33/543

Abstract: The invention encompasses methods and test strips for detecting the presence of cerebrospinal fluid (CSF) in a biological sample comprising removing sialo-transferrin and selectively detecting or measuring asialo-transferrin in the biological sample.

公开(公告)号：US20180050056A1

公开(公告)日：2018-02-22

申请号：US15679709

申请日：2017-08-17

Applicant: KABIO R&D SOO Co., Ltd. ,  Rensselaer Polytechnic Institute

Inventor： Jong Hwan Kwak ,  Kyung Bok Lee ,  Jong-Hwan Park ,  Dong Hee Na ,  Seok-Joon Kwon ,  Robert J. Linhardt ,  Jonathan S. Dordick ,  Fuming Zhang

IPC: A61K31/7016 ,  A61K47/69

CPC classification number: A61K31/7016 ,  A61K47/595 ,  A61K47/6935

Abstract: Provided are a conjugate including a core and sialic acids or derivatives thereof bound to the surface of the core, and use thereof. The conjugate provided in the present invention binds with hemagglutinin on the surface of influenza virus to inhibit the course of infection of influenza virus, thereby preventing or treating infection of influenza virus and also preventing or treating infection of influenza virus resistant to antiviral agents. Accordingly, the conjugate may be widely used in the development of prophylactic or therapeutic agents for influenza virus infection.

公开(公告)号：US20220091118A1

公开(公告)日：2022-03-24

申请号：US17423939

申请日：2020-01-31

Applicant: NSPC Technologies, LLC ,  Rensselaer Polytechnic Institute ,  Gwangju Institute of Science and Technology

Inventor： Min-Gon Kim ,  Robert John Linhardt ,  William J. Sonstein ,  Jonathan S. Dordick ,  Seok-Joon Kwon ,  Jusung Oh

IPC: G01N33/558 ,  G01N33/68

Abstract: The invention encompasses methods and test strips for detecting the presence of cerebrospinal fluid (CSF) in a biological sample comprising removing sialo-transferrin and selectively detecting or measuring asialo-transferrin in the biological sample.

公开(公告)号：US12099057B2

公开(公告)日：2024-09-24

申请号：US16611563

申请日：2018-05-08

Applicant: RENSSELAER POLYTECHNIC INSTITUTE

Inventor： Seok-Joon Kwon ,  Jonathan Seth Dordick ,  Domyoung Kim ,  Jungbae Kim ,  Inseon Lee

IPC: G01N33/543 ,  C12Q1/26 ,  C12Q1/6851 ,  C12Q1/689 ,  G01N33/569

CPC classification number: G01N33/54306 ,  C12Q1/26 ,  C12Q1/6851 ,  C12Q1/689 ,  C12Y101/03004 ,  G01N33/56911

Abstract: Methods and systems are directed to multiplex detection of a bacterial pathogen in a sample. A first biotinylated lysin-derived cell wall binding domain is complexed with an avidin layer on a surface. A first bacterial pathogen detection complex including a second biotinylated lysin-derived cell wall binding domain, a detection domain, and an avidin linker complexed between the cell wall binding domain and the detection domain is also provided. The cell wall binding domains are derived from an endolysin, autolysin, bacteriocin, or exolysin, and are configured to bind a cell wall of a target bacterial pathogen. The detection domain includes one or more enzymes, fluorescent material, or DNA for emitting a signal for detection. Target bacterial pathogens present in a sample can thus be detected in a sandwich assay exhibiting increased selectivity and reduced limit of detection relative to traditional ELISA.

公开(公告)号：US11067581B2

公开(公告)日：2021-07-20

申请号：US15737352

申请日：2016-06-17

Applicant: NEUROLOGICAL SURGERY, P.C. ,  RENSSELAER POLYTECHNIC INSTITUTE

Inventor： Seok-Joon Kwon ,  Robert J. Linhardt ,  Jonathan S. Dordick ,  William J. Sonstein ,  Fuming Zhang

IPC: G01N33/543 ,  G01N33/68 ,  G01N33/558

Abstract: The invention encompasses methods and test strips for detecting the presence of cerebrospinal fluid (CSF) in a biological sample comprising removing sialo-transferrin and selectively detecting or measuring asialo-transferrin in the biological sample.

公开(公告)号：US10881673B2

公开(公告)日：2021-01-05

申请号：US15679709

申请日：2017-08-17

Applicant: Kabio R&D SOO Co., Ltd. ,  Rensselaer Polytechnic Institute

Inventor： Jong Hwan Kwak ,  Seok-Joon Kwon ,  Robert J. Linhardt ,  Jonathan S. Dordick ,  Fuming Zhang

IPC: A61K31/7016 ,  A61K47/59 ,  A61K47/69

Abstract: Provided are a conjugate including a core and sialic acids or derivatives thereof bound to the surface of the core, and use thereof. The conjugate provided in the present invention binds with hemagglutinin on the surface of influenza virus to inhibit the course of infection of influenza virus, thereby preventing or treating infection of influenza virus and also preventing or treating infection of influenza virus resistant to antiviral agents. Accordingly, the conjugate may be widely used in the development of prophylactic or therapeutic agents for influenza virus infection.