公开(公告)号：US20170049327A1

公开(公告)日：2017-02-23

申请号：US15243391

申请日：2016-08-22

Applicant: Robert R. Alfano

Inventor： ROBERT ALFANO ,  Cheng-hui Liu

IPC: A61B5/00 ,  A61B1/00 ,  A61B10/00 ,  A61B1/05 ,  A61B5/08 ,  A61B5/20 ,  A61B1/07 ,  A61B1/06

CPC classification number: A61B5/0075 ,  A61B1/00009 ,  A61B1/00082 ,  A61B1/00089 ,  A61B1/051 ,  A61B1/063 ,  A61B1/07 ,  A61B5/0042 ,  A61B5/0084 ,  A61B5/0086 ,  A61B5/0091 ,  A61B5/08 ,  A61B5/20 ,  A61B5/201 ,  A61B5/202 ,  A61B5/42 ,  A61B5/4306 ,  A61B5/4312 ,  A61B5/4318 ,  A61B5/7282 ,  A61B10/0041

Abstract: A method to detect vibrations associated with biomolecules in tissues and cellsuses Resonance Raman (RR) spectroscopy to measure specific biomolecules in tissue and cells signals. The changes of RR lines of key molecules present to the chemical conformations and change due to disease such as cancer and heart disease. Biomolecules are collagen, flavins, tryptophan, NADH, NAD, etc. The laser beams excite RR of vibration associated with absorption of the key native molecules in tissue (Tryptophan, NADH, Flavins, Collagen, carotenoids, porphyrins and others. The margin assessment and RR images in 2D and 3D regions are found by RR signals using position scanners. The intensity and the numbers of molecule fingerprints indicate the prescence of and the degree of the changes of chemical conformations.

Abstract translation: 检测与组织和细胞内的生物分子相关的振动的方法共振拉曼（RR）光谱法测量组织和细胞信号中的特定生物分子。 关键分子的RR线的变化由于诸如癌症和心脏病等疾病而呈现为化学构象和变化。 生物分子是胶原蛋白，黄素，色氨酸，NADH，NAD等。激光束激发与吸收组织中重要天然分子（色氨酸，NADH，Flavins，胶原，类胡萝卜素，卟啉等）相关的振动的RR，边缘评估和 使用位置扫描仪，通过RR信号发现2D和3D区域的RR图像，分子指纹的强度和数量表示化学构象的变化和变化程度。

公开(公告)号：US20170127947A1

公开(公告)日：2017-05-11

申请号：US15243207

申请日：2016-08-22

Applicant: ROBERT R. ALFANO

Inventor： ROBERT ALFANO ,  LINGYAN SHI ,  YURY BUDANSKY

IPC: A61B5/00 ,  G01N21/65

CPC classification number: A61B5/0075 ,  A61B5/0068 ,  G01N21/65 ,  G01N2021/655 ,  G01N2201/0612

Abstract: There is a need for a compact instrument and microscope that maps the vibration fingerprints of biomolecules and chemicals in a sample such as brain, breast, cervix, and arteries. One can use spontaneous Raman scattering to accomplish this; however, the problem is low scattering efficiency to 10−5. With the availability of continuous wave diode laser at numerous wavelengths from 375 nm-1800 nm for parametric nonlinear difference vibrational mixing to enhance Stimulated Raman process within materials. A seed beam at Raman frequency is used with pump laser beam. In this way one can map in 2D and 3D images of the vibrations associate with disease changes. Scanning a pair of laser beams can map the location of vibrations within cells, smears, membranes, arteries, and tissues of animal and human.